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Elements of Transcriptional Machinery Are Compatible among Plants and Mammals

In the present work, the objective has been to analyse the compatibility of plant and human transcriptional machinery. The experiments revealed that nuclear import and export are conserved among plants and mammals. Further it has been shown that transactivation of a human promoter occurs by human tr...

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Autores principales: Wolf, Annette, Akrap, Nina, Marg, Berenice, Galliardt, Helena, Heiligentag, Martyna, Humpert, Fabian, Sauer, Markus, Kaltschmidt, Barbara, Kaltschmidt, Christian, Seidel, Thorsten
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3543382/
https://www.ncbi.nlm.nih.gov/pubmed/23326494
http://dx.doi.org/10.1371/journal.pone.0053737
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author Wolf, Annette
Akrap, Nina
Marg, Berenice
Galliardt, Helena
Heiligentag, Martyna
Humpert, Fabian
Sauer, Markus
Kaltschmidt, Barbara
Kaltschmidt, Christian
Seidel, Thorsten
author_facet Wolf, Annette
Akrap, Nina
Marg, Berenice
Galliardt, Helena
Heiligentag, Martyna
Humpert, Fabian
Sauer, Markus
Kaltschmidt, Barbara
Kaltschmidt, Christian
Seidel, Thorsten
author_sort Wolf, Annette
collection PubMed
description In the present work, the objective has been to analyse the compatibility of plant and human transcriptional machinery. The experiments revealed that nuclear import and export are conserved among plants and mammals. Further it has been shown that transactivation of a human promoter occurs by human transcription factor NF-κB in plant cells, demonstrating that the transcriptional machinery is highly conserved in both kingdoms. Functionality was also seen for regulatory elements of NF-κB such as its inhibitor IκB isoform α that negatively regulated the transactivation activity of the p50/RelA heterodimer by interaction with NF-κB in plant cells. Nuclear export of RelA could be demonstrated by FRAP-measurements so that RelA shows nucleo-cytoplasmic shuttling as reported for RelA in mammalian cells. The data reveals the high level of compatibility of human transcriptional elements with the plant transcriptional machinery. Thus, Arabidopsis thaliana mesophyll protoplasts might provide a new heterologous expression system for the investigation of the human NF-κB signaling pathways. The system successfully enabled the controlled manipulation of NF-κB activity. We suggest the plant protoplast system as a tool for reconstitution and analyses of mammalian pathways and for direct observation of responses to e.g. pharmaceuticals. The major advantage of the system is the absence of interference with endogenous factors that affect and crosstalk with the pathway.
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spelling pubmed-35433822013-01-16 Elements of Transcriptional Machinery Are Compatible among Plants and Mammals Wolf, Annette Akrap, Nina Marg, Berenice Galliardt, Helena Heiligentag, Martyna Humpert, Fabian Sauer, Markus Kaltschmidt, Barbara Kaltschmidt, Christian Seidel, Thorsten PLoS One Research Article In the present work, the objective has been to analyse the compatibility of plant and human transcriptional machinery. The experiments revealed that nuclear import and export are conserved among plants and mammals. Further it has been shown that transactivation of a human promoter occurs by human transcription factor NF-κB in plant cells, demonstrating that the transcriptional machinery is highly conserved in both kingdoms. Functionality was also seen for regulatory elements of NF-κB such as its inhibitor IκB isoform α that negatively regulated the transactivation activity of the p50/RelA heterodimer by interaction with NF-κB in plant cells. Nuclear export of RelA could be demonstrated by FRAP-measurements so that RelA shows nucleo-cytoplasmic shuttling as reported for RelA in mammalian cells. The data reveals the high level of compatibility of human transcriptional elements with the plant transcriptional machinery. Thus, Arabidopsis thaliana mesophyll protoplasts might provide a new heterologous expression system for the investigation of the human NF-κB signaling pathways. The system successfully enabled the controlled manipulation of NF-κB activity. We suggest the plant protoplast system as a tool for reconstitution and analyses of mammalian pathways and for direct observation of responses to e.g. pharmaceuticals. The major advantage of the system is the absence of interference with endogenous factors that affect and crosstalk with the pathway. Public Library of Science 2013-01-11 /pmc/articles/PMC3543382/ /pubmed/23326494 http://dx.doi.org/10.1371/journal.pone.0053737 Text en © 2013 Wolf et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Wolf, Annette
Akrap, Nina
Marg, Berenice
Galliardt, Helena
Heiligentag, Martyna
Humpert, Fabian
Sauer, Markus
Kaltschmidt, Barbara
Kaltschmidt, Christian
Seidel, Thorsten
Elements of Transcriptional Machinery Are Compatible among Plants and Mammals
title Elements of Transcriptional Machinery Are Compatible among Plants and Mammals
title_full Elements of Transcriptional Machinery Are Compatible among Plants and Mammals
title_fullStr Elements of Transcriptional Machinery Are Compatible among Plants and Mammals
title_full_unstemmed Elements of Transcriptional Machinery Are Compatible among Plants and Mammals
title_short Elements of Transcriptional Machinery Are Compatible among Plants and Mammals
title_sort elements of transcriptional machinery are compatible among plants and mammals
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3543382/
https://www.ncbi.nlm.nih.gov/pubmed/23326494
http://dx.doi.org/10.1371/journal.pone.0053737
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