Evaluate the guide RNA effectiveness via Agrobacterium-mediated transient assays in Nicotiana benthamiana

CRISPR/Cas9-based genome editing system is a powerful tool for plant genetic improvement. However, the variable efficiency of guide RNA(s) (gRNA) represents a key limiting factor that hampers the broad application of the CRISPR/Cas9 system in crop improvement. Here, we employed the Agrobacterium-med...

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Detalles Bibliográficos
Autores principales: Wang, Zhibo, Shea, Zachary, Li, Qi, Wang, Kunru, Mills, Kerri, Zhang, Bo, Zhao, Bingyu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Plant Science
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9986745/
https://www.ncbi.nlm.nih.gov/pubmed/36890894
http://dx.doi.org/10.3389/fpls.2023.1111683

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9986745/
https://www.ncbi.nlm.nih.gov/pubmed/36890894
http://dx.doi.org/10.3389/fpls.2023.1111683

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