The integrated analysis of digestive physiology and gastrointestinal microbiota structure in Changle goose

Changle goose in Fujian, China is a rare genetic resource and in urgent need to be protected. Understanding the characteristics of digestive physiology and spatial variation of gastrointestinal microbiota is crucial for developing nutritional intervention strategies to improve intestinal health and...

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Autores principales: Fang, Shaoming, Liu, Jing, Wei, Suhong, Yang, Guofeng, Chen, Xinzhu, Tong, Yuxin, Guo, Pingting
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10031540/
https://www.ncbi.nlm.nih.gov/pubmed/36933526
http://dx.doi.org/10.1016/j.psj.2023.102588
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author Fang, Shaoming
Liu, Jing
Wei, Suhong
Yang, Guofeng
Chen, Xinzhu
Tong, Yuxin
Guo, Pingting
author_facet Fang, Shaoming
Liu, Jing
Wei, Suhong
Yang, Guofeng
Chen, Xinzhu
Tong, Yuxin
Guo, Pingting
author_sort Fang, Shaoming
collection PubMed
description Changle goose in Fujian, China is a rare genetic resource and in urgent need to be protected. Understanding the characteristics of digestive physiology and spatial variation of gastrointestinal microbiota is crucial for developing nutritional intervention strategies to improve intestinal health and production performance of goose. Hence, histomorphological assay was used for observing development status of proventriculus, jejunum, and cecum in 70-day-old Changle geese, whereas digesta from 6 alimentary canal locations (crop, proventriculus, gizzard, jejunum, cecum, and rectum) were collected for 16S rRNA gene sequencing and short chain fatty acids (SCFAs) quantitative analysis. The histomorphological observation indicated that the jejunum and cecum of Changle goose were well developed. The alpha diversity analysis revealed that, except rectum, microbiota in other noncecum sections were in high diversity as cecum. The Nonmetric MultiDimensional Scaling (NMDS) analysis showed that microbial community of proventriculus, gizzard, and jejunum formed a cluster, which distinctly discrete with the microbiota of the other gastrointestinal locations. Additionally, the proportions of Proteobacteria, Bacteroidota, and Campilobacterota at the phylum level and Lactobacillus, Streptococcus, Helicobacter, and Subdoligranulum at the genus level exhibited tremendous alternations among different gastrointestinal locations. The characteristic bacterial composition in each section was further disclosed by analyzing the core and feature Amplicon Sequence Variants (ASVs) and SCFAs pattern. Importantly, 7 body-weight-associated ASVs and 2 cecum-development-related ASVs were identified via correlation analysis. In a whole, our findings provided the first insights into the specialized digestive physiology of Changle geese and distinctive regional distribution of gastrointestinal microbiota, which laid the important foundation for improving growth performance through microbiota manipulation in geese.
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spelling pubmed-100315402023-03-23 The integrated analysis of digestive physiology and gastrointestinal microbiota structure in Changle goose Fang, Shaoming Liu, Jing Wei, Suhong Yang, Guofeng Chen, Xinzhu Tong, Yuxin Guo, Pingting Poult Sci MICROBIOLOGY AND FOOD SAFETY Changle goose in Fujian, China is a rare genetic resource and in urgent need to be protected. Understanding the characteristics of digestive physiology and spatial variation of gastrointestinal microbiota is crucial for developing nutritional intervention strategies to improve intestinal health and production performance of goose. Hence, histomorphological assay was used for observing development status of proventriculus, jejunum, and cecum in 70-day-old Changle geese, whereas digesta from 6 alimentary canal locations (crop, proventriculus, gizzard, jejunum, cecum, and rectum) were collected for 16S rRNA gene sequencing and short chain fatty acids (SCFAs) quantitative analysis. The histomorphological observation indicated that the jejunum and cecum of Changle goose were well developed. The alpha diversity analysis revealed that, except rectum, microbiota in other noncecum sections were in high diversity as cecum. The Nonmetric MultiDimensional Scaling (NMDS) analysis showed that microbial community of proventriculus, gizzard, and jejunum formed a cluster, which distinctly discrete with the microbiota of the other gastrointestinal locations. Additionally, the proportions of Proteobacteria, Bacteroidota, and Campilobacterota at the phylum level and Lactobacillus, Streptococcus, Helicobacter, and Subdoligranulum at the genus level exhibited tremendous alternations among different gastrointestinal locations. The characteristic bacterial composition in each section was further disclosed by analyzing the core and feature Amplicon Sequence Variants (ASVs) and SCFAs pattern. Importantly, 7 body-weight-associated ASVs and 2 cecum-development-related ASVs were identified via correlation analysis. In a whole, our findings provided the first insights into the specialized digestive physiology of Changle geese and distinctive regional distribution of gastrointestinal microbiota, which laid the important foundation for improving growth performance through microbiota manipulation in geese. Elsevier 2023-02-15 /pmc/articles/PMC10031540/ /pubmed/36933526 http://dx.doi.org/10.1016/j.psj.2023.102588 Text en © 2023 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle MICROBIOLOGY AND FOOD SAFETY
Fang, Shaoming
Liu, Jing
Wei, Suhong
Yang, Guofeng
Chen, Xinzhu
Tong, Yuxin
Guo, Pingting
The integrated analysis of digestive physiology and gastrointestinal microbiota structure in Changle goose
title The integrated analysis of digestive physiology and gastrointestinal microbiota structure in Changle goose
title_full The integrated analysis of digestive physiology and gastrointestinal microbiota structure in Changle goose
title_fullStr The integrated analysis of digestive physiology and gastrointestinal microbiota structure in Changle goose
title_full_unstemmed The integrated analysis of digestive physiology and gastrointestinal microbiota structure in Changle goose
title_short The integrated analysis of digestive physiology and gastrointestinal microbiota structure in Changle goose
title_sort integrated analysis of digestive physiology and gastrointestinal microbiota structure in changle goose
topic MICROBIOLOGY AND FOOD SAFETY
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10031540/
https://www.ncbi.nlm.nih.gov/pubmed/36933526
http://dx.doi.org/10.1016/j.psj.2023.102588
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