Isoflurane's Effect on Protein Conformation as a Proposed Mechanism for Preconditioning
Persistent alteration of protein conformation due to interaction with isoflurane may be a novel molecular aspect of preconditioning. We preincubated human serum albumin with isoflurane, dialyzed to release agent, and assessed protein conformation. Susceptibility to chemical modification by methylgly...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3171779/ https://www.ncbi.nlm.nih.gov/pubmed/21918721 http://dx.doi.org/10.1155/2011/739712 |
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author | Baker, Michelle R. Benton, Sean K. Theisen, Christopher S. McClintick, Chad A. Fibuch, Eugene E. Seidler, Norbert W. |
author_facet | Baker, Michelle R. Benton, Sean K. Theisen, Christopher S. McClintick, Chad A. Fibuch, Eugene E. Seidler, Norbert W. |
author_sort | Baker, Michelle R. |
collection | PubMed |
description | Persistent alteration of protein conformation due to interaction with isoflurane may be a novel molecular aspect of preconditioning. We preincubated human serum albumin with isoflurane, dialyzed to release agent, and assessed protein conformation. Susceptibility to chemical modification by methylglyoxal and nitrophenylacetate was also examined. Isoflurane had a persistent effect on protein conformation. An increase in the susceptibility of surface residues to chemical modification attended this change in conformation. Modification of isoflurane-treated HSA included intra- and intersubunit cross-linking that may be a consequence of anesthetic-induced changes in multimeric subpopulations. This irreversible effect of isoflurane may represent a mechanism for preconditioning. |
format | Online Article Text |
id | pubmed-3171779 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-31717792011-09-14 Isoflurane's Effect on Protein Conformation as a Proposed Mechanism for Preconditioning Baker, Michelle R. Benton, Sean K. Theisen, Christopher S. McClintick, Chad A. Fibuch, Eugene E. Seidler, Norbert W. Biochem Res Int Research Article Persistent alteration of protein conformation due to interaction with isoflurane may be a novel molecular aspect of preconditioning. We preincubated human serum albumin with isoflurane, dialyzed to release agent, and assessed protein conformation. Susceptibility to chemical modification by methylglyoxal and nitrophenylacetate was also examined. Isoflurane had a persistent effect on protein conformation. An increase in the susceptibility of surface residues to chemical modification attended this change in conformation. Modification of isoflurane-treated HSA included intra- and intersubunit cross-linking that may be a consequence of anesthetic-induced changes in multimeric subpopulations. This irreversible effect of isoflurane may represent a mechanism for preconditioning. Hindawi Publishing Corporation 2011 2011-09-12 /pmc/articles/PMC3171779/ /pubmed/21918721 http://dx.doi.org/10.1155/2011/739712 Text en Copyright © 2011 Michelle R. Baker et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Baker, Michelle R. Benton, Sean K. Theisen, Christopher S. McClintick, Chad A. Fibuch, Eugene E. Seidler, Norbert W. Isoflurane's Effect on Protein Conformation as a Proposed Mechanism for Preconditioning |
title | Isoflurane's Effect on Protein Conformation as a Proposed Mechanism for Preconditioning |
title_full | Isoflurane's Effect on Protein Conformation as a Proposed Mechanism for Preconditioning |
title_fullStr | Isoflurane's Effect on Protein Conformation as a Proposed Mechanism for Preconditioning |
title_full_unstemmed | Isoflurane's Effect on Protein Conformation as a Proposed Mechanism for Preconditioning |
title_short | Isoflurane's Effect on Protein Conformation as a Proposed Mechanism for Preconditioning |
title_sort | isoflurane's effect on protein conformation as a proposed mechanism for preconditioning |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3171779/ https://www.ncbi.nlm.nih.gov/pubmed/21918721 http://dx.doi.org/10.1155/2011/739712 |
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