ITE and TCDD Differentially Regulate the Vascular Remodeling of Rat Placenta via the Activation of AhR

Vascular remodeling in the placenta is essential for normal fetal development. The previous studies have demonstrated that in utero exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD, an environmental toxicant) induces the intrauterine fetal death in many species via the activation of aryl hydroc...

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Autores principales: Wu, Yanming, Chen, Xiao, Zhou, Qian, He, Qizhi, Kang, Jiuhong, Zheng, Jing, Wang, Kai, Duan, Tao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3901702/
https://www.ncbi.nlm.nih.gov/pubmed/24475139
http://dx.doi.org/10.1371/journal.pone.0086549
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author Wu, Yanming
Chen, Xiao
Zhou, Qian
He, Qizhi
Kang, Jiuhong
Zheng, Jing
Wang, Kai
Duan, Tao
author_facet Wu, Yanming
Chen, Xiao
Zhou, Qian
He, Qizhi
Kang, Jiuhong
Zheng, Jing
Wang, Kai
Duan, Tao
author_sort Wu, Yanming
collection PubMed
description Vascular remodeling in the placenta is essential for normal fetal development. The previous studies have demonstrated that in utero exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD, an environmental toxicant) induces the intrauterine fetal death in many species via the activation of aryl hydrocarbon receptor (AhR). In the current study, we compared the effects of 2-(1′H-indole-3′-carbonyl)-thiazole-4-carboxylic acid methyl ester (ITE) and TCDD on the vascular remodeling of rat placentas. Pregnant rats on gestational day (GD) 15 were randomly assigned into 5 groups, and were exposed to a single dose of 1.6 and 8.0 mg/kg body weight (bw) ITE, 1.6 and 8.0 µg/kg bw TCDD, or an equivalent volume of the vehicle, respectively. The dams were sacrificed on GD20 and the placental tissues were gathered. The intrauterine fetal death was observed only in 8.0 µg/kg bw TCDD-exposed group and no significant difference was seen in either the placental weight or the fetal weight among all these groups. The immunohistochemical and histological analyses revealed that as compared with the vehicle-control, TCDD, but not ITE, suppressed the placental vascular remodeling, including reduced the ratio of the placental labyrinth zone to the basal zone thickness (at least 0.71 fold of control), inhibited the maternal sinusoids dilation and thickened the trophoblastic septa. However, no marked difference was observed in the density of fetal capillaries in the labyrinth zone among these groups, although significant differences were detected in the expression of angiogenic growth factors between ITE and TCDD-exposed groups, especially Angiopoietin-2 (Ang-2), Endoglin, Interferon-γ (IFN-γ) and placenta growth factor (PIGF). These results suggest ITE and TCDD differentially regulate the vascular remodeling of rat placentas, as well as the expression of angiogenic factors and their receptors, which in turn may alter the blood flow in the late gestation and partially resulted in intrauterine fetal death.
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spelling pubmed-39017022014-01-28 ITE and TCDD Differentially Regulate the Vascular Remodeling of Rat Placenta via the Activation of AhR Wu, Yanming Chen, Xiao Zhou, Qian He, Qizhi Kang, Jiuhong Zheng, Jing Wang, Kai Duan, Tao PLoS One Research Article Vascular remodeling in the placenta is essential for normal fetal development. The previous studies have demonstrated that in utero exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD, an environmental toxicant) induces the intrauterine fetal death in many species via the activation of aryl hydrocarbon receptor (AhR). In the current study, we compared the effects of 2-(1′H-indole-3′-carbonyl)-thiazole-4-carboxylic acid methyl ester (ITE) and TCDD on the vascular remodeling of rat placentas. Pregnant rats on gestational day (GD) 15 were randomly assigned into 5 groups, and were exposed to a single dose of 1.6 and 8.0 mg/kg body weight (bw) ITE, 1.6 and 8.0 µg/kg bw TCDD, or an equivalent volume of the vehicle, respectively. The dams were sacrificed on GD20 and the placental tissues were gathered. The intrauterine fetal death was observed only in 8.0 µg/kg bw TCDD-exposed group and no significant difference was seen in either the placental weight or the fetal weight among all these groups. The immunohistochemical and histological analyses revealed that as compared with the vehicle-control, TCDD, but not ITE, suppressed the placental vascular remodeling, including reduced the ratio of the placental labyrinth zone to the basal zone thickness (at least 0.71 fold of control), inhibited the maternal sinusoids dilation and thickened the trophoblastic septa. However, no marked difference was observed in the density of fetal capillaries in the labyrinth zone among these groups, although significant differences were detected in the expression of angiogenic growth factors between ITE and TCDD-exposed groups, especially Angiopoietin-2 (Ang-2), Endoglin, Interferon-γ (IFN-γ) and placenta growth factor (PIGF). These results suggest ITE and TCDD differentially regulate the vascular remodeling of rat placentas, as well as the expression of angiogenic factors and their receptors, which in turn may alter the blood flow in the late gestation and partially resulted in intrauterine fetal death. Public Library of Science 2014-01-24 /pmc/articles/PMC3901702/ /pubmed/24475139 http://dx.doi.org/10.1371/journal.pone.0086549 Text en © 2014 Wu et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Wu, Yanming
Chen, Xiao
Zhou, Qian
He, Qizhi
Kang, Jiuhong
Zheng, Jing
Wang, Kai
Duan, Tao
ITE and TCDD Differentially Regulate the Vascular Remodeling of Rat Placenta via the Activation of AhR
title ITE and TCDD Differentially Regulate the Vascular Remodeling of Rat Placenta via the Activation of AhR
title_full ITE and TCDD Differentially Regulate the Vascular Remodeling of Rat Placenta via the Activation of AhR
title_fullStr ITE and TCDD Differentially Regulate the Vascular Remodeling of Rat Placenta via the Activation of AhR
title_full_unstemmed ITE and TCDD Differentially Regulate the Vascular Remodeling of Rat Placenta via the Activation of AhR
title_short ITE and TCDD Differentially Regulate the Vascular Remodeling of Rat Placenta via the Activation of AhR
title_sort ite and tcdd differentially regulate the vascular remodeling of rat placenta via the activation of ahr
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3901702/
https://www.ncbi.nlm.nih.gov/pubmed/24475139
http://dx.doi.org/10.1371/journal.pone.0086549
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