Optimized Expression and Isolation of Recombinant Active Secreted Proteases Using Pichia pastoris

Recombinant proteins of high quality are crucial starting materials for all downstream applications, but the inherent complexities of proteins and their expression and purification create significant challenges. The Pichia pastoris yeast is a highly useful eukaryotic protein expression system. Pichi...

Descripción completa

Detalles Bibliográficos
Autores principales: Lanser, Dylan M., Gelli, Angie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Bio-Protocol 2023
Materias:
Methods Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9993078/
https://www.ncbi.nlm.nih.gov/pubmed/36908634
http://dx.doi.org/10.21769/BioProtoc.4628

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9993078/
https://www.ncbi.nlm.nih.gov/pubmed/36908634
http://dx.doi.org/10.21769/BioProtoc.4628

Ejemplares similares