Obesity induced by a pair-fed high fat sucrose diet: methylation and expression pattern of genes related to energy homeostasis

BACKGROUND: The expression of some genes controlling energy homeostasis could be regulated by epigenetic mechanisms that may play a role in body weight regulation. Thus, it is known that various nutritional factors affect DNA methylation. In order to assess whether the macronutrient composition of t...

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Autores principales: Lomba, Almudena, Milagro, Fermín I, García-Díaz, Diego F, Marti, Amelia, Campión, Javier, Martínez, J Alfredo
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2909242/
https://www.ncbi.nlm.nih.gov/pubmed/20534152
http://dx.doi.org/10.1186/1476-511X-9-60
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author Lomba, Almudena
Milagro, Fermín I
García-Díaz, Diego F
Marti, Amelia
Campión, Javier
Martínez, J Alfredo
author_facet Lomba, Almudena
Milagro, Fermín I
García-Díaz, Diego F
Marti, Amelia
Campión, Javier
Martínez, J Alfredo
author_sort Lomba, Almudena
collection PubMed
description BACKGROUND: The expression of some genes controlling energy homeostasis could be regulated by epigenetic mechanisms that may play a role in body weight regulation. Thus, it is known that various nutritional factors affect DNA methylation. In order to assess whether the macronutrient composition of the diet could be related to the epigenetic regulation of gene expression and with obesity development, we investigated the effects on methylation and expression patterns of two pair-fed isocaloric diets in rats: control (rich in starch) and HFS (rich in fat and sucrose). RESULTS: The pair-fed HFS diet induced higher weight gain and adiposity as compared to the controls as well as liver triglyceride accumulation and oxidative stress. Feeding the HFS diet impaired glucose tolerance and serum triglycerides and cholesterol. Liver glucokinase expression, a key glycolytic gene, remained unaltered, as well as the mRNA values of fatty acid synthase and NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 6 (NDUFB6) in liver and visceral adipocytes, which regulate lipogenesis and mitochondrial oxidative metabolism, respectively. Liver expression of hydroxyacyl-coenzyme A dehydrogenase (HADHB), a key gene of β-oxidation pathway, was higher in the HFS-fed animals. However, the methylation status of CpG islands in HADHB and glucokinase genes remained unchanged after feeding the HFS diet. CONCLUSIONS: These results confirm that the distribution and type of macronutrients (starch vs. sucrose, and percent of fat) influence obesity onset and the associated metabolic complications. HFS diets produce obesity independently of total energy intake, although apparently no epigenetic (DNA methylation) changes accompanied the modifications observed in gene expression.
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spelling pubmed-29092422010-07-24 Obesity induced by a pair-fed high fat sucrose diet: methylation and expression pattern of genes related to energy homeostasis Lomba, Almudena Milagro, Fermín I García-Díaz, Diego F Marti, Amelia Campión, Javier Martínez, J Alfredo Lipids Health Dis Research BACKGROUND: The expression of some genes controlling energy homeostasis could be regulated by epigenetic mechanisms that may play a role in body weight regulation. Thus, it is known that various nutritional factors affect DNA methylation. In order to assess whether the macronutrient composition of the diet could be related to the epigenetic regulation of gene expression and with obesity development, we investigated the effects on methylation and expression patterns of two pair-fed isocaloric diets in rats: control (rich in starch) and HFS (rich in fat and sucrose). RESULTS: The pair-fed HFS diet induced higher weight gain and adiposity as compared to the controls as well as liver triglyceride accumulation and oxidative stress. Feeding the HFS diet impaired glucose tolerance and serum triglycerides and cholesterol. Liver glucokinase expression, a key glycolytic gene, remained unaltered, as well as the mRNA values of fatty acid synthase and NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 6 (NDUFB6) in liver and visceral adipocytes, which regulate lipogenesis and mitochondrial oxidative metabolism, respectively. Liver expression of hydroxyacyl-coenzyme A dehydrogenase (HADHB), a key gene of β-oxidation pathway, was higher in the HFS-fed animals. However, the methylation status of CpG islands in HADHB and glucokinase genes remained unchanged after feeding the HFS diet. CONCLUSIONS: These results confirm that the distribution and type of macronutrients (starch vs. sucrose, and percent of fat) influence obesity onset and the associated metabolic complications. HFS diets produce obesity independently of total energy intake, although apparently no epigenetic (DNA methylation) changes accompanied the modifications observed in gene expression. BioMed Central 2010-06-09 /pmc/articles/PMC2909242/ /pubmed/20534152 http://dx.doi.org/10.1186/1476-511X-9-60 Text en Copyright ©2010 Lomba et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Lomba, Almudena
Milagro, Fermín I
García-Díaz, Diego F
Marti, Amelia
Campión, Javier
Martínez, J Alfredo
Obesity induced by a pair-fed high fat sucrose diet: methylation and expression pattern of genes related to energy homeostasis
title Obesity induced by a pair-fed high fat sucrose diet: methylation and expression pattern of genes related to energy homeostasis
title_full Obesity induced by a pair-fed high fat sucrose diet: methylation and expression pattern of genes related to energy homeostasis
title_fullStr Obesity induced by a pair-fed high fat sucrose diet: methylation and expression pattern of genes related to energy homeostasis
title_full_unstemmed Obesity induced by a pair-fed high fat sucrose diet: methylation and expression pattern of genes related to energy homeostasis
title_short Obesity induced by a pair-fed high fat sucrose diet: methylation and expression pattern of genes related to energy homeostasis
title_sort obesity induced by a pair-fed high fat sucrose diet: methylation and expression pattern of genes related to energy homeostasis
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2909242/
https://www.ncbi.nlm.nih.gov/pubmed/20534152
http://dx.doi.org/10.1186/1476-511X-9-60
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