Necdin modulates proliferative cell survival of human cells in response to radiation-induced genotoxic stress

BACKGROUND: The finite replicative lifespan of cells, termed cellular senescence, has been proposed as a protective mechanism against the proliferation of oncogenically damaged cells, that fuel cancer. This concept is further supported by the induction of premature senescence, a process which is act...

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Autores principales: Lafontaine, Julie, Tchakarska, Guergana, Rodier, Francis, Mes-Masson, Anne-Marie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3495902/
https://www.ncbi.nlm.nih.gov/pubmed/22691188
http://dx.doi.org/10.1186/1471-2407-12-234
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author Lafontaine, Julie
Tchakarska, Guergana
Rodier, Francis
Mes-Masson, Anne-Marie
author_facet Lafontaine, Julie
Tchakarska, Guergana
Rodier, Francis
Mes-Masson, Anne-Marie
author_sort Lafontaine, Julie
collection PubMed
description BACKGROUND: The finite replicative lifespan of cells, termed cellular senescence, has been proposed as a protective mechanism against the proliferation of oncogenically damaged cells, that fuel cancer. This concept is further supported by the induction of premature senescence, a process which is activated when an oncogene is expressed in normal primary cells as well as following intense genotoxic stresses. Thus, deregulation of genes that control this process, like the tumor suppressor p53, may contribute to promoting cancer by allowing cells to bypass senescence. A better understanding of the genes that contribute to the establishment of senescence is therefore warranted. Necdin interacts with p53 and is also a p53 target gene, although the importance of Necdin in the p53 response is not clearly understood. METHODS: In this study, we first investigated Necdin protein expression during replicative senescence and premature senescence induced by gamma irradiation and by the overexpression of oncogenic RasV12. Gain and loss of function experiments were used to evaluate the contribution of Necdin during the senescence process. RESULTS: Necdin expression declined during replicative aging of IMR90 primary human fibroblasts or following induction of premature senescence. Decrease in Necdin expression seemed to be a consequence of the establishment of senescence since the depletion of Necdin in human cells did not induce a senescence-like growth arrest nor a flat morphology or SA-β-galactosidase activity normally associated with senescence. Similarly, overexpression of Necdin did not affect the life span of IMR90 cells. However, we demonstrate that in normal human cells, Necdin expression mimicked the effect of p53 inactivation by increasing radioresistance. CONCLUSION: This result suggests that Necdin potentially attenuate p53 signaling in response to genotoxic stress in human cells and supports similar results describing an inhibitory function of Necdin over p53-dependent growth arrest in mice.
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spelling pubmed-34959022012-11-13 Necdin modulates proliferative cell survival of human cells in response to radiation-induced genotoxic stress Lafontaine, Julie Tchakarska, Guergana Rodier, Francis Mes-Masson, Anne-Marie BMC Cancer Research Article BACKGROUND: The finite replicative lifespan of cells, termed cellular senescence, has been proposed as a protective mechanism against the proliferation of oncogenically damaged cells, that fuel cancer. This concept is further supported by the induction of premature senescence, a process which is activated when an oncogene is expressed in normal primary cells as well as following intense genotoxic stresses. Thus, deregulation of genes that control this process, like the tumor suppressor p53, may contribute to promoting cancer by allowing cells to bypass senescence. A better understanding of the genes that contribute to the establishment of senescence is therefore warranted. Necdin interacts with p53 and is also a p53 target gene, although the importance of Necdin in the p53 response is not clearly understood. METHODS: In this study, we first investigated Necdin protein expression during replicative senescence and premature senescence induced by gamma irradiation and by the overexpression of oncogenic RasV12. Gain and loss of function experiments were used to evaluate the contribution of Necdin during the senescence process. RESULTS: Necdin expression declined during replicative aging of IMR90 primary human fibroblasts or following induction of premature senescence. Decrease in Necdin expression seemed to be a consequence of the establishment of senescence since the depletion of Necdin in human cells did not induce a senescence-like growth arrest nor a flat morphology or SA-β-galactosidase activity normally associated with senescence. Similarly, overexpression of Necdin did not affect the life span of IMR90 cells. However, we demonstrate that in normal human cells, Necdin expression mimicked the effect of p53 inactivation by increasing radioresistance. CONCLUSION: This result suggests that Necdin potentially attenuate p53 signaling in response to genotoxic stress in human cells and supports similar results describing an inhibitory function of Necdin over p53-dependent growth arrest in mice. BioMed Central 2012-06-12 /pmc/articles/PMC3495902/ /pubmed/22691188 http://dx.doi.org/10.1186/1471-2407-12-234 Text en Copyright ©2012 Lafontaine et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Lafontaine, Julie
Tchakarska, Guergana
Rodier, Francis
Mes-Masson, Anne-Marie
Necdin modulates proliferative cell survival of human cells in response to radiation-induced genotoxic stress
title Necdin modulates proliferative cell survival of human cells in response to radiation-induced genotoxic stress
title_full Necdin modulates proliferative cell survival of human cells in response to radiation-induced genotoxic stress
title_fullStr Necdin modulates proliferative cell survival of human cells in response to radiation-induced genotoxic stress
title_full_unstemmed Necdin modulates proliferative cell survival of human cells in response to radiation-induced genotoxic stress
title_short Necdin modulates proliferative cell survival of human cells in response to radiation-induced genotoxic stress
title_sort necdin modulates proliferative cell survival of human cells in response to radiation-induced genotoxic stress
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3495902/
https://www.ncbi.nlm.nih.gov/pubmed/22691188
http://dx.doi.org/10.1186/1471-2407-12-234
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