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MicroRNA-142a-3p regulates neurogenic skeletal muscle atrophy by targeting Mef2a
Peripheral nerve injury can lead to progressive muscle atrophy and poor motor function recovery, which is a difficult point of treatment, and the mechanism needs to be further explored. In previous studies, we found that miR-142a-3p was significantly upregulated and persistently highly expressed in...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Society of Gene & Cell Therapy
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10362021/ https://www.ncbi.nlm.nih.gov/pubmed/37483274 http://dx.doi.org/10.1016/j.omtn.2023.05.023 |
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author | Gu, Xinyi Wang, Shen Li, Dongdong Jin, Bo Qi, Zhidan Deng, Jin Huang, Chen Yin, Xiaofeng |
author_facet | Gu, Xinyi Wang, Shen Li, Dongdong Jin, Bo Qi, Zhidan Deng, Jin Huang, Chen Yin, Xiaofeng |
author_sort | Gu, Xinyi |
collection | PubMed |
description | Peripheral nerve injury can lead to progressive muscle atrophy and poor motor function recovery, which is a difficult point of treatment, and the mechanism needs to be further explored. In previous studies, we found that miR-142a-3p was significantly upregulated and persistently highly expressed in denervated mouse skeletal muscle. Here, we show that overexpression of miR-142a-3p inhibited the growth and differentiation of C2C12 myoblast, while knockdown of miR-142a-3p had a promoting effect. In vitro, knockdown of miR-142a-3p in denervated mouse skeletal muscle effectively increased proliferating muscle satellite cells and ameliorated muscle atrophy. Mechanistically, the myoregulator Mef2a was proved to be an important downstream target of miR-142a-3p, and miR-142a-3p regulates skeletal muscle differentiation and regeneration by inhibiting the expression of Mef2a. The co-knockdown of Mef2a and miR-142a-3p effectively alleviated or offset the biological effects of miR-142a-3p knockdown. In conclusion, our data revealed that miR-142a-3p regulates neurogenic skeletal muscle atrophy by targeting Mef2a. |
format | Online Article Text |
id | pubmed-10362021 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | American Society of Gene & Cell Therapy |
record_format | MEDLINE/PubMed |
spelling | pubmed-103620212023-07-23 MicroRNA-142a-3p regulates neurogenic skeletal muscle atrophy by targeting Mef2a Gu, Xinyi Wang, Shen Li, Dongdong Jin, Bo Qi, Zhidan Deng, Jin Huang, Chen Yin, Xiaofeng Mol Ther Nucleic Acids Original Article Peripheral nerve injury can lead to progressive muscle atrophy and poor motor function recovery, which is a difficult point of treatment, and the mechanism needs to be further explored. In previous studies, we found that miR-142a-3p was significantly upregulated and persistently highly expressed in denervated mouse skeletal muscle. Here, we show that overexpression of miR-142a-3p inhibited the growth and differentiation of C2C12 myoblast, while knockdown of miR-142a-3p had a promoting effect. In vitro, knockdown of miR-142a-3p in denervated mouse skeletal muscle effectively increased proliferating muscle satellite cells and ameliorated muscle atrophy. Mechanistically, the myoregulator Mef2a was proved to be an important downstream target of miR-142a-3p, and miR-142a-3p regulates skeletal muscle differentiation and regeneration by inhibiting the expression of Mef2a. The co-knockdown of Mef2a and miR-142a-3p effectively alleviated or offset the biological effects of miR-142a-3p knockdown. In conclusion, our data revealed that miR-142a-3p regulates neurogenic skeletal muscle atrophy by targeting Mef2a. American Society of Gene & Cell Therapy 2023-06-03 /pmc/articles/PMC10362021/ /pubmed/37483274 http://dx.doi.org/10.1016/j.omtn.2023.05.023 Text en © 2023 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Original Article Gu, Xinyi Wang, Shen Li, Dongdong Jin, Bo Qi, Zhidan Deng, Jin Huang, Chen Yin, Xiaofeng MicroRNA-142a-3p regulates neurogenic skeletal muscle atrophy by targeting Mef2a |
title | MicroRNA-142a-3p regulates neurogenic skeletal muscle atrophy by targeting Mef2a |
title_full | MicroRNA-142a-3p regulates neurogenic skeletal muscle atrophy by targeting Mef2a |
title_fullStr | MicroRNA-142a-3p regulates neurogenic skeletal muscle atrophy by targeting Mef2a |
title_full_unstemmed | MicroRNA-142a-3p regulates neurogenic skeletal muscle atrophy by targeting Mef2a |
title_short | MicroRNA-142a-3p regulates neurogenic skeletal muscle atrophy by targeting Mef2a |
title_sort | microrna-142a-3p regulates neurogenic skeletal muscle atrophy by targeting mef2a |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10362021/ https://www.ncbi.nlm.nih.gov/pubmed/37483274 http://dx.doi.org/10.1016/j.omtn.2023.05.023 |
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