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Current advancement in the application of prime editing
Prime editing (PE) is a precise genome manipulation technology based on the “search and replace” approach of the CRISPR-Cas9 system, while it does not require the exogenous donor DNA and the DNA double-strand breaks (DSBs). Comparing the base editing technology, the editing scope of prime editing ha...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2023
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9978821/ https://www.ncbi.nlm.nih.gov/pubmed/36873365 http://dx.doi.org/10.3389/fbioe.2023.1039315 |
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author | Huang, Zhangrao Liu, Gang |
author_facet | Huang, Zhangrao Liu, Gang |
author_sort | Huang, Zhangrao |
collection | PubMed |
description | Prime editing (PE) is a precise genome manipulation technology based on the “search and replace” approach of the CRISPR-Cas9 system, while it does not require the exogenous donor DNA and the DNA double-strand breaks (DSBs). Comparing the base editing technology, the editing scope of prime editing has been widely expanded. Prime editing has been successfully applied in a variety of plant cells, animal cells and the model microorganism Escherichia coli so far, and it has shown a good application potential in breeding and genomic functional study of animals and plants, disease treatment, and modification of the microbial strains. In this paper, the basic strategies of prime editing are briefly described, and its research progress is summarized and prospected from the application of multiple species. In addition, a variety of optimization strategies for improving its efficiency and specificity of prime editing are outlined. |
format | Online Article Text |
id | pubmed-9978821 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-99788212023-03-03 Current advancement in the application of prime editing Huang, Zhangrao Liu, Gang Front Bioeng Biotechnol Bioengineering and Biotechnology Prime editing (PE) is a precise genome manipulation technology based on the “search and replace” approach of the CRISPR-Cas9 system, while it does not require the exogenous donor DNA and the DNA double-strand breaks (DSBs). Comparing the base editing technology, the editing scope of prime editing has been widely expanded. Prime editing has been successfully applied in a variety of plant cells, animal cells and the model microorganism Escherichia coli so far, and it has shown a good application potential in breeding and genomic functional study of animals and plants, disease treatment, and modification of the microbial strains. In this paper, the basic strategies of prime editing are briefly described, and its research progress is summarized and prospected from the application of multiple species. In addition, a variety of optimization strategies for improving its efficiency and specificity of prime editing are outlined. Frontiers Media S.A. 2023-02-16 /pmc/articles/PMC9978821/ /pubmed/36873365 http://dx.doi.org/10.3389/fbioe.2023.1039315 Text en Copyright © 2023 Huang and Liu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Bioengineering and Biotechnology Huang, Zhangrao Liu, Gang Current advancement in the application of prime editing |
title | Current advancement in the application of prime editing |
title_full | Current advancement in the application of prime editing |
title_fullStr | Current advancement in the application of prime editing |
title_full_unstemmed | Current advancement in the application of prime editing |
title_short | Current advancement in the application of prime editing |
title_sort | current advancement in the application of prime editing |
topic | Bioengineering and Biotechnology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9978821/ https://www.ncbi.nlm.nih.gov/pubmed/36873365 http://dx.doi.org/10.3389/fbioe.2023.1039315 |
work_keys_str_mv | AT huangzhangrao currentadvancementintheapplicationofprimeediting AT liugang currentadvancementintheapplicationofprimeediting |