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In vivo acoustic patterning of endothelial cells for tissue vascularization

Strategies to fabricate microvascular networks that structurally and functionally mimic native microvessels are needed to address a host of clinical conditions associated with tissue ischemia. The objective of this work was to advance a novel ultrasound technology to fabricate complex, functional mi...

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Autores principales: Comeau, Eric S., Vander Horst, Melinda A., Raeman, Carol H., Child, Sally Z., Hocking, Denise C., Dalecki, Diane
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10522665/
https://www.ncbi.nlm.nih.gov/pubmed/37752255
http://dx.doi.org/10.1038/s41598-023-43299-0
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author Comeau, Eric S.
Vander Horst, Melinda A.
Raeman, Carol H.
Child, Sally Z.
Hocking, Denise C.
Dalecki, Diane
author_facet Comeau, Eric S.
Vander Horst, Melinda A.
Raeman, Carol H.
Child, Sally Z.
Hocking, Denise C.
Dalecki, Diane
author_sort Comeau, Eric S.
collection PubMed
description Strategies to fabricate microvascular networks that structurally and functionally mimic native microvessels are needed to address a host of clinical conditions associated with tissue ischemia. The objective of this work was to advance a novel ultrasound technology to fabricate complex, functional microvascular networks directly in vivo. Acoustic patterning utilizes forces within an ultrasound standing wave field (USWF) to organize cells or microparticles volumetrically into defined geometric assemblies. A dual-transducer system was developed to generate USWFs site-specifically in vivo through interference of two ultrasound fields. The system rapidly patterned injected cells or microparticles into parallel sheets within collagen hydrogels in vivo. Acoustic patterning of injected endothelial cells within flanks of immunodeficient mice gave rise to perfused microvessels within 7 days of patterning, whereas non-patterned cells did not survive. Thus, externally-applied ultrasound fields guided injected endothelial cells to self-assemble into perfused microvascular networks in vivo. These studies advance acoustic patterning towards in vivo tissue engineering by providing the first proof-of-concept demonstration that non-invasive, ultrasound-mediated cell patterning can be used to fabricate functional microvascular networks directly in vivo.
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spelling pubmed-105226652023-09-28 In vivo acoustic patterning of endothelial cells for tissue vascularization Comeau, Eric S. Vander Horst, Melinda A. Raeman, Carol H. Child, Sally Z. Hocking, Denise C. Dalecki, Diane Sci Rep Article Strategies to fabricate microvascular networks that structurally and functionally mimic native microvessels are needed to address a host of clinical conditions associated with tissue ischemia. The objective of this work was to advance a novel ultrasound technology to fabricate complex, functional microvascular networks directly in vivo. Acoustic patterning utilizes forces within an ultrasound standing wave field (USWF) to organize cells or microparticles volumetrically into defined geometric assemblies. A dual-transducer system was developed to generate USWFs site-specifically in vivo through interference of two ultrasound fields. The system rapidly patterned injected cells or microparticles into parallel sheets within collagen hydrogels in vivo. Acoustic patterning of injected endothelial cells within flanks of immunodeficient mice gave rise to perfused microvessels within 7 days of patterning, whereas non-patterned cells did not survive. Thus, externally-applied ultrasound fields guided injected endothelial cells to self-assemble into perfused microvascular networks in vivo. These studies advance acoustic patterning towards in vivo tissue engineering by providing the first proof-of-concept demonstration that non-invasive, ultrasound-mediated cell patterning can be used to fabricate functional microvascular networks directly in vivo. Nature Publishing Group UK 2023-09-26 /pmc/articles/PMC10522665/ /pubmed/37752255 http://dx.doi.org/10.1038/s41598-023-43299-0 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Comeau, Eric S.
Vander Horst, Melinda A.
Raeman, Carol H.
Child, Sally Z.
Hocking, Denise C.
Dalecki, Diane
In vivo acoustic patterning of endothelial cells for tissue vascularization
title In vivo acoustic patterning of endothelial cells for tissue vascularization
title_full In vivo acoustic patterning of endothelial cells for tissue vascularization
title_fullStr In vivo acoustic patterning of endothelial cells for tissue vascularization
title_full_unstemmed In vivo acoustic patterning of endothelial cells for tissue vascularization
title_short In vivo acoustic patterning of endothelial cells for tissue vascularization
title_sort in vivo acoustic patterning of endothelial cells for tissue vascularization
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10522665/
https://www.ncbi.nlm.nih.gov/pubmed/37752255
http://dx.doi.org/10.1038/s41598-023-43299-0
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