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Concomitant production of two proteases and alpha-amylase by a novel strain of Bacillus subtilis in a microprocessor controlled bioreactor
We describe the simultaneous production of Bacillus subtilis based proteases and alpha amylase using a computer controlled laboratory scale 7.5 L batch bioreactor. The present strain is the first to be reported that concomitantly produces these two industrially important enzymes. The growth and spor...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Sociedade Brasileira de Microbiologia
2012
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3768893/ https://www.ncbi.nlm.nih.gov/pubmed/24031930 http://dx.doi.org/10.1590/S1517-838220120003000033 |
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author | Mukhtar, Hamid Ikram-ul-Haq, |
author_facet | Mukhtar, Hamid Ikram-ul-Haq, |
author_sort | Mukhtar, Hamid |
collection | PubMed |
description | We describe the simultaneous production of Bacillus subtilis based proteases and alpha amylase using a computer controlled laboratory scale 7.5 L batch bioreactor. The present strain is the first to be reported that concomitantly produces these two industrially important enzymes. The growth and sporulation of Bacillus subtilis was monitored and maximum production of alkaline protease and alpha amylase was found to coincide with maximum sporulation. Two types of proteases were detected in the fermentation broth; a neutral and an alkaline protease most active in a pH range of 7.0–8.0 and 8.0–10, respectively. Maximum production of proteases was observed at an incubation temperature of 37°C while that of alpha amylase was observed at 40°C. The optimum aeration and agitation levels for protease production were 0.6 L/L/min and 200rpm, respectively, and for alpha amylase were 0.6 L/L/min and 150 rpm. The kinetic parameters Y(p/x) and qp were also found to be significant at the given fermentation conditions. |
format | Online Article Text |
id | pubmed-3768893 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Sociedade Brasileira de Microbiologia |
record_format | MEDLINE/PubMed |
spelling | pubmed-37688932013-09-12 Concomitant production of two proteases and alpha-amylase by a novel strain of Bacillus subtilis in a microprocessor controlled bioreactor Mukhtar, Hamid Ikram-ul-Haq, Braz J Microbiol Industrial Microbiology We describe the simultaneous production of Bacillus subtilis based proteases and alpha amylase using a computer controlled laboratory scale 7.5 L batch bioreactor. The present strain is the first to be reported that concomitantly produces these two industrially important enzymes. The growth and sporulation of Bacillus subtilis was monitored and maximum production of alkaline protease and alpha amylase was found to coincide with maximum sporulation. Two types of proteases were detected in the fermentation broth; a neutral and an alkaline protease most active in a pH range of 7.0–8.0 and 8.0–10, respectively. Maximum production of proteases was observed at an incubation temperature of 37°C while that of alpha amylase was observed at 40°C. The optimum aeration and agitation levels for protease production were 0.6 L/L/min and 200rpm, respectively, and for alpha amylase were 0.6 L/L/min and 150 rpm. The kinetic parameters Y(p/x) and qp were also found to be significant at the given fermentation conditions. Sociedade Brasileira de Microbiologia 2012 2012-06-01 /pmc/articles/PMC3768893/ /pubmed/24031930 http://dx.doi.org/10.1590/S1517-838220120003000033 Text en © Sociedade Brasileira de Microbiologia http://creativecommons.org/licenses/by-nc/3.0/ All the content of the journal, except where otherwise noted, is licensed under a Creative Commons License |
spellingShingle | Industrial Microbiology Mukhtar, Hamid Ikram-ul-Haq, Concomitant production of two proteases and alpha-amylase by a novel strain of Bacillus subtilis in a microprocessor controlled bioreactor |
title | Concomitant production of two proteases and alpha-amylase by a novel strain of Bacillus subtilis in a microprocessor controlled bioreactor |
title_full | Concomitant production of two proteases and alpha-amylase by a novel strain of Bacillus subtilis in a microprocessor controlled bioreactor |
title_fullStr | Concomitant production of two proteases and alpha-amylase by a novel strain of Bacillus subtilis in a microprocessor controlled bioreactor |
title_full_unstemmed | Concomitant production of two proteases and alpha-amylase by a novel strain of Bacillus subtilis in a microprocessor controlled bioreactor |
title_short | Concomitant production of two proteases and alpha-amylase by a novel strain of Bacillus subtilis in a microprocessor controlled bioreactor |
title_sort | concomitant production of two proteases and alpha-amylase by a novel strain of bacillus subtilis in a microprocessor controlled bioreactor |
topic | Industrial Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3768893/ https://www.ncbi.nlm.nih.gov/pubmed/24031930 http://dx.doi.org/10.1590/S1517-838220120003000033 |
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