CircECE1 promotes osteosarcoma progression through regulating RAB3D by sponging miR-588
BACKGROUND: Circular RNAs (circRNAs) have been confirmed to be involved in cancer pathogenesis. However, the underlying mechanism of circRNA endothelin converting enzyme 1 (circECE1) in osteosarcoma (OS) development is still not understood. METHODS: The expression levels of circECE1, microRNA-588 (m...
Autores principales: | , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10410784/ https://www.ncbi.nlm.nih.gov/pubmed/37559140 http://dx.doi.org/10.1186/s13018-023-04045-4 |
_version_ | 1785086525132767232 |
---|---|
author | Liang, Zhizhong Shi, Yuxia Guan, Zhe |
author_facet | Liang, Zhizhong Shi, Yuxia Guan, Zhe |
author_sort | Liang, Zhizhong |
collection | PubMed |
description | BACKGROUND: Circular RNAs (circRNAs) have been confirmed to be involved in cancer pathogenesis. However, the underlying mechanism of circRNA endothelin converting enzyme 1 (circECE1) in osteosarcoma (OS) development is still not understood. METHODS: The expression levels of circECE1, microRNA-588 (miR-588) and RAB3D, member RAS oncogene family (RAB3D) were gauged by quantitative real-time polymerase chain reaction (qRT-PCR) and western blot. OS cell proliferation was assessed by cell counting kit-8 (CCK-8) assay and 5-ethynyl-2’-deoxyuridine (EdU) assay. OS cell apoptosis rate and metastasis were identified by flow cytometry and transwell assay. Dual-luciferase reporter analysis and RNA immunoprecipitation (RIP) assay were performed to confirm the interactions among circECE1, miR-588 and RAB3D. Xenograft tumor models were established to explore circECE1 function in vivo. Immunohistochemistry (IHC) assay was applied to analyze RAB3D level after circECE1 knockdown. RESULTS: In OS, circECE1 expression was higher than that in normal chondroma tissues. High levels of circECE1 were positively linked to OS cell viability, proliferation, migration and invasion, and negatively linked to OS cell apoptosis rate. It was found that circECE1 was a miR-588 sponge, and miR-588 inhibitor abrogated the influence of si-circECE1 on OS cells. MiR-588 targeted RAB3D to further regulate the pathological process of OS. Moreover, silencing circECE1 blocked OS tumor growth in vivo. CONCLUSION: We elucidated the function of a novel circECE1/miR-588/RAB3D axis in OS progression. |
format | Online Article Text |
id | pubmed-10410784 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-104107842023-08-10 CircECE1 promotes osteosarcoma progression through regulating RAB3D by sponging miR-588 Liang, Zhizhong Shi, Yuxia Guan, Zhe J Orthop Surg Res Research Article BACKGROUND: Circular RNAs (circRNAs) have been confirmed to be involved in cancer pathogenesis. However, the underlying mechanism of circRNA endothelin converting enzyme 1 (circECE1) in osteosarcoma (OS) development is still not understood. METHODS: The expression levels of circECE1, microRNA-588 (miR-588) and RAB3D, member RAS oncogene family (RAB3D) were gauged by quantitative real-time polymerase chain reaction (qRT-PCR) and western blot. OS cell proliferation was assessed by cell counting kit-8 (CCK-8) assay and 5-ethynyl-2’-deoxyuridine (EdU) assay. OS cell apoptosis rate and metastasis were identified by flow cytometry and transwell assay. Dual-luciferase reporter analysis and RNA immunoprecipitation (RIP) assay were performed to confirm the interactions among circECE1, miR-588 and RAB3D. Xenograft tumor models were established to explore circECE1 function in vivo. Immunohistochemistry (IHC) assay was applied to analyze RAB3D level after circECE1 knockdown. RESULTS: In OS, circECE1 expression was higher than that in normal chondroma tissues. High levels of circECE1 were positively linked to OS cell viability, proliferation, migration and invasion, and negatively linked to OS cell apoptosis rate. It was found that circECE1 was a miR-588 sponge, and miR-588 inhibitor abrogated the influence of si-circECE1 on OS cells. MiR-588 targeted RAB3D to further regulate the pathological process of OS. Moreover, silencing circECE1 blocked OS tumor growth in vivo. CONCLUSION: We elucidated the function of a novel circECE1/miR-588/RAB3D axis in OS progression. BioMed Central 2023-08-09 /pmc/articles/PMC10410784/ /pubmed/37559140 http://dx.doi.org/10.1186/s13018-023-04045-4 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Article Liang, Zhizhong Shi, Yuxia Guan, Zhe CircECE1 promotes osteosarcoma progression through regulating RAB3D by sponging miR-588 |
title | CircECE1 promotes osteosarcoma progression through regulating RAB3D by sponging miR-588 |
title_full | CircECE1 promotes osteosarcoma progression through regulating RAB3D by sponging miR-588 |
title_fullStr | CircECE1 promotes osteosarcoma progression through regulating RAB3D by sponging miR-588 |
title_full_unstemmed | CircECE1 promotes osteosarcoma progression through regulating RAB3D by sponging miR-588 |
title_short | CircECE1 promotes osteosarcoma progression through regulating RAB3D by sponging miR-588 |
title_sort | circece1 promotes osteosarcoma progression through regulating rab3d by sponging mir-588 |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10410784/ https://www.ncbi.nlm.nih.gov/pubmed/37559140 http://dx.doi.org/10.1186/s13018-023-04045-4 |
work_keys_str_mv | AT liangzhizhong circece1promotesosteosarcomaprogressionthroughregulatingrab3dbyspongingmir588 AT shiyuxia circece1promotesosteosarcomaprogressionthroughregulatingrab3dbyspongingmir588 AT guanzhe circece1promotesosteosarcomaprogressionthroughregulatingrab3dbyspongingmir588 |