Side population rather than CD133(+ )cells distinguishes enriched tumorigenicity in hTERT-immortalized primary prostate cancer cells
BACKGROUND: Subpopulations of cancer cells with the capacity of generating solid tumors have been characterized. In various cancer types, including prostate cancer cells, a side population (SP) and CD133-expressing cells have been proposed as containing a population cancer cells with stem-like abili...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3180433/ https://www.ncbi.nlm.nih.gov/pubmed/21917149 http://dx.doi.org/10.1186/1476-4598-10-112 |
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author | Zhou, Jianjun Wang, Honghe Cannon, Virginetta Wolcott, Karen Marie Song, Hongbin Yates, Clayton |
author_facet | Zhou, Jianjun Wang, Honghe Cannon, Virginetta Wolcott, Karen Marie Song, Hongbin Yates, Clayton |
author_sort | Zhou, Jianjun |
collection | PubMed |
description | BACKGROUND: Subpopulations of cancer cells with the capacity of generating solid tumors have been characterized. In various cancer types, including prostate cancer cells, a side population (SP) and CD133-expressing cells have been proposed as containing a population cancer cells with stem-like ability. Therefore the aim of this work was to determine, in prostate cancer cell lines, the frequency and tumorigenic potential of SP and CD133+ cells. RESULTS: In vitro 2D colony-forming assay and sphere-forming assay, Flow cytometry analysis and magnetic cell sorting were utilized to sort CD133+, CD133- and Side population (SP) cells. Our findings indicate that CD44 and integrin α-6 are uniformly expressed in the hTERT cell lines; however, CD133 is expressed only in a small population (< 0.1%). FACS-sorted CD133+ and CD133- cells exhibited similar tumorigenicity in vitro and in vivo. Additionally, for the hTERT cells, SP rather than CD133 expression showed an 8-fold enhanced tumorigenic potential. The data suggest that SP cells, rather than those with CD133 marker, contain the rare population of CSC capable of producing prostate tumors. CONCLUSION: Collectively, our data suggest that although CD133 is expressed only in a small population of hTERT-immortalized prostate cancer cells, it is not likely to be associated with stem cells, as CD133- and CD133+ cells exhibited similar tumorigenicity. However, SP isolated cells, appear to be enriched with tumorigenic stem-like cells capable of generating palpable tumors. |
format | Online Article Text |
id | pubmed-3180433 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-31804332011-09-27 Side population rather than CD133(+ )cells distinguishes enriched tumorigenicity in hTERT-immortalized primary prostate cancer cells Zhou, Jianjun Wang, Honghe Cannon, Virginetta Wolcott, Karen Marie Song, Hongbin Yates, Clayton Mol Cancer Research BACKGROUND: Subpopulations of cancer cells with the capacity of generating solid tumors have been characterized. In various cancer types, including prostate cancer cells, a side population (SP) and CD133-expressing cells have been proposed as containing a population cancer cells with stem-like ability. Therefore the aim of this work was to determine, in prostate cancer cell lines, the frequency and tumorigenic potential of SP and CD133+ cells. RESULTS: In vitro 2D colony-forming assay and sphere-forming assay, Flow cytometry analysis and magnetic cell sorting were utilized to sort CD133+, CD133- and Side population (SP) cells. Our findings indicate that CD44 and integrin α-6 are uniformly expressed in the hTERT cell lines; however, CD133 is expressed only in a small population (< 0.1%). FACS-sorted CD133+ and CD133- cells exhibited similar tumorigenicity in vitro and in vivo. Additionally, for the hTERT cells, SP rather than CD133 expression showed an 8-fold enhanced tumorigenic potential. The data suggest that SP cells, rather than those with CD133 marker, contain the rare population of CSC capable of producing prostate tumors. CONCLUSION: Collectively, our data suggest that although CD133 is expressed only in a small population of hTERT-immortalized prostate cancer cells, it is not likely to be associated with stem cells, as CD133- and CD133+ cells exhibited similar tumorigenicity. However, SP isolated cells, appear to be enriched with tumorigenic stem-like cells capable of generating palpable tumors. BioMed Central 2011-09-14 /pmc/articles/PMC3180433/ /pubmed/21917149 http://dx.doi.org/10.1186/1476-4598-10-112 Text en Copyright ©2011 Zhou et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Zhou, Jianjun Wang, Honghe Cannon, Virginetta Wolcott, Karen Marie Song, Hongbin Yates, Clayton Side population rather than CD133(+ )cells distinguishes enriched tumorigenicity in hTERT-immortalized primary prostate cancer cells |
title | Side population rather than CD133(+ )cells distinguishes enriched tumorigenicity in hTERT-immortalized primary prostate cancer cells |
title_full | Side population rather than CD133(+ )cells distinguishes enriched tumorigenicity in hTERT-immortalized primary prostate cancer cells |
title_fullStr | Side population rather than CD133(+ )cells distinguishes enriched tumorigenicity in hTERT-immortalized primary prostate cancer cells |
title_full_unstemmed | Side population rather than CD133(+ )cells distinguishes enriched tumorigenicity in hTERT-immortalized primary prostate cancer cells |
title_short | Side population rather than CD133(+ )cells distinguishes enriched tumorigenicity in hTERT-immortalized primary prostate cancer cells |
title_sort | side population rather than cd133(+ )cells distinguishes enriched tumorigenicity in htert-immortalized primary prostate cancer cells |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3180433/ https://www.ncbi.nlm.nih.gov/pubmed/21917149 http://dx.doi.org/10.1186/1476-4598-10-112 |
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