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RNA–Mediated Epigenetic Heredity Requires the Cytosine Methyltransferase Dnmt2

RNA–mediated transmission of phenotypes is an important way to explain non-Mendelian heredity. We have previously shown that small non-coding RNAs can induce hereditary epigenetic variations in mice and act as the transgenerational signalling molecules. Two prominent examples for these paramutations...

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Autores principales: Kiani, Jafar, Grandjean, Valérie, Liebers, Reinhard, Tuorto, Francesca, Ghanbarian, Hossein, Lyko, Frank, Cuzin, François, Rassoulzadegan, Minoo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3662642/
https://www.ncbi.nlm.nih.gov/pubmed/23717211
http://dx.doi.org/10.1371/journal.pgen.1003498
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author Kiani, Jafar
Grandjean, Valérie
Liebers, Reinhard
Tuorto, Francesca
Ghanbarian, Hossein
Lyko, Frank
Cuzin, François
Rassoulzadegan, Minoo
author_facet Kiani, Jafar
Grandjean, Valérie
Liebers, Reinhard
Tuorto, Francesca
Ghanbarian, Hossein
Lyko, Frank
Cuzin, François
Rassoulzadegan, Minoo
author_sort Kiani, Jafar
collection PubMed
description RNA–mediated transmission of phenotypes is an important way to explain non-Mendelian heredity. We have previously shown that small non-coding RNAs can induce hereditary epigenetic variations in mice and act as the transgenerational signalling molecules. Two prominent examples for these paramutations include the epigenetic modulation of the Kit gene, resulting in altered fur coloration, and the modulation of the Sox9 gene, resulting in an overgrowth phenotype. We now report that expression of the Dnmt2 RNA methyltransferase is required for the establishment and hereditary maintenance of both paramutations. Our data show that the Kit paramutant phenotype was not transmitted to the progeny of Dnmt2(−/−) mice and that the Sox9 paramutation was also not established in Dnmt2(−/−) embryos. Similarly, RNA from Dnmt2-negative Kit heterozygotes did not induce the paramutant phenotype when microinjected into Dnmt2-deficient fertilized eggs and microinjection of the miR-124 microRNA failed to induce the characteristic giant phenotype. In agreement with an RNA–mediated mechanism of inheritance, no change was observed in the DNA methylation profiles of the Kit locus between the wild-type and paramutant mice. RNA bisulfite sequencing confirmed Dnmt2-dependent tRNA methylation in mouse sperm and also indicated Dnmt2-dependent cytosine methylation in Kit RNA in paramutant embryos. Together, these findings uncover a novel function of Dnmt2 in RNA–mediated epigenetic heredity.
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spelling pubmed-36626422013-05-28 RNA–Mediated Epigenetic Heredity Requires the Cytosine Methyltransferase Dnmt2 Kiani, Jafar Grandjean, Valérie Liebers, Reinhard Tuorto, Francesca Ghanbarian, Hossein Lyko, Frank Cuzin, François Rassoulzadegan, Minoo PLoS Genet Research Article RNA–mediated transmission of phenotypes is an important way to explain non-Mendelian heredity. We have previously shown that small non-coding RNAs can induce hereditary epigenetic variations in mice and act as the transgenerational signalling molecules. Two prominent examples for these paramutations include the epigenetic modulation of the Kit gene, resulting in altered fur coloration, and the modulation of the Sox9 gene, resulting in an overgrowth phenotype. We now report that expression of the Dnmt2 RNA methyltransferase is required for the establishment and hereditary maintenance of both paramutations. Our data show that the Kit paramutant phenotype was not transmitted to the progeny of Dnmt2(−/−) mice and that the Sox9 paramutation was also not established in Dnmt2(−/−) embryos. Similarly, RNA from Dnmt2-negative Kit heterozygotes did not induce the paramutant phenotype when microinjected into Dnmt2-deficient fertilized eggs and microinjection of the miR-124 microRNA failed to induce the characteristic giant phenotype. In agreement with an RNA–mediated mechanism of inheritance, no change was observed in the DNA methylation profiles of the Kit locus between the wild-type and paramutant mice. RNA bisulfite sequencing confirmed Dnmt2-dependent tRNA methylation in mouse sperm and also indicated Dnmt2-dependent cytosine methylation in Kit RNA in paramutant embryos. Together, these findings uncover a novel function of Dnmt2 in RNA–mediated epigenetic heredity. Public Library of Science 2013-05-23 /pmc/articles/PMC3662642/ /pubmed/23717211 http://dx.doi.org/10.1371/journal.pgen.1003498 Text en © 2013 Kiani et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Kiani, Jafar
Grandjean, Valérie
Liebers, Reinhard
Tuorto, Francesca
Ghanbarian, Hossein
Lyko, Frank
Cuzin, François
Rassoulzadegan, Minoo
RNA–Mediated Epigenetic Heredity Requires the Cytosine Methyltransferase Dnmt2
title RNA–Mediated Epigenetic Heredity Requires the Cytosine Methyltransferase Dnmt2
title_full RNA–Mediated Epigenetic Heredity Requires the Cytosine Methyltransferase Dnmt2
title_fullStr RNA–Mediated Epigenetic Heredity Requires the Cytosine Methyltransferase Dnmt2
title_full_unstemmed RNA–Mediated Epigenetic Heredity Requires the Cytosine Methyltransferase Dnmt2
title_short RNA–Mediated Epigenetic Heredity Requires the Cytosine Methyltransferase Dnmt2
title_sort rna–mediated epigenetic heredity requires the cytosine methyltransferase dnmt2
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3662642/
https://www.ncbi.nlm.nih.gov/pubmed/23717211
http://dx.doi.org/10.1371/journal.pgen.1003498
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