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Current advancement in the application of prime editing

Prime editing (PE) is a precise genome manipulation technology based on the “search and replace” approach of the CRISPR-Cas9 system, while it does not require the exogenous donor DNA and the DNA double-strand breaks (DSBs). Comparing the base editing technology, the editing scope of prime editing ha...

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Detalles Bibliográficos
Autores principales: Huang, Zhangrao, Liu, Gang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9978821/
https://www.ncbi.nlm.nih.gov/pubmed/36873365
http://dx.doi.org/10.3389/fbioe.2023.1039315
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author Huang, Zhangrao
Liu, Gang
author_facet Huang, Zhangrao
Liu, Gang
author_sort Huang, Zhangrao
collection PubMed
description Prime editing (PE) is a precise genome manipulation technology based on the “search and replace” approach of the CRISPR-Cas9 system, while it does not require the exogenous donor DNA and the DNA double-strand breaks (DSBs). Comparing the base editing technology, the editing scope of prime editing has been widely expanded. Prime editing has been successfully applied in a variety of plant cells, animal cells and the model microorganism Escherichia coli so far, and it has shown a good application potential in breeding and genomic functional study of animals and plants, disease treatment, and modification of the microbial strains. In this paper, the basic strategies of prime editing are briefly described, and its research progress is summarized and prospected from the application of multiple species. In addition, a variety of optimization strategies for improving its efficiency and specificity of prime editing are outlined.
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spelling pubmed-99788212023-03-03 Current advancement in the application of prime editing Huang, Zhangrao Liu, Gang Front Bioeng Biotechnol Bioengineering and Biotechnology Prime editing (PE) is a precise genome manipulation technology based on the “search and replace” approach of the CRISPR-Cas9 system, while it does not require the exogenous donor DNA and the DNA double-strand breaks (DSBs). Comparing the base editing technology, the editing scope of prime editing has been widely expanded. Prime editing has been successfully applied in a variety of plant cells, animal cells and the model microorganism Escherichia coli so far, and it has shown a good application potential in breeding and genomic functional study of animals and plants, disease treatment, and modification of the microbial strains. In this paper, the basic strategies of prime editing are briefly described, and its research progress is summarized and prospected from the application of multiple species. In addition, a variety of optimization strategies for improving its efficiency and specificity of prime editing are outlined. Frontiers Media S.A. 2023-02-16 /pmc/articles/PMC9978821/ /pubmed/36873365 http://dx.doi.org/10.3389/fbioe.2023.1039315 Text en Copyright © 2023 Huang and Liu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Bioengineering and Biotechnology
Huang, Zhangrao
Liu, Gang
Current advancement in the application of prime editing
title Current advancement in the application of prime editing
title_full Current advancement in the application of prime editing
title_fullStr Current advancement in the application of prime editing
title_full_unstemmed Current advancement in the application of prime editing
title_short Current advancement in the application of prime editing
title_sort current advancement in the application of prime editing
topic Bioengineering and Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9978821/
https://www.ncbi.nlm.nih.gov/pubmed/36873365
http://dx.doi.org/10.3389/fbioe.2023.1039315
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