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Derivatives of Differentiation-Inducing Factor 1 Differentially Control Chemotaxis and Stalk Cell Differentiation in Dictyostelium discoideum
SIMPLE SUMMARY: In this study, we examined the effects of nine DIF derivatives on chemotactic cell movement toward cAMP and compared their chemotaxis-modulating activity and stalk cell differentiation–inducing activity in wild-type and mutant strains in Dictyostelium discoideum. We found that the DI...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10295651/ https://www.ncbi.nlm.nih.gov/pubmed/37372157 http://dx.doi.org/10.3390/biology12060873 |
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author | Kuwayama, Hidekazu Kikuchi, Haruhisa Kubohara, Yuzuru |
author_facet | Kuwayama, Hidekazu Kikuchi, Haruhisa Kubohara, Yuzuru |
author_sort | Kuwayama, Hidekazu |
collection | PubMed |
description | SIMPLE SUMMARY: In this study, we examined the effects of nine DIF derivatives on chemotactic cell movement toward cAMP and compared their chemotaxis-modulating activity and stalk cell differentiation–inducing activity in wild-type and mutant strains in Dictyostelium discoideum. We found that the DIF derivatives differentially affected chemotaxis and stalk cell differentiation, suggesting that DIF-1 and DIF-2 have at least three receptors: one for stalk cell induction and two for chemotaxis modulation. ABSTRACT: Differentiation-inducing factors 1 and 2 (DIF-1 and DIF-2) are small lipophilic signal molecules that induce stalk cell differentiation but differentially modulate chemotaxis toward cAMP in the cellular slime mold Dictyostelium discoideum; DIF-1 suppresses chemotactic cell movement in shallow cAMP gradients, whereas DIF-2 promotes it. The receptor(s) for DIF-1 and DIF-2 have not yet been identified. We examined the effects of nine derivatives of DIF-1 on chemotactic cell movement toward cAMP and compared their chemotaxis-modulating activity and stalk cell differentiation–inducing activity in wild-type and mutant strains. The DIF derivatives differentially affected chemotaxis and stalk cell differentiation; for example, TM-DIF-1 suppressed chemotaxis and showed poor stalk-inducing activity, DIF-1(3M) suppressed chemotaxis and showed strong stalk-inducing activity, and TH-DIF-1 promoted chemotaxis. These results suggest that DIF-1 and DIF-2 have at least three receptors: one for stalk cell induction and two for chemotaxis modulation. In addition, our results show that the DIF derivatives can be used to analyze the DIF-signaling pathways in D. discoideum. |
format | Online Article Text |
id | pubmed-10295651 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-102956512023-06-28 Derivatives of Differentiation-Inducing Factor 1 Differentially Control Chemotaxis and Stalk Cell Differentiation in Dictyostelium discoideum Kuwayama, Hidekazu Kikuchi, Haruhisa Kubohara, Yuzuru Biology (Basel) Article SIMPLE SUMMARY: In this study, we examined the effects of nine DIF derivatives on chemotactic cell movement toward cAMP and compared their chemotaxis-modulating activity and stalk cell differentiation–inducing activity in wild-type and mutant strains in Dictyostelium discoideum. We found that the DIF derivatives differentially affected chemotaxis and stalk cell differentiation, suggesting that DIF-1 and DIF-2 have at least three receptors: one for stalk cell induction and two for chemotaxis modulation. ABSTRACT: Differentiation-inducing factors 1 and 2 (DIF-1 and DIF-2) are small lipophilic signal molecules that induce stalk cell differentiation but differentially modulate chemotaxis toward cAMP in the cellular slime mold Dictyostelium discoideum; DIF-1 suppresses chemotactic cell movement in shallow cAMP gradients, whereas DIF-2 promotes it. The receptor(s) for DIF-1 and DIF-2 have not yet been identified. We examined the effects of nine derivatives of DIF-1 on chemotactic cell movement toward cAMP and compared their chemotaxis-modulating activity and stalk cell differentiation–inducing activity in wild-type and mutant strains. The DIF derivatives differentially affected chemotaxis and stalk cell differentiation; for example, TM-DIF-1 suppressed chemotaxis and showed poor stalk-inducing activity, DIF-1(3M) suppressed chemotaxis and showed strong stalk-inducing activity, and TH-DIF-1 promoted chemotaxis. These results suggest that DIF-1 and DIF-2 have at least three receptors: one for stalk cell induction and two for chemotaxis modulation. In addition, our results show that the DIF derivatives can be used to analyze the DIF-signaling pathways in D. discoideum. MDPI 2023-06-16 /pmc/articles/PMC10295651/ /pubmed/37372157 http://dx.doi.org/10.3390/biology12060873 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Kuwayama, Hidekazu Kikuchi, Haruhisa Kubohara, Yuzuru Derivatives of Differentiation-Inducing Factor 1 Differentially Control Chemotaxis and Stalk Cell Differentiation in Dictyostelium discoideum |
title | Derivatives of Differentiation-Inducing Factor 1 Differentially Control Chemotaxis and Stalk Cell Differentiation in Dictyostelium discoideum |
title_full | Derivatives of Differentiation-Inducing Factor 1 Differentially Control Chemotaxis and Stalk Cell Differentiation in Dictyostelium discoideum |
title_fullStr | Derivatives of Differentiation-Inducing Factor 1 Differentially Control Chemotaxis and Stalk Cell Differentiation in Dictyostelium discoideum |
title_full_unstemmed | Derivatives of Differentiation-Inducing Factor 1 Differentially Control Chemotaxis and Stalk Cell Differentiation in Dictyostelium discoideum |
title_short | Derivatives of Differentiation-Inducing Factor 1 Differentially Control Chemotaxis and Stalk Cell Differentiation in Dictyostelium discoideum |
title_sort | derivatives of differentiation-inducing factor 1 differentially control chemotaxis and stalk cell differentiation in dictyostelium discoideum |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10295651/ https://www.ncbi.nlm.nih.gov/pubmed/37372157 http://dx.doi.org/10.3390/biology12060873 |
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