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MicroRNA-142a-3p regulates neurogenic skeletal muscle atrophy by targeting Mef2a

Peripheral nerve injury can lead to progressive muscle atrophy and poor motor function recovery, which is a difficult point of treatment, and the mechanism needs to be further explored. In previous studies, we found that miR-142a-3p was significantly upregulated and persistently highly expressed in...

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Detalles Bibliográficos
Autores principales: Gu, Xinyi, Wang, Shen, Li, Dongdong, Jin, Bo, Qi, Zhidan, Deng, Jin, Huang, Chen, Yin, Xiaofeng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society of Gene & Cell Therapy 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10362021/
https://www.ncbi.nlm.nih.gov/pubmed/37483274
http://dx.doi.org/10.1016/j.omtn.2023.05.023
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author Gu, Xinyi
Wang, Shen
Li, Dongdong
Jin, Bo
Qi, Zhidan
Deng, Jin
Huang, Chen
Yin, Xiaofeng
author_facet Gu, Xinyi
Wang, Shen
Li, Dongdong
Jin, Bo
Qi, Zhidan
Deng, Jin
Huang, Chen
Yin, Xiaofeng
author_sort Gu, Xinyi
collection PubMed
description Peripheral nerve injury can lead to progressive muscle atrophy and poor motor function recovery, which is a difficult point of treatment, and the mechanism needs to be further explored. In previous studies, we found that miR-142a-3p was significantly upregulated and persistently highly expressed in denervated mouse skeletal muscle. Here, we show that overexpression of miR-142a-3p inhibited the growth and differentiation of C2C12 myoblast, while knockdown of miR-142a-3p had a promoting effect. In vitro, knockdown of miR-142a-3p in denervated mouse skeletal muscle effectively increased proliferating muscle satellite cells and ameliorated muscle atrophy. Mechanistically, the myoregulator Mef2a was proved to be an important downstream target of miR-142a-3p, and miR-142a-3p regulates skeletal muscle differentiation and regeneration by inhibiting the expression of Mef2a. The co-knockdown of Mef2a and miR-142a-3p effectively alleviated or offset the biological effects of miR-142a-3p knockdown. In conclusion, our data revealed that miR-142a-3p regulates neurogenic skeletal muscle atrophy by targeting Mef2a.
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spelling pubmed-103620212023-07-23 MicroRNA-142a-3p regulates neurogenic skeletal muscle atrophy by targeting Mef2a Gu, Xinyi Wang, Shen Li, Dongdong Jin, Bo Qi, Zhidan Deng, Jin Huang, Chen Yin, Xiaofeng Mol Ther Nucleic Acids Original Article Peripheral nerve injury can lead to progressive muscle atrophy and poor motor function recovery, which is a difficult point of treatment, and the mechanism needs to be further explored. In previous studies, we found that miR-142a-3p was significantly upregulated and persistently highly expressed in denervated mouse skeletal muscle. Here, we show that overexpression of miR-142a-3p inhibited the growth and differentiation of C2C12 myoblast, while knockdown of miR-142a-3p had a promoting effect. In vitro, knockdown of miR-142a-3p in denervated mouse skeletal muscle effectively increased proliferating muscle satellite cells and ameliorated muscle atrophy. Mechanistically, the myoregulator Mef2a was proved to be an important downstream target of miR-142a-3p, and miR-142a-3p regulates skeletal muscle differentiation and regeneration by inhibiting the expression of Mef2a. The co-knockdown of Mef2a and miR-142a-3p effectively alleviated or offset the biological effects of miR-142a-3p knockdown. In conclusion, our data revealed that miR-142a-3p regulates neurogenic skeletal muscle atrophy by targeting Mef2a. American Society of Gene & Cell Therapy 2023-06-03 /pmc/articles/PMC10362021/ /pubmed/37483274 http://dx.doi.org/10.1016/j.omtn.2023.05.023 Text en © 2023 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Original Article
Gu, Xinyi
Wang, Shen
Li, Dongdong
Jin, Bo
Qi, Zhidan
Deng, Jin
Huang, Chen
Yin, Xiaofeng
MicroRNA-142a-3p regulates neurogenic skeletal muscle atrophy by targeting Mef2a
title MicroRNA-142a-3p regulates neurogenic skeletal muscle atrophy by targeting Mef2a
title_full MicroRNA-142a-3p regulates neurogenic skeletal muscle atrophy by targeting Mef2a
title_fullStr MicroRNA-142a-3p regulates neurogenic skeletal muscle atrophy by targeting Mef2a
title_full_unstemmed MicroRNA-142a-3p regulates neurogenic skeletal muscle atrophy by targeting Mef2a
title_short MicroRNA-142a-3p regulates neurogenic skeletal muscle atrophy by targeting Mef2a
title_sort microrna-142a-3p regulates neurogenic skeletal muscle atrophy by targeting mef2a
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10362021/
https://www.ncbi.nlm.nih.gov/pubmed/37483274
http://dx.doi.org/10.1016/j.omtn.2023.05.023
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