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Optimized Expression and Isolation of Recombinant Active Secreted Proteases Using Pichia pastoris
Recombinant proteins of high quality are crucial starting materials for all downstream applications, but the inherent complexities of proteins and their expression and purification create significant challenges. The Pichia pastoris yeast is a highly useful eukaryotic protein expression system. Pichi...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Bio-Protocol
2023
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9993078/ https://www.ncbi.nlm.nih.gov/pubmed/36908634 http://dx.doi.org/10.21769/BioProtoc.4628 |