Kraken: A set of tools for quality control and analysis of high-throughput sequence data()
New sequencing technologies pose significant challenges in terms of data complexity and magnitude. It is essential that efficient software is developed with performance that scales with this growth in sequence information. Here we present a comprehensive and integrated set of tools for the analysis...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Academic Press
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3991327/ https://www.ncbi.nlm.nih.gov/pubmed/23816787 http://dx.doi.org/10.1016/j.ymeth.2013.06.027 |
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author | Davis, Matthew P.A. van Dongen, Stijn Abreu-Goodger, Cei Bartonicek, Nenad Enright, Anton J. |
author_facet | Davis, Matthew P.A. van Dongen, Stijn Abreu-Goodger, Cei Bartonicek, Nenad Enright, Anton J. |
author_sort | Davis, Matthew P.A. |
collection | PubMed |
description | New sequencing technologies pose significant challenges in terms of data complexity and magnitude. It is essential that efficient software is developed with performance that scales with this growth in sequence information. Here we present a comprehensive and integrated set of tools for the analysis of data from large scale sequencing experiments. It supports adapter detection and removal, demultiplexing of barcodes, paired-end data, a range of read architectures and the efficient removal of sequence redundancy. Sequences can be trimmed and filtered based on length, quality and complexity. Quality control plots track sequence length, composition and summary statistics with respect to genomic annotation. Several use cases have been integrated into a single streamlined pipeline, including both mRNA and small RNA sequencing experiments. This pipeline interfaces with existing tools for genomic mapping and differential expression analysis. |
format | Online Article Text |
id | pubmed-3991327 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Academic Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-39913272014-04-18 Kraken: A set of tools for quality control and analysis of high-throughput sequence data() Davis, Matthew P.A. van Dongen, Stijn Abreu-Goodger, Cei Bartonicek, Nenad Enright, Anton J. Methods Article New sequencing technologies pose significant challenges in terms of data complexity and magnitude. It is essential that efficient software is developed with performance that scales with this growth in sequence information. Here we present a comprehensive and integrated set of tools for the analysis of data from large scale sequencing experiments. It supports adapter detection and removal, demultiplexing of barcodes, paired-end data, a range of read architectures and the efficient removal of sequence redundancy. Sequences can be trimmed and filtered based on length, quality and complexity. Quality control plots track sequence length, composition and summary statistics with respect to genomic annotation. Several use cases have been integrated into a single streamlined pipeline, including both mRNA and small RNA sequencing experiments. This pipeline interfaces with existing tools for genomic mapping and differential expression analysis. Academic Press 2013-09-01 /pmc/articles/PMC3991327/ /pubmed/23816787 http://dx.doi.org/10.1016/j.ymeth.2013.06.027 Text en © 2013 The Authors http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/3.0/). |
spellingShingle | Article Davis, Matthew P.A. van Dongen, Stijn Abreu-Goodger, Cei Bartonicek, Nenad Enright, Anton J. Kraken: A set of tools for quality control and analysis of high-throughput sequence data() |
title | Kraken: A set of tools for quality control and analysis of high-throughput sequence data() |
title_full | Kraken: A set of tools for quality control and analysis of high-throughput sequence data() |
title_fullStr | Kraken: A set of tools for quality control and analysis of high-throughput sequence data() |
title_full_unstemmed | Kraken: A set of tools for quality control and analysis of high-throughput sequence data() |
title_short | Kraken: A set of tools for quality control and analysis of high-throughput sequence data() |
title_sort | kraken: a set of tools for quality control and analysis of high-throughput sequence data() |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3991327/ https://www.ncbi.nlm.nih.gov/pubmed/23816787 http://dx.doi.org/10.1016/j.ymeth.2013.06.027 |
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