The integrated analysis of digestive physiology and gastrointestinal microbiota structure in Changle goose
Changle goose in Fujian, China is a rare genetic resource and in urgent need to be protected. Understanding the characteristics of digestive physiology and spatial variation of gastrointestinal microbiota is crucial for developing nutritional intervention strategies to improve intestinal health and...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10031540/ https://www.ncbi.nlm.nih.gov/pubmed/36933526 http://dx.doi.org/10.1016/j.psj.2023.102588 |
_version_ | 1784910626981675008 |
---|---|
author | Fang, Shaoming Liu, Jing Wei, Suhong Yang, Guofeng Chen, Xinzhu Tong, Yuxin Guo, Pingting |
author_facet | Fang, Shaoming Liu, Jing Wei, Suhong Yang, Guofeng Chen, Xinzhu Tong, Yuxin Guo, Pingting |
author_sort | Fang, Shaoming |
collection | PubMed |
description | Changle goose in Fujian, China is a rare genetic resource and in urgent need to be protected. Understanding the characteristics of digestive physiology and spatial variation of gastrointestinal microbiota is crucial for developing nutritional intervention strategies to improve intestinal health and production performance of goose. Hence, histomorphological assay was used for observing development status of proventriculus, jejunum, and cecum in 70-day-old Changle geese, whereas digesta from 6 alimentary canal locations (crop, proventriculus, gizzard, jejunum, cecum, and rectum) were collected for 16S rRNA gene sequencing and short chain fatty acids (SCFAs) quantitative analysis. The histomorphological observation indicated that the jejunum and cecum of Changle goose were well developed. The alpha diversity analysis revealed that, except rectum, microbiota in other noncecum sections were in high diversity as cecum. The Nonmetric MultiDimensional Scaling (NMDS) analysis showed that microbial community of proventriculus, gizzard, and jejunum formed a cluster, which distinctly discrete with the microbiota of the other gastrointestinal locations. Additionally, the proportions of Proteobacteria, Bacteroidota, and Campilobacterota at the phylum level and Lactobacillus, Streptococcus, Helicobacter, and Subdoligranulum at the genus level exhibited tremendous alternations among different gastrointestinal locations. The characteristic bacterial composition in each section was further disclosed by analyzing the core and feature Amplicon Sequence Variants (ASVs) and SCFAs pattern. Importantly, 7 body-weight-associated ASVs and 2 cecum-development-related ASVs were identified via correlation analysis. In a whole, our findings provided the first insights into the specialized digestive physiology of Changle geese and distinctive regional distribution of gastrointestinal microbiota, which laid the important foundation for improving growth performance through microbiota manipulation in geese. |
format | Online Article Text |
id | pubmed-10031540 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-100315402023-03-23 The integrated analysis of digestive physiology and gastrointestinal microbiota structure in Changle goose Fang, Shaoming Liu, Jing Wei, Suhong Yang, Guofeng Chen, Xinzhu Tong, Yuxin Guo, Pingting Poult Sci MICROBIOLOGY AND FOOD SAFETY Changle goose in Fujian, China is a rare genetic resource and in urgent need to be protected. Understanding the characteristics of digestive physiology and spatial variation of gastrointestinal microbiota is crucial for developing nutritional intervention strategies to improve intestinal health and production performance of goose. Hence, histomorphological assay was used for observing development status of proventriculus, jejunum, and cecum in 70-day-old Changle geese, whereas digesta from 6 alimentary canal locations (crop, proventriculus, gizzard, jejunum, cecum, and rectum) were collected for 16S rRNA gene sequencing and short chain fatty acids (SCFAs) quantitative analysis. The histomorphological observation indicated that the jejunum and cecum of Changle goose were well developed. The alpha