Purification of primary human placental leukocytes to study maternal-fetal interactions
Decidual leukocytes play key roles in maternal-fetal tolerance and immunity. Here, we present detailed methods to purify, culture, and functionally analyze human placental dNK, dTreg, dTem, and dMɸ from decidua parietalis, the maternal part of the placental membranes; decidua basalis, the maternal p...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10199256/ https://www.ncbi.nlm.nih.gov/pubmed/37182206 http://dx.doi.org/10.1016/j.xpro.2023.102277 |
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author | Ikumi, Nadia M. Koenig, Zachary Mahajan, Shweta Gray, Clive M. Tilburgs, Tamara |
author_facet | Ikumi, Nadia M. Koenig, Zachary Mahajan, Shweta Gray, Clive M. Tilburgs, Tamara |
author_sort | Ikumi, Nadia M. |
collection | PubMed |
description | Decidual leukocytes play key roles in maternal-fetal tolerance and immunity. Here, we present detailed methods to purify, culture, and functionally analyze human placental dNK, dTreg, dTem, and dMɸ from decidua parietalis, the maternal part of the placental membranes; decidua basalis, the maternal part of the placenta; and placental villi. These sites have high clinical relevance in the development of villitis and chorioamnionitis. This allows in-depth phenotypic and functional investigation of placental immune populations and their interactions with extravillous trophoblasts. For complete details on the use and execution of this protocol, please refer to Ikumi et al.,(1) Tilburgs et al.,(2) Salvany-Celades et al.,(3) Crespo et al.,(4) van der Zwan et al.(5) |
format | Online Article Text |
id | pubmed-10199256 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-101992562023-05-21 Purification of primary human placental leukocytes to study maternal-fetal interactions Ikumi, Nadia M. Koenig, Zachary Mahajan, Shweta Gray, Clive M. Tilburgs, Tamara STAR Protoc Protocol Decidual leukocytes play key roles in maternal-fetal tolerance and immunity. Here, we present detailed methods to purify, culture, and functionally analyze human placental dNK, dTreg, dTem, and dMɸ from decidua parietalis, the maternal part of the placental membranes; decidua basalis, the maternal part of the placenta; and placental villi. These sites have high clinical relevance in the development of villitis and chorioamnionitis. This allows in-depth phenotypic and functional investigation of placental immune populations and their interactions with extravillous trophoblasts. For complete details on the use and execution of this protocol, please refer to Ikumi et al.,(1) Tilburgs et al.,(2) Salvany-Celades et al.,(3) Crespo et al.,(4) van der Zwan et al.(5) Elsevier 2023-05-12 /pmc/articles/PMC10199256/ /pubmed/37182206 http://dx.doi.org/10.1016/j.xpro.2023.102277 Text en © 2023 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Protocol Ikumi, Nadia M. Koenig, Zachary Mahajan, Shweta Gray, Clive M. Tilburgs, Tamara Purification of primary human placental leukocytes to study maternal-fetal interactions |
title | Purification of primary human placental leukocytes to study maternal-fetal interactions |
title_full | Purification of primary human placental leukocytes to study maternal-fetal interactions |
title_fullStr | Purification of primary human placental leukocytes to study maternal-fetal interactions |
title_full_unstemmed | Purification of primary human placental leukocytes to study maternal-fetal interactions |
title_short | Purification of primary human placental leukocytes to study maternal-fetal interactions |
title_sort | purification of primary human placental leukocytes to study maternal-fetal interactions |
topic | Protocol |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10199256/ https://www.ncbi.nlm.nih.gov/pubmed/37182206 http://dx.doi.org/10.1016/j.xpro.2023.102277 |
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