Analysis of hemopexin plasma levels in patients with age-related macular degeneration
PURPOSE: A protein quantitative trait locus (pQTL) analysis recently revealed a strong association between hemopexin (HPX) levels and genetic variants at the complement factor H (CFH) locus. In this study, we aimed to determine HPX plasma levels in patients with age-related macular degeneration (AMD...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Molecular Vision
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10115365/ https://www.ncbi.nlm.nih.gov/pubmed/37089696 |
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author | Lauwen, Susette Bakker, Bjorn de Jong, Eiko K. Fauser, Sascha Hoyng, Carel B. Lefeber, Dirk J. den Hollander, Anneke I. |
author_facet | Lauwen, Susette Bakker, Bjorn de Jong, Eiko K. Fauser, Sascha Hoyng, Carel B. Lefeber, Dirk J. den Hollander, Anneke I. |
author_sort | Lauwen, Susette |
collection | PubMed |
description | PURPOSE: A protein quantitative trait locus (pQTL) analysis recently revealed a strong association between hemopexin (HPX) levels and genetic variants at the complement factor H (CFH) locus. In this study, we aimed to determine HPX plasma levels in patients with age-related macular degeneration (AMD) and to compare them with those in controls. We also investigated whether genetic variants at the CFH locus are associated with HPX plasma levels. METHODS: HPX levels were quantified in 200 advanced AMD cases and 200 controls using an enzyme-linked immunosorbent assay and compared between the two groups. Furthermore, HPX levels were analyzed per genotype group of three HPX-associated variants (rs61818956, rs10494745, and rs10801582) and four AMD-associated variants (rs794362 [proxy for rs187328863], rs570618, rs10922109, and rs61818924 [proxy for rs61818925]) at the CFH locus. RESULTS: HPX levels were similar in the control group compared with the AMD group. The three variants at the CFH locus, which were previously associated with the HPX levels, showed no association with the HPX levels in our data set. No significant differences in HPX levels were detected between the different genotype groups of AMD-associated variants at the CFH locus. CONCLUSIONS: In this study, HPX levels were not associated with AMD or AMD-associated variants at the CFH locus. The finding of a previous pQTL study that variants at the CFH locus were associated with HPX levels was also not confirmed in this study. |
format | Online Article Text |
id | pubmed-10115365 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Molecular Vision |
record_format | MEDLINE/PubMed |
spelling | pubmed-101153652023-04-20 Analysis of hemopexin plasma levels in patients with age-related macular degeneration Lauwen, Susette Bakker, Bjorn de Jong, Eiko K. Fauser, Sascha Hoyng, Carel B. Lefeber, Dirk J. den Hollander, Anneke I. Mol Vis Research Article PURPOSE: A protein quantitative trait locus (pQTL) analysis recently revealed a strong association between hemopexin (HPX) levels and genetic variants at the complement factor H (CFH) locus. In this study, we aimed to determine HPX plasma levels in patients with age-related macular degeneration (AMD) and to compare them with those in controls. We also investigated whether genetic variants at the CFH locus are associated with HPX plasma levels. METHODS: HPX levels were quantified in 200 advanced AMD cases and 200 controls using an enzyme-linked immunosorbent assay and compared between the two groups. Furthermore, HPX levels were analyzed per genotype group of three HPX-associated variants (rs61818956, rs10494745, and rs10801582) and four AMD-associated variants (rs794362 [proxy for rs187328863], rs570618, rs10922109, and rs61818924 [proxy for rs61818925]) at the CFH locus. RESULTS: HPX levels were similar in the control group compared with the AMD group. The three variants at the CFH locus, which were previously associated with the HPX levels, showed no association with the HPX levels in our data set. No significant differences in HPX levels were detected between the different genotype groups of AMD-associated variants at the CFH locus. CONCLUSIONS: In this study, HPX levels were not associated with AMD or AMD-associated variants at the CFH locus. The finding of a previous pQTL study that variants at the CFH locus were associated with HPX levels was also not confirmed in this study. Molecular Vision 2022-12-31 /pmc/articles/PMC10115365/ /pubmed/37089696 Text en Copyright © 2022 Molecular Vision. https://creativecommons.org/licenses/by-nc-nd/3.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited, used for non-commercial purposes, and is not altered or transformed. |
spellingShingle | Research Article Lauwen, Susette Bakker, Bjorn de Jong, Eiko K. Fauser, Sascha Hoyng, Carel B. Lefeber, Dirk J. den Hollander, Anneke I. Analysis of hemopexin plasma levels in patients with age-related macular degeneration |
title | Analysis of hemopexin plasma levels in patients with age-related macular degeneration |
title_full | Analysis of hemopexin plasma levels in patients with age-related macular degeneration |
title_fullStr | Analysis of hemopexin plasma levels in patients with age-related macular degeneration |
title_full_unstemmed | Analysis of hemopexin plasma levels in patients with age-related macular degeneration |
title_short | Analysis of hemopexin plasma levels in patients with age-related macular degeneration |
title_sort | analysis of hemopexin plasma levels in patients with age-related macular degeneration |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10115365/ https://www.ncbi.nlm.nih.gov/pubmed/37089696 |
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