Dual Effects of Hydrogen Sulfide Donor on Meiosis and Cumulus Expansion of Porcine Cumulus-Oocyte Complexes
Hydrogen sulfide (H(2)S) has been revealed to be a signal molecule with second messenger action in the somatic cells of many tissues, including the reproductive tract. The aim of this study was to address how exogenous H(2)S acts on the meiotic maturation of porcine oocytes, including key maturation...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Public Library of Science
2014
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4077697/ https://www.ncbi.nlm.nih.gov/pubmed/24984032 http://dx.doi.org/10.1371/journal.pone.0099613 |
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author | Nevoral, Jan Petr, Jaroslav Gelaude, Armance Bodart, Jean-Francois Kucerova-Chrpova, Veronika Sedmikova, Marketa Krejcova, Tereza Kolbabova, Tereza Dvorakova, Marketa Vyskocilova, Alena Weingartova, Ivona Krivohlavkova, Lenka Zalmanova, Tereza Jilek, Frantisek |
author_facet | Nevoral, Jan Petr, Jaroslav Gelaude, Armance Bodart, Jean-Francois Kucerova-Chrpova, Veronika Sedmikova, Marketa Krejcova, Tereza Kolbabova, Tereza Dvorakova, Marketa Vyskocilova, Alena Weingartova, Ivona Krivohlavkova, Lenka Zalmanova, Tereza Jilek, Frantisek |
author_sort | Nevoral, Jan |
collection | PubMed |
description | Hydrogen sulfide (H(2)S) has been revealed to be a signal molecule with second messenger action in the somatic cells of many tissues, including the reproductive tract. The aim of this study was to address how exogenous H(2)S acts on the meiotic maturation of porcine oocytes, including key maturation factors such as MPF and MAPK, and cumulus expansion intensity of cumulus-oocyte complexes. We observed that the H(2)S donor, Na(2)S, accelerated oocyte in vitro maturation in a dose-dependent manner, following an increase of MPF activity around germinal vesicle breakdown. Concurrently, the H(2)S donor affected cumulus expansion, monitored by hyaluronic acid production. Our results suggest that the H(2)S donor influences oocyte maturation and thus also participates in the regulation of cumulus expansion. The exogenous H(2)S donor apparently affects key signal pathways of oocyte maturation and cumulus expansion, resulting in faster oocyte maturation with little need of cumulus expansion. |
format | Online Article Text |
id | pubmed-4077697 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-40776972014-07-03 Dual Effects of Hydrogen Sulfide Donor on Meiosis and Cumulus Expansion of Porcine Cumulus-Oocyte Complexes Nevoral, Jan Petr, Jaroslav Gelaude, Armance Bodart, Jean-Francois Kucerova-Chrpova, Veronika Sedmikova, Marketa Krejcova, Tereza Kolbabova, Tereza Dvorakova, Marketa Vyskocilova, Alena Weingartova, Ivona Krivohlavkova, Lenka Zalmanova, Tereza Jilek, Frantisek PLoS One Research Article Hydrogen sulfide (H(2)S) has been revealed to be a signal molecule with second messenger action in the somatic cells of many tissues, including the reproductive tract. The aim of this study was to address how exogenous H(2)S acts on the meiotic maturation of porcine oocytes, including key maturation factors such as MPF and MAPK, and cumulus expansion intensity of cumulus-oocyte complexes. We observed that the H(2)S donor, Na(2)S, accelerated oocyte in vitro maturation in a dose-dependent manner, following an increase of MPF activity around germinal vesicle breakdown. Concurrently, the H(2)S donor affected cumulus expansion, monitored by hyaluronic acid production. Our results suggest that the H(2)S donor influences oocyte maturation and thus also participates in the regulation of cumulus expansion. The exogenous H(2)S donor apparently affects key signal pathways of oocyte maturation and cumulus expansion, resulting in faster oocyte maturation with little need of cumulus expansion. Public Library of Science 2014-07-01 /pmc/articles/PMC4077697/ /pubmed/24984032 http://dx.doi.org/10.1371/journal.pone.0099613 Text en © 2014 Nevoral et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Nevoral, Jan Petr, Jaroslav Gelaude, Armance Bodart, Jean-Francois Kucerova-Chrpova, Veronika Sedmikova, Marketa Krejcova, Tereza Kolbabova, Tereza Dvorakova, Marketa Vyskocilova, Alena Weingartova, Ivona Krivohlavkova, Lenka Zalmanova, Tereza Jilek, Frantisek Dual Effects of Hydrogen Sulfide Donor on Meiosis and Cumulus Expansion of Porcine Cumulus-Oocyte Complexes |
title | Dual Effects of Hydrogen Sulfide Donor on Meiosis and Cumulus Expansion of Porcine Cumulus-Oocyte Complexes |
title_full | Dual Effects of Hydrogen Sulfide Donor on Meiosis and Cumulus Expansion of Porcine Cumulus-Oocyte Complexes |
title_fullStr | Dual Effects of Hydrogen Sulfide Donor on Meiosis and Cumulus Expansion of Porcine Cumulus-Oocyte Complexes |
title_full_unstemmed | Dual Effects of Hydrogen Sulfide Donor on Meiosis and Cumulus Expansion of Porcine Cumulus-Oocyte Complexes |
title_short | Dual Effects of Hydrogen Sulfide Donor on Meiosis and Cumulus Expansion of Porcine Cumulus-Oocyte Complexes |
title_sort | dual effects of hydrogen sulfide donor on meiosis and cumulus expansion of porcine cumulus-oocyte complexes |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4077697/ https://www.ncbi.nlm.nih.gov/pubmed/24984032 http://dx.doi.org/10.1371/journal.pone.0099613 |
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