Preparation of chaperone-loaded neural stem cell-derived extracellular vesicles to reduce protein aggregation in Huntington’s disease cellular models

Here, we present a protocol using genetic engineering techniques to prepare small extracellular vesicles (sEVs) enriched in the chaperone protein DNAJB6. We describe steps to prepare cell lines overexpressing DNAJB6, followed by the isolation and characterization of sEVs from cell conditioned media....

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Detalles Bibliográficos
Autores principales: Joshi, Bhagyashree S., Zuhorn, Inge S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9985028/
https://www.ncbi.nlm.nih.gov/pubmed/36861837
http://dx.doi.org/10.1016/j.xpro.2023.102134

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9985028/
https://www.ncbi.nlm.nih.gov/pubmed/36861837
http://dx.doi.org/10.1016/j.xpro.2023.102134

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