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Mapping protein direct interactome of oxidoreductases with small molecular chemical cross-linkers in live cells

Identifying direct substrates of enzymes has been a long-term challenge. Here, we present a strategy using live cell chemical cross-linking and mass spectrometry to identify the putative substrates of enzymes for further biochemical validation. Compared with other methods, our strategy is based on t...

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Detalles Bibliográficos
Autores principales: Wu, Ting, Li, Shang-Tong, Ran, Yu, Lin, Yinuo, Liu, Lu, Zhang, Xiajun, Zhou, Lianqi, Zhang, Long, Wu, Donghai, Yang, Bing, Tang, Shibing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9986639/
https://www.ncbi.nlm.nih.gov/pubmed/36863169
http://dx.doi.org/10.1016/j.redox.2023.102642