Viruses and Tetraspanins: Lessons from Single Molecule Approaches

Tetraspanins are four-span membrane proteins that are widely distributed in multi-cellular organisms and involved in several infectious diseases. They have the unique property to form a network of protein-protein interaction within the plasma membrane, due to the lateral associations with one anothe...

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Autores principales: Dahmane, Selma, Rubinstein, Eric, Milhiet, Pierre-Emmanuel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4036545/
https://www.ncbi.nlm.nih.gov/pubmed/24800676
http://dx.doi.org/10.3390/v6051992
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author Dahmane, Selma
Rubinstein, Eric
Milhiet, Pierre-Emmanuel
author_facet Dahmane, Selma
Rubinstein, Eric
Milhiet, Pierre-Emmanuel
author_sort Dahmane, Selma
collection PubMed
description Tetraspanins are four-span membrane proteins that are widely distributed in multi-cellular organisms and involved in several infectious diseases. They have the unique property to form a network of protein-protein interaction within the plasma membrane, due to the lateral associations with one another and with other membrane proteins. Tracking tetraspanins at the single molecule level using fluorescence microscopy has revealed the membrane behavior of the tetraspanins CD9 and CD81 in epithelial cell lines, providing a first dynamic view of this network. Single molecule tracking highlighted that these 2 proteins can freely diffuse within the plasma membrane but can also be trapped, permanently or transiently, in tetraspanin-enriched areas. More recently, a similar strategy has been used to investigate tetraspanin membrane behavior in the context of human immunodeficiency virus type 1 (HIV-1) and hepatitis C virus (HCV) infection. In this review we summarize the main results emphasizing the relationship in terms of membrane partitioning between tetraspanins, some of their partners such as Claudin-1 and EWI-2, and viral proteins during infection. These results will be analyzed in the context of other membrane microdomains, stressing the difference between raft and tetraspanin-enriched microdomains, but also in comparison with virus diffusion at the cell surface. New advanced single molecule techniques that could help to further explore tetraspanin assemblies will be also discussed.
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spelling pubmed-40365452014-05-28 Viruses and Tetraspanins: Lessons from Single Molecule Approaches Dahmane, Selma Rubinstein, Eric Milhiet, Pierre-Emmanuel Viruses Review Tetraspanins are four-span membrane proteins that are widely distributed in multi-cellular organisms and involved in several infectious diseases. They have the unique property to form a network of protein-protein interaction within the plasma membrane, due to the lateral associations with one another and with other membrane proteins. Tracking tetraspanins at the single molecule level using fluorescence microscopy has revealed the membrane behavior of the tetraspanins CD9 and CD81 in epithelial cell lines, providing a first dynamic view of this network. Single molecule tracking highlighted that these 2 proteins can freely diffuse within the plasma membrane but can also be trapped, permanently or transiently, in tetraspanin-enriched areas. More recently, a similar strategy has been used to investigate tetraspanin membrane behavior in the context of human immunodeficiency virus type 1 (HIV-1) and hepatitis C virus (HCV) infection. In this review we summarize the main results emphasizing the relationship in terms of membrane partitioning between tetraspanins, some of their partners such as Claudin-1 and EWI-2, and viral proteins during infection. These results will be analyzed in the context of other membrane microdomains, stressing the difference between raft and tetraspanin-enriched microdomains, but also in comparison with virus diffusion at the cell surface. New advanced single molecule techniques that could help to further explore tetraspanin assemblies will be also discussed. MDPI 2014-05-05 /pmc/articles/PMC4036545/ /pubmed/24800676 http://dx.doi.org/10.3390/v6051992 Text en © 2014 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Review
Dahmane, Selma
Rubinstein, Eric
Milhiet, Pierre-Emmanuel
Viruses and Tetraspanins: Lessons from Single Molecule Approaches
title Viruses and Tetraspanins: Lessons from Single Molecule Approaches
title_full Viruses and Tetraspanins: Lessons from Single Molecule Approaches
title_fullStr Viruses and Tetraspanins: Lessons from Single Molecule Approaches
title_full_unstemmed Viruses and Tetraspanins: Lessons from Single Molecule Approaches
title_short Viruses and Tetraspanins: Lessons from Single Molecule Approaches
title_sort viruses and tetraspanins: lessons from single molecule approaches
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4036545/
https://www.ncbi.nlm.nih.gov/pubmed/24800676
http://dx.doi.org/10.3390/v6051992
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