Insight in modulation of inflammation in response to diclofenac intervention: a human intervention study

BACKGROUND: Chronic systemic low-grade inflammation in obese subjects is associated with health complications including cardiovascular diseases, insulin resistance and diabetes. Reducing inflammatory responses may reduce these risks. However, available markers of inflammatory status inadequately des...

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Autores principales: van Erk, Marjan J, Wopereis, Suzan, Rubingh, Carina, van Vliet, Trinette, Verheij, Elwin, Cnubben, Nicole HP, Pedersen, Theresa L, Newman, John W, Smilde, Age K, Greef, Jan van der, Hendriks, Henk FJ, van Ommen, Ben
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2837611/
https://www.ncbi.nlm.nih.gov/pubmed/20178593
http://dx.doi.org/10.1186/1755-8794-3-5
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author van Erk, Marjan J
Wopereis, Suzan
Rubingh, Carina
van Vliet, Trinette
Verheij, Elwin
Cnubben, Nicole HP
Pedersen, Theresa L
Newman, John W
Smilde, Age K
Greef, Jan van der
Hendriks, Henk FJ
van Ommen, Ben
author_facet van Erk, Marjan J
Wopereis, Suzan
Rubingh, Carina
van Vliet, Trinette
Verheij, Elwin
Cnubben, Nicole HP
Pedersen, Theresa L
Newman, John W
Smilde, Age K
Greef, Jan van der
Hendriks, Henk FJ
van Ommen, Ben
author_sort van Erk, Marjan J
collection PubMed
description BACKGROUND: Chronic systemic low-grade inflammation in obese subjects is associated with health complications including cardiovascular diseases, insulin resistance and diabetes. Reducing inflammatory responses may reduce these risks. However, available markers of inflammatory status inadequately describe the complexity of metabolic responses to mild anti-inflammatory therapy. METHODS: To address this limitation, we used an integrative omics approach to characterize modulation of inflammation in overweight men during an intervention with the non-steroidal anti-inflammatory drug diclofenac. Measured parameters included 80 plasma proteins, >300 plasma metabolites (lipids, free fatty acids, oxylipids and polar compounds) and an array of peripheral blood mononuclear cells (PBMC) gene expression products. These measures were submitted to multivariate and correlation analysis and were used for construction of biological response networks. RESULTS: A panel of genes, proteins and metabolites, including PGE(2 )and TNF-alpha, were identified that describe a diclofenac-response network (68 genes in PBMC, 1 plasma protein and 4 plasma metabolites). Novel candidate markers of inflammatory modulation included PBMC expression of annexin A1 and caspase 8, and the arachidonic acid metabolite 5,6-DHET. CONCLUSION: In this study the integrated analysis of a wide range of parameters allowed the development of a network of markers responding to inflammatory modulation, thereby providing insight into the complex process of inflammation and ways to assess changes in inflammatory status associated with obesity. TRIAL REGISTRATION: The study is registered as NCT00221052 in clinicaltrials.gov database.
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spelling pubmed-28376112010-03-13 Insight in modulation of inflammation in response to diclofenac intervention: a human intervention study van Erk, Marjan J Wopereis, Suzan Rubingh, Carina van Vliet, Trinette Verheij, Elwin Cnubben, Nicole HP Pedersen, Theresa L Newman, John W Smilde, Age K Greef, Jan van der Hendriks, Henk FJ van Ommen, Ben BMC Med Genomics Research article BACKGROUND: Chronic systemic low-grade inflammation in obese subjects is associated with health complications including cardiovascular diseases, insulin resistance and diabetes. Reducing inflammatory responses may reduce these risks. However, available markers of inflammatory status inadequately describe the complexity of metabolic responses to mild anti-inflammatory therapy. METHODS: To address this limitation, we used an integrative omics approach to characterize modulation of inflammation in overweight men during an intervention with the non-steroidal anti-inflammatory drug diclofenac. Measured parameters included 80 plasma proteins, >300 plasma metabolites (lipids, free fatty acids, oxylipids and polar compounds) and an array of peripheral blood mononuclear cells (PBMC) gene expression products. These measures were submitted to multivariate and correlation analysis and were used for construction of biological response networks. RESULTS: A panel of genes, proteins and metabolites, including PGE(2 )and TNF-alpha, were identified that describe a diclofenac-response network (68 genes in PBMC, 1 plasma protein and 4 plasma metabolites). Novel candidate markers of inflammatory modulation included PBMC expression of annexin A1 and caspase 8, and the arachidonic acid metabolite 5,6-DHET. CONCLUSION: In this study the integrated analysis of a wide range of parameters allowed the development of a network of markers responding to inflammatory modulation, thereby providing insight into the complex process of inflammation and ways to assess changes in inflammatory status associated with obesity. TRIAL REGISTRATION: The study is registered as NCT00221052 in clinicaltrials.gov database. BioMed Central 2010-02-23 /pmc/articles/PMC2837611/ /pubmed/20178593 http://dx.doi.org/10.1186/1755-8794-3-5 Text en Copyright ©2010 van Erk et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research article
van Erk, Marjan J
Wopereis, Suzan
Rubingh, Carina
van Vliet, Trinette
Verheij, Elwin
Cnubben, Nicole HP
Pedersen, Theresa L
Newman, John W
Smilde, Age K
Greef, Jan van der
Hendriks, Henk FJ
van Ommen, Ben
Insight in modulation of inflammation in response to diclofenac intervention: a human intervention study
title Insight in modulation of inflammation in response to diclofenac intervention: a human intervention study
title_full Insight in modulation of inflammation in response to diclofenac intervention: a human intervention study
title_fullStr Insight in modulation of inflammation in response to diclofenac intervention: a human intervention study
title_full_unstemmed Insight in modulation of inflammation in response to diclofenac intervention: a human intervention study
title_short Insight in modulation of inflammation in response to diclofenac intervention: a human intervention study
title_sort insight in modulation of inflammation in response to diclofenac intervention: a human intervention study
topic Research article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2837611/
https://www.ncbi.nlm.nih.gov/pubmed/20178593
http://dx.doi.org/10.1186/1755-8794-3-5
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