Purification of primary human placental leukocytes to study maternal-fetal interactions

Decidual leukocytes play key roles in maternal-fetal tolerance and immunity. Here, we present detailed methods to purify, culture, and functionally analyze human placental dNK, dTreg, dTem, and dMɸ from decidua parietalis, the maternal part of the placental membranes; decidua basalis, the maternal p...

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Autores principales: Ikumi, Nadia M., Koenig, Zachary, Mahajan, Shweta, Gray, Clive M., Tilburgs, Tamara
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10199256/
https://www.ncbi.nlm.nih.gov/pubmed/37182206
http://dx.doi.org/10.1016/j.xpro.2023.102277
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author Ikumi, Nadia M.
Koenig, Zachary
Mahajan, Shweta
Gray, Clive M.
Tilburgs, Tamara
author_facet Ikumi, Nadia M.
Koenig, Zachary
Mahajan, Shweta
Gray, Clive M.
Tilburgs, Tamara
author_sort Ikumi, Nadia M.
collection PubMed
description Decidual leukocytes play key roles in maternal-fetal tolerance and immunity. Here, we present detailed methods to purify, culture, and functionally analyze human placental dNK, dTreg, dTem, and dMɸ from decidua parietalis, the maternal part of the placental membranes; decidua basalis, the maternal part of the placenta; and placental villi. These sites have high clinical relevance in the development of villitis and chorioamnionitis. This allows in-depth phenotypic and functional investigation of placental immune populations and their interactions with extravillous trophoblasts. For complete details on the use and execution of this protocol, please refer to Ikumi et al.,(1) Tilburgs et al.,(2) Salvany-Celades et al.,(3) Crespo et al.,(4) van der Zwan et al.(5)
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spelling pubmed-101992562023-05-21 Purification of primary human placental leukocytes to study maternal-fetal interactions Ikumi, Nadia M. Koenig, Zachary Mahajan, Shweta Gray, Clive M. Tilburgs, Tamara STAR Protoc Protocol Decidual leukocytes play key roles in maternal-fetal tolerance and immunity. Here, we present detailed methods to purify, culture, and functionally analyze human placental dNK, dTreg, dTem, and dMɸ from decidua parietalis, the maternal part of the placental membranes; decidua basalis, the maternal part of the placenta; and placental villi. These sites have high clinical relevance in the development of villitis and chorioamnionitis. This allows in-depth phenotypic and functional investigation of placental immune populations and their interactions with extravillous trophoblasts. For complete details on the use and execution of this protocol, please refer to Ikumi et al.,(1) Tilburgs et al.,(2) Salvany-Celades et al.,(3) Crespo et al.,(4) van der Zwan et al.(5) Elsevier 2023-05-12 /pmc/articles/PMC10199256/ /pubmed/37182206 http://dx.doi.org/10.1016/j.xpro.2023.102277 Text en © 2023 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Ikumi, Nadia M.
Koenig, Zachary
Mahajan, Shweta
Gray, Clive M.
Tilburgs, Tamara
Purification of primary human placental leukocytes to study maternal-fetal interactions
title Purification of primary human placental leukocytes to study maternal-fetal interactions
title_full Purification of primary human placental leukocytes to study maternal-fetal interactions
title_fullStr Purification of primary human placental leukocytes to study maternal-fetal interactions
title_full_unstemmed Purification of primary human placental leukocytes to study maternal-fetal interactions
title_short Purification of primary human placental leukocytes to study maternal-fetal interactions
title_sort purification of primary human placental leukocytes to study maternal-fetal interactions
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10199256/
https://www.ncbi.nlm.nih.gov/pubmed/37182206
http://dx.doi.org/10.1016/j.xpro.2023.102277
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