Maturation strategy influences expression levels and cofactor occupancy in Fe–S proteins

Iron–sulfur clusters are ubiquitous cofactors required for fundamental biological processes. Structural and spectroscopic analysis of Fe–S proteins is often limited by low cluster occupancy in recombinantly produced proteins. In this work, we report a systematic comparison of different maturation st...

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Detalles Bibliográficos
Autores principales: Jansing, Melissa, Mielenbrink, Steffen, Rosenbach, Hannah, Metzger, Sabine, Span, Ingrid
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer International Publishing 2022
Materias:
Original Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9981529/
https://www.ncbi.nlm.nih.gov/pubmed/36527507
http://dx.doi.org/10.1007/s00775-022-01972-1

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9981529/
https://www.ncbi.nlm.nih.gov/pubmed/36527507
http://dx.doi.org/10.1007/s00775-022-01972-1

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