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Efficient knock-in method enabling lineage tracing in zebrafish
Here, we devised a cloning-free 3′ knock-in strategy for zebrafish using PCR amplified dsDNA donors that avoids disrupting the targeted genes. The dsDNA donors carry genetic cassettes coding for fluorescent proteins and Cre recombinase in frame with the endogenous gene but separated from it by self-...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Life Science Alliance LLC
2023
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9990459/ https://www.ncbi.nlm.nih.gov/pubmed/36878640 http://dx.doi.org/10.26508/lsa.202301944 |