Supplementation of mitochondria from endometrial mesenchymal stem cells improves oocyte quality in aged mice

Maternal ageing is one of the major causes of reduced ovarian reserve and low oocyte quality in elderly women. Decreased oocyte quality is the main cause of age‐related infertility. Mitochondria are multifunctional energy stations that determine the oocyte quality. The mitochondria in aged oocytes d...

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Autores principales: Zhang, Qi, Hao, Jian‐Xiu, Liu, Bo‐Wen, Ouyang, Ying‐Chun, Guo, Jia‐Ni, Dong, Ming‐Zhe, Wang, Zhen‐Bo, Gao, Fei, Yao, Yuan‐Qing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9977672/
https://www.ncbi.nlm.nih.gov/pubmed/36480483
http://dx.doi.org/10.1111/cpr.13372
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author Zhang, Qi
Hao, Jian‐Xiu
Liu, Bo‐Wen
Ouyang, Ying‐Chun
Guo, Jia‐Ni
Dong, Ming‐Zhe
Wang, Zhen‐Bo
Gao, Fei
Yao, Yuan‐Qing
author_facet Zhang, Qi
Hao, Jian‐Xiu
Liu, Bo‐Wen
Ouyang, Ying‐Chun
Guo, Jia‐Ni
Dong, Ming‐Zhe
Wang, Zhen‐Bo
Gao, Fei
Yao, Yuan‐Qing
author_sort Zhang, Qi
collection PubMed
description Maternal ageing is one of the major causes of reduced ovarian reserve and low oocyte quality in elderly women. Decreased oocyte quality is the main cause of age‐related infertility. Mitochondria are multifunctional energy stations that determine the oocyte quality. The mitochondria in aged oocytes display functional impairments with mtDNA damage, which leads to reduced competence and developmental potential of oocytes. To improve oocyte quality, mitochondrial supplementation is carried out as a potential therapeutic approach. However, the selection of suitable cells as the source of mitochondria remains controversial. We cultivated endometrial mesenchymal stem cells (EnMSCs) from aged mice and extracted mitochondria from EnMSCs. To improve the quality of oocytes, GV oocytes were supplemented with mitochondria via microinjection. And MII oocytes from aged mice were fertilized by intracytoplasmic sperm injection (ICSI), combining EnMSCs' mitochondrial microinjection. In this study, we found that the mitochondria derived from EnMSCs could significantly improve the quality of aged oocytes. Supplementation with EnMSC mitochondria significantly increased the blastocyst ratio of MII oocytes from aged mice after ICSI. We also found that the birth rate of mitochondria‐injected ageing oocytes was significantly increased after embryo transplantation. Our study demonstrates that supplementation with EnMSC‐derived mitochondria can improve the quality of oocytes and promote embryo development in ageing mice, which might provide a prospective strategy for clinical treatment.
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spelling pubmed-99776722023-03-03 Supplementation of mitochondria from endometrial mesenchymal stem cells improves oocyte quality in aged mice Zhang, Qi Hao, Jian‐Xiu Liu, Bo‐Wen Ouyang, Ying‐Chun Guo, Jia‐Ni Dong, Ming‐Zhe Wang, Zhen‐Bo Gao, Fei Yao, Yuan‐Qing Cell Prolif Original Articles Maternal ageing is one of the major causes of reduced ovarian reserve and low oocyte quality in elderly women. Decreased oocyte quality is the main cause of age‐related infertility. Mitochondria are multifunctional energy stations that determine the oocyte quality. The mitochondria in aged oocytes display functional impairments with mtDNA damage, which leads to reduced competence and developmental potential of oocytes. To improve oocyte quality, mitochondrial supplementation is carried out as a potential therapeutic approach. However, the selection of suitable cells as the source of mitochondria remains controversial. We cultivated endometrial mesenchymal stem cells (EnMSCs) from aged mice and extracted mitochondria from EnMSCs. To improve the quality of oocytes, GV oocytes were supplemented with mitochondria via microinjection. And MII oocytes from aged mice were fertilized by intracytoplasmic sperm injection (ICSI), combining EnMSCs' mitochondrial microinjection. In this study, we found that the mitochondria derived from EnMSCs could significantly improve the quality of aged oocytes. Supplementation with EnMSC mitochondria significantly increased the blastocyst ratio of MII oocytes from aged mice after ICSI. We also found that the birth rate of mitochondria‐injected ageing oocytes was significantly increased after embryo transplantation. Our study demonstrates that supplementation with EnMSC‐derived mitochondria can improve the quality of oocytes and promote embryo development in ageing mice, which might provide a prospective strategy for clinical treatment. John Wiley and Sons Inc. 2022-12-08 /pmc/articles/PMC9977672/ /pubmed/36480483 http://dx.doi.org/10.1111/cpr.13372 Text en © 2022 The Authors. Cell Proliferation published by Beijing Institute for Stem Cell and Regenerative Medicine and John Wiley & Sons Ltd. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Zhang, Qi
Hao, Jian‐Xiu
Liu, Bo‐Wen
Ouyang, Ying‐Chun
Guo, Jia‐Ni
Dong, Ming‐Zhe
Wang, Zhen‐Bo
Gao, Fei
Yao, Yuan‐Qing
Supplementation of mitochondria from endometrial mesenchymal stem cells improves oocyte quality in aged mice
title Supplementation of mitochondria from endometrial mesenchymal stem cells improves oocyte quality in aged mice
title_full Supplementation of mitochondria from endometrial mesenchymal stem cells improves oocyte quality in aged mice
title_fullStr Supplementation of mitochondria from endometrial mesenchymal stem cells improves oocyte quality in aged mice
title_full_unstemmed Supplementation of mitochondria from endometrial mesenchymal stem cells improves oocyte quality in aged mice
title_short Supplementation of mitochondria from endometrial mesenchymal stem cells improves oocyte quality in aged mice
title_sort supplementation of mitochondria from endometrial mesenchymal stem cells improves oocyte quality in aged mice
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9977672/
https://www.ncbi.nlm.nih.gov/pubmed/36480483
http://dx.doi.org/10.1111/cpr.13372
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