Persistent androgen receptor-mediated transcription in castration-resistant prostate cancer under androgen-deprived conditions

The androgen receptor (AR) is a ligand-inducible transcription factor that mediates androgen action in target tissues. Upon ligand binding, the AR binds to thousands of genomic loci and activates a cell-type specific gene program. Prostate cancer growth and progression depend on androgen-induced AR...

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Autores principales: Decker, Keith F., Zheng, Dali, He, Yuhong, Bowman, Tamara, Edwards, John R., Jia, Li
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3510497/
https://www.ncbi.nlm.nih.gov/pubmed/23019221
http://dx.doi.org/10.1093/nar/gks888
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author Decker, Keith F.
Zheng, Dali
He, Yuhong
Bowman, Tamara
Edwards, John R.
Jia, Li
author_facet Decker, Keith F.
Zheng, Dali
He, Yuhong
Bowman, Tamara
Edwards, John R.
Jia, Li
author_sort Decker, Keith F.
collection PubMed
description The androgen receptor (AR) is a ligand-inducible transcription factor that mediates androgen action in target tissues. Upon ligand binding, the AR binds to thousands of genomic loci and activates a cell-type specific gene program. Prostate cancer growth and progression depend on androgen-induced AR signaling. Treatment of advanced prostate cancer through medical or surgical castration leads to initial response and durable remission, but resistance inevitably develops. In castration-resistant prostate cancer (CRPC), AR activity remains critical for tumor growth despite androgen deprivation. Although previous studies have focused on ligand-dependent AR signaling, in this study we explore AR function under the androgen-deprived conditions characteristic of CRPC. Our data demonstrate that AR persistently occupies a distinct set of genomic loci after androgen deprivation in CRPC. These androgen-independent AR occupied regions have constitutively open chromatin structures that lack the canonical androgen response element and are independent of FoxA1, a transcription factor involved in ligand-dependent AR targeting. Many AR binding events occur at proximal promoters, which can act as enhancers to augment transcriptional activities of other promoters through DNA looping. We further show that androgen-independent AR binding directs a gene expression program in CRPC, which is necessary for the growth of CRPC after androgen withdrawal.
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spelling pubmed-35104972012-11-30 Persistent androgen receptor-mediated transcription in castration-resistant prostate cancer under androgen-deprived conditions Decker, Keith F. Zheng, Dali He, Yuhong Bowman, Tamara Edwards, John R. Jia, Li Nucleic Acids Res Gene Regulation, Chromatin and Epigenetics The androgen receptor (AR) is a ligand-inducible transcription factor that mediates androgen action in target tissues. Upon ligand binding, the AR binds to thousands of genomic loci and activates a cell-type specific gene program. Prostate cancer growth and progression depend on androgen-induced AR signaling. Treatment of advanced prostate cancer through medical or surgical castration leads to initial response and durable remission, but resistance inevitably develops. In castration-resistant prostate cancer (CRPC), AR activity remains critical for tumor growth despite androgen deprivation. Although previous studies have focused on ligand-dependent AR signaling, in this study we explore AR function under the androgen-deprived conditions characteristic of CRPC. Our data demonstrate that AR persistently occupies a distinct set of genomic loci after androgen deprivation in CRPC. These androgen-independent AR occupied regions have constitutively open chromatin structures that lack the canonical androgen response element and are independent of FoxA1, a transcription factor involved in ligand-dependent AR targeting. Many AR binding events occur at proximal promoters, which can act as enhancers to augment transcriptional activities of other promoters through DNA looping. We further show that androgen-independent AR binding directs a gene expression program in CRPC, which is necessary for the growth of CRPC after androgen withdrawal. Oxford University Press 2012-11 2012-09-27 /pmc/articles/PMC3510497/ /pubmed/23019221 http://dx.doi.org/10.1093/nar/gks888 Text en © The Author(s) 2012. Published by Oxford University Press. http://creativecommons.org/licenses/by/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Gene Regulation, Chromatin and Epigenetics
Decker, Keith F.
Zheng, Dali
He, Yuhong
Bowman, Tamara
Edwards, John R.
Jia, Li
Persistent androgen receptor-mediated transcription in castration-resistant prostate cancer under androgen-deprived conditions
title Persistent androgen receptor-mediated transcription in castration-resistant prostate cancer under androgen-deprived conditions
title_full Persistent androgen receptor-mediated transcription in castration-resistant prostate cancer under androgen-deprived conditions
title_fullStr Persistent androgen receptor-mediated transcription in castration-resistant prostate cancer under androgen-deprived conditions
title_full_unstemmed Persistent androgen receptor-mediated transcription in castration-resistant prostate cancer under androgen-deprived conditions
title_short Persistent androgen receptor-mediated transcription in castration-resistant prostate cancer under androgen-deprived conditions
title_sort persistent androgen receptor-mediated transcription in castration-resistant prostate cancer under androgen-deprived conditions
topic Gene Regulation, Chromatin and Epigenetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3510497/
https://www.ncbi.nlm.nih.gov/pubmed/23019221
http://dx.doi.org/10.1093/nar/gks888
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