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Identifying the most suitable endogenous control for determining gene expression in hearts from organ donors

BACKGROUND: Quantitative real-time reverse transcription PCR (qRT-PCR) is a useful tool for assessing gene expression in different tissues, but the choice of adequate controls is critical to normalise the results, thereby avoiding differences and maximizing sensitivity and accuracy. So far, many gen...

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Detalles Bibliográficos
Autores principales: Pérez, Silvia, Royo, Luis J, Astudillo, Aurora, Escudero, Dolores, Álvarez, Francisco, Rodríguez, Aida, Gómez, Enrique, Otero, Jesús
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2007
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2234425/
https://www.ncbi.nlm.nih.gov/pubmed/18096027
http://dx.doi.org/10.1186/1471-2199-8-114