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Plasma Lithography Surface Patterning for Creation of Cell Networks

Systematic manipulation of a cell microenvironment with micro- and nanoscale resolution is often required for deciphering various cellular and molecular phenomena. To address this requirement, we have developed a plasma lithography technique to manipulate the cellular microenvironment by creating a...

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Autores principales: Junkin, Michael, Leung, Siu Ling, Yang, Yongliang, Lu, Yi, Volmering, Justin, Wong, Pak Kin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MyJove Corporation 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3197071/
https://www.ncbi.nlm.nih.gov/pubmed/21694697
http://dx.doi.org/10.3791/3115
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author Junkin, Michael
Leung, Siu Ling
Yang, Yongliang
Lu, Yi
Volmering, Justin
Wong, Pak Kin
author_facet Junkin, Michael
Leung, Siu Ling
Yang, Yongliang
Lu, Yi
Volmering, Justin
Wong, Pak Kin
author_sort Junkin, Michael
collection PubMed
description Systematic manipulation of a cell microenvironment with micro- and nanoscale resolution is often required for deciphering various cellular and molecular phenomena. To address this requirement, we have developed a plasma lithography technique to manipulate the cellular microenvironment by creating a patterned surface with feature sizes ranging from 100 nm to millimeters. The goal of this technique is to be able to study, in a controlled way, the behaviors of individual cells as well as groups of cells and their interactions. This plasma lithography method is based on selective modification of the surface chemistry on a substrate by means of shielding the contact of low-temperature plasma with a physical mold. This selective shielding leaves a chemical pattern which can guide cell attachment and movement. This pattern, or surface template, can then be used to create networks of cells whose structure can mimic that found in nature and produces a controllable environment for experimental investigations. The technique is well suited to studying biological phenomenon as it produces stable surface patterns on transparent polymeric substrates in a biocompatible manner. The surface patterns last for weeks to months and can thus guide interaction with cells for long time periods which facilitates the study of long-term cellular processes, such as differentiation and adaption. The modification to the surface is primarily chemical in nature and thus does not introduce topographical or physical interference for interpretation of results. It also does not involve any harsh or toxic substances to achieve patterning and is compatible for tissue culture. Furthermore, it can be applied to modify various types of polymeric substrates, which due to the ability to tune their properties are ideal for and are widely used in biological applications. The resolution achievable is also beneficial, as isolation of specific processes such as migration, adhesion, or binding allows for discrete, clear observations at the single to multicell level. This method has been employed to form diverse networks of different cell types for investigations involving migration, signaling, tissue formation, and the behavior and interactions of neurons arraigned in a network.
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spelling pubmed-31970712011-11-01 Plasma Lithography Surface Patterning for Creation of Cell Networks Junkin, Michael Leung, Siu Ling Yang, Yongliang Lu, Yi Volmering, Justin Wong, Pak Kin J Vis Exp Bioengineering Systematic manipulation of a cell microenvironment with micro- and nanoscale resolution is often required for deciphering various cellular and molecular phenomena. To address this requirement, we have developed a plasma lithography technique to manipulate the cellular microenvironment by creating a patterned surface with feature sizes ranging from 100 nm to millimeters. The goal of this technique is to be able to study, in a controlled way, the behaviors of individual cells as well as groups of cells and their interactions. This plasma lithography method is based on selective modification of the surface chemistry on a substrate by means of shielding the contact of low-temperature plasma with a physical mold. This selective shielding leaves a chemical pattern which can guide cell attachment and movement. This pattern, or surface template, can then be used to create networks of cells whose structure can mimic that found in nature and produces a controllable environment for experimental investigations. The technique is well suited to studying biological phenomenon as it produces stable surface patterns on transparent polymeric substrates in a biocompatible manner. The surface patterns last for weeks to months and can thus guide interaction with cells for long time periods which facilitates the study of long-term cellular processes, such as differentiation and adaption. The modification to the surface is primarily chemical in nature and thus does not introduce topographical or physical interference for interpretation of results. It also does not involve any harsh or toxic substances to achieve patterning and is compatible for tissue culture. Furthermore, it can be applied to modify various types of polymeric substrates, which due to the ability to tune their properties are ideal for and are widely used in biological applications. The resolution achievable is also beneficial, as isolation of specific processes such as migration, adhesion, or binding allows for discrete, clear observations at the single to multicell level. This method has been employed to form diverse networks of different cell types for investigations involving migration, signaling, tissue formation, and the behavior and interactions of neurons arraigned in a network. MyJove Corporation 2011-06-14 /pmc/articles/PMC3197071/ /pubmed/21694697 http://dx.doi.org/10.3791/3115 Text en Copyright © 2011, Journal of Visualized Experiments http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. To view a copy of this license, visithttp://creativecommons.org/licenses/by-nc-nd/3.0/
spellingShingle Bioengineering
Junkin, Michael
Leung, Siu Ling
Yang, Yongliang
Lu, Yi
Volmering, Justin
Wong, Pak Kin
Plasma Lithography Surface Patterning for Creation of Cell Networks
title Plasma Lithography Surface Patterning for Creation of Cell Networks
title_full Plasma Lithography Surface Patterning for Creation of Cell Networks
title_fullStr Plasma Lithography Surface Patterning for Creation of Cell Networks
title_full_unstemmed Plasma Lithography Surface Patterning for Creation of Cell Networks
title_short Plasma Lithography Surface Patterning for Creation of Cell Networks
title_sort plasma lithography surface patterning for creation of cell networks
topic Bioengineering
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3197071/
https://www.ncbi.nlm.nih.gov/pubmed/21694697
http://dx.doi.org/10.3791/3115
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