diversity analysis revealed that, except rectum, microbiota in other noncecum sections were in high diversity as cecum. The Nonmetric MultiDimensional Scaling (NMDS) analysis showed that microbial community of proventriculus, gizzard, and jejunum formed a cluster, which distinctly discrete with the microbiota of the other gastrointestinal locations. Additionally, the proportions of Proteobacteria, Bacteroidota, and Campilobacterota at the phylum level and Lactobacillus, Streptococcus, Helicobacter, and Subdoligranulum at the genus level exhibited tremendous alternations among different gastrointestinal locations. The characteristic bacterial composition in each section was further disclosed by analyzing the core and feature Amplicon Sequence Variants (ASVs) and SCFAs pattern. Importantly, 7 body-weight-associated ASVs and 2 cecum-development-related ASVs were identified via correlation analysis. In a whole, our findings provided the first insights into the specialized digestive physiology of Changle geese and distinctive regional distribution of gastrointestinal microbiota, which laid the important foundation for improving growth performance through microbiota manipulation in geese. Elsevier 2023-02-15 /pmc/articles/PMC10031540/ /pubmed/36933526 http://dx.doi.org/10.1016/j.psj.2023.102588 Text en © 2023 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | MICROBIOLOGY AND FOOD SAFETY Fang, Shaoming Liu, Jing Wei, Suhong Yang, Guofeng Chen, Xinzhu Tong, Yuxin Guo, Pingting The integrated analysis of digestive physiology and gastrointestinal microbiota structure in Changle goose |
title | The integrated analysis of digestive physiology and gastrointestinal microbiota structure in Changle goose |
title_full | The integrated analysis of digestive physiology and gastrointestinal microbiota structure in Changle goose |
title_fullStr | The integrated analysis of digestive physiology and gastrointestinal microbiota structure in Changle goose |
title_full_unstemmed | The integrated analysis of digestive physiology and gastrointestinal microbiota structure in Changle goose |
title_short | The integrated analysis of digestive physiology and gastrointestinal microbiota structure in Changle goose |
title_sort | integrated analysis of digestive physiology and gastrointestinal microbiota structure in changle goose |
topic | MICROBIOLOGY AND FOOD SAFETY |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10031540/ https://www.ncbi.nlm.nih.gov/pubmed/36933526 http://dx.doi.org/10.1016/j.psj.2023.102588 |
work_keys_str_mv | AT fangshaoming theintegratedanalysisofdigestivephysiologyandgastrointestinalmicrobiotastructureinchanglegoose AT liujing theintegratedanalysisofdigestivephysiologyandgastrointestinalmicrobiotastructureinchanglegoose AT weisuhong theintegratedanalysisofdigestivephysiologyandgastrointestinalmicrobiotastructureinchanglegoose AT yangguofeng theintegratedanalysisofdigestivephysiologyandgastrointestinalmicrobiotastructureinchanglegoose AT chenxinzhu theintegratedanalysisofdigestivephysiologyandgastrointestinalmicrobiotastructureinchanglegoose AT tongyuxin theintegratedanalysisofdigestivephysiologyandgastrointestinalmicrobiotastructureinchanglegoose AT guopingting theintegratedanalysisofdigestivephysiologyandgastrointestinalmicrobiotastructureinchanglegoose AT fangshaoming integratedanalysisofdigestivephysiologyandgastrointestinalmicrobiotastructureinchanglegoose AT liujing integratedanalysisofdigestivephysiologyandgastrointestinalmicrobiotastructureinchanglegoose AT weisuhong integratedanalysisofdigestivephysiologyandgastrointestinalmicrobiotastructureinchanglegoose AT yangguofeng integratedanalysisofdigestivephysiologyandgastrointestinalmicrobiotastructureinchanglegoose AT chenxinzhu integratedanalysisofdigestivephysiologyandgastrointestinalmicrobiotastructureinchanglegoose AT tongyuxin integratedanalysisofdigestivephysiologyandgastrointestinalmicrobiotastructureinchanglegoose AT guopingting integratedanalysisofdigestivephysiologyandgastrointestinalmicrobiotastructureinchanglegoose |