Characterization of bone marrow heterogeneity in NK-AML (M4/M5) based on single-cell RNA sequencing

Normal karyotype acute myeloid leukemia (NK-AML) is a heterogeneous hematological malignancy that contains a minor population of self-renewing leukemia stem cells (LSCs), which complicate efforts to achieve long-term survival. We performed single-cell RNA sequencing to profile 39,288 cells from 6 bo...

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Autores principales: Wu, Wenqi, Shi, Zeyan, Tang, Zhongyuan, Li, Huiqun, Huang, Xiaoke, Liang, Xiaolin, Li, Jing, Yao, Yibin, Zhao, Weihua, Wu, Meiqing, Luo, Jun, Liu, Zhenfang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2023
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9987113/
https://www.ncbi.nlm.nih.gov/pubmed/36879313
http://dx.doi.org/10.1186/s40164-023-00391-5
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author Wu, Wenqi
Shi, Zeyan
Tang, Zhongyuan
Li, Huiqun
Huang, Xiaoke
Liang, Xiaolin
Li, Jing
Yao, Yibin
Zhao, Weihua
Wu, Meiqing
Luo, Jun
Liu, Zhenfang
author_facet Wu, Wenqi
Shi, Zeyan
Tang, Zhongyuan
Li, Huiqun
Huang, Xiaoke
Liang, Xiaolin
Li, Jing
Yao, Yibin
Zhao, Weihua
Wu, Meiqing
Luo, Jun
Liu, Zhenfang
author_sort Wu, Wenqi
collection PubMed
description Normal karyotype acute myeloid leukemia (NK-AML) is a heterogeneous hematological malignancy that contains a minor population of self-renewing leukemia stem cells (LSCs), which complicate efforts to achieve long-term survival. We performed single-cell RNA sequencing to profile 39,288 cells from 6 bone marrow (BM) aspirates including 5 NK-AML (M4/M5) patients and 1 healthy donor. The single-cell transcriptome atlas and gene expression characteristics of each cell population in NK-AML (M4/M5) and healthy BM were obtained. In addition, we identified a distinct LSC-like cluster with possible biomarkers in NK-AML (M4/M5) and verified 6 genes using qRT‒PCR and bioinformatic analyses. In conclusion, we utilized single-cell technologies to provide an atlas of NK-AML (M4/M5) cell heterogeneity, composition, and biomarkers with implications for precision medicine and targeted therapies. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s40164-023-00391-5.
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spelling pubmed-99871132023-03-07 Characterization of bone marrow heterogeneity in NK-AML (M4/M5) based on single-cell RNA sequencing Wu, Wenqi Shi, Zeyan Tang, Zhongyuan Li, Huiqun Huang, Xiaoke Liang, Xiaolin Li, Jing Yao, Yibin Zhao, Weihua Wu, Meiqing Luo, Jun Liu, Zhenfang Exp Hematol Oncol Research Normal karyotype acute myeloid leukemia (NK-AML) is a heterogeneous hematological malignancy that contains a minor population of self-renewing leukemia stem cells (LSCs), which complicate efforts to achieve long-term survival. We performed single-cell RNA sequencing to profile 39,288 cells from 6 bone marrow (BM) aspirates including 5 NK-AML (M4/M5) patients and 1 healthy donor. The single-cell transcriptome atlas and gene expression characteristics of each cell population in NK-AML (M4/M5) and healthy BM were obtained. In addition, we identified a distinct LSC-like cluster with possible biomarkers in NK-AML (M4/M5) and verified 6 genes using qRT‒PCR and bioinformatic analyses. In conclusion, we utilized single-cell technologies to provide an atlas of NK-AML (M4/M5) cell heterogeneity, composition, and biomarkers with implications for precision medicine and targeted therapies. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s40164-023-00391-5. BioMed Central 2023-03-06 /pmc/articles/PMC9987113/ /pubmed/36879313 http://dx.doi.org/10.1186/s40164-023-00391-5 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Wu, Wenqi
Shi, Zeyan
Tang, Zhongyuan
Li, Huiqun
Huang, Xiaoke
Liang, Xiaolin
Li, Jing
Yao, Yibin
Zhao, Weihua
Wu, Meiqing
Luo, Jun
Liu, Zhenfang
Characterization of bone marrow heterogeneity in NK-AML (M4/M5) based on single-cell RNA sequencing
title Characterization of bone marrow heterogeneity in NK-AML (M4/M5) based on single-cell RNA sequencing
title_full Characterization of bone marrow heterogeneity in NK-AML (M4/M5) based on single-cell RNA sequencing
title_fullStr Characterization of bone marrow heterogeneity in NK-AML (M4/M5) based on single-cell RNA sequencing
title_full_unstemmed Characterization of bone marrow heterogeneity in NK-AML (M4/M5) based on single-cell RNA sequencing
title_short Characterization of bone marrow heterogeneity in NK-AML (M4/M5) based on single-cell RNA sequencing
title_sort characterization of bone marrow heterogeneity in nk-aml (m4/m5) based on single-cell rna sequencing
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9987113/
https://www.ncbi.nlm.nih.gov/pubmed/36879313
http://dx.doi.org/10.1186/s40164-023-00391-5
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