Dual Effects of Hydrogen Sulfide Donor on Meiosis and Cumulus Expansion of Porcine Cumulus-Oocyte Complexes

Hydrogen sulfide (H(2)S) has been revealed to be a signal molecule with second messenger action in the somatic cells of many tissues, including the reproductive tract. The aim of this study was to address how exogenous H(2)S acts on the meiotic maturation of porcine oocytes, including key maturation...

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Autores principales: Nevoral, Jan, Petr, Jaroslav, Gelaude, Armance, Bodart, Jean-Francois, Kucerova-Chrpova, Veronika, Sedmikova, Marketa, Krejcova, Tereza, Kolbabova, Tereza, Dvorakova, Marketa, Vyskocilova, Alena, Weingartova, Ivona, Krivohlavkova, Lenka, Zalmanova, Tereza, Jilek, Frantisek
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4077697/
https://www.ncbi.nlm.nih.gov/pubmed/24984032
http://dx.doi.org/10.1371/journal.pone.0099613
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author Nevoral, Jan
Petr, Jaroslav
Gelaude, Armance
Bodart, Jean-Francois
Kucerova-Chrpova, Veronika
Sedmikova, Marketa
Krejcova, Tereza
Kolbabova, Tereza
Dvorakova, Marketa
Vyskocilova, Alena
Weingartova, Ivona
Krivohlavkova, Lenka
Zalmanova, Tereza
Jilek, Frantisek
author_facet Nevoral, Jan
Petr, Jaroslav
Gelaude, Armance
Bodart, Jean-Francois
Kucerova-Chrpova, Veronika
Sedmikova, Marketa
Krejcova, Tereza
Kolbabova, Tereza
Dvorakova, Marketa
Vyskocilova, Alena
Weingartova, Ivona
Krivohlavkova, Lenka