Isoflurane's Effect on Protein Conformation as a Proposed Mechanism for Preconditioning

Persistent alteration of protein conformation due to interaction with isoflurane may be a novel molecular aspect of preconditioning. We preincubated human serum albumin with isoflurane, dialyzed to release agent, and assessed protein conformation. Susceptibility to chemical modification by methylgly...

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Autores principales: Baker, Michelle R., Benton, Sean K., Theisen, Christopher S., McClintick, Chad A., Fibuch, Eugene E., Seidler, Norbert W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3171779/
https://www.ncbi.nlm.nih.gov/pubmed/21918721
http://dx.doi.org/10.1155/2011/739712
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author Baker, Michelle R.
Benton, Sean K.
Theisen, Christopher S.
McClintick, Chad A.
Fibuch, Eugene E.
Seidler, Norbert W.
author_facet Baker, Michelle R.
Benton, Sean K.
Theisen, Christopher S.
McClintick, Chad A.
Fibuch, Eugene E.
Seidler, Norbert W.
author_sort Baker, Michelle R.
collection PubMed
description Persistent alteration of protein conformation due to interaction with isoflurane may be a novel molecular aspect of preconditioning. We preincubated human serum albumin with isoflurane, dialyzed to release agent, and assessed protein conformation. Susceptibility to chemical modification by methylglyoxal and nitrophenylacetate was also examined. Isoflurane had a persistent effect on protein conformation. An increase in the susceptibility of surface residues to chemical modification attended this change in conformation. Modification of isoflurane-treated HSA included intra- and intersubunit cross-linking that may be a consequence of anesthetic-induced changes in multimeric subpopulations. This irreversible effect of isoflurane may represent a mechanism for preconditioning.
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spelling pubmed-31717792011-09-14 Isoflurane's Effect on Protein Conformation as a Proposed Mechanism for Preconditioning Baker, Michelle R. Benton, Sean K. Theisen, Christopher S. McClintick, Chad A. Fibuch, Eugene E. Seidler, Norbert W. Biochem Res Int Research Article Persistent alteration of protein conformation due to interaction with isoflurane may be a novel molecular aspect of preconditioning. We preincubated human serum albumin with isoflurane, dialyzed to release agent, and assessed protein conformation. Susceptibility to chemical modification by methylglyoxal and nitrophenylacetate was also examined. Isoflurane had a persistent effect on protein conformation. An increase in the susceptibility of surface residues to chemical modification attended this change in conformation. Modification of isoflurane-treated HSA included intra- and intersubunit cross-linking that may be a consequence of anesthetic-induced changes in multimeric subpopulations. This irreversible effect of isoflurane may represent a mechanism for preconditioning. Hindawi Publishing Corporation 2011 2011-09-12 /pmc/articles/PMC3171779/ /pubmed/21918721 http://dx.doi.org/10.1155/2011/739712 Text en Copyright © 2011 Michelle R. Baker et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Baker, Michelle R.
Benton, Sean K.
Theisen, Christopher S.
McClintick, Chad A.
Fibuch, Eugene E.
Seidler, Norbert W.
Isoflurane's Effect on Protein Conformation as a Proposed Mechanism for Preconditioning
title Isoflurane's Effect on Protein Conformation as a Proposed Mechanism for Preconditioning
title_full Isoflurane's Effect on Protein Conformation as a Proposed Mechanism for Preconditioning
title_fullStr Isoflurane's Effect on Protein Conformation as a Proposed Mechanism for Preconditioning
title_full_unstemmed Isoflurane's Effect on Protein Conformation as a Proposed Mechanism for Preconditioning
title_short Isoflurane's Effect on Protein Conformation as a Proposed Mechanism for Preconditioning
title_sort isoflurane's effect on protein conformation as a proposed mechanism for preconditioning
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3171779/
https://www.ncbi.nlm.nih.gov/pubmed/21918721
http://dx.doi.org/10.1155/2011/739712
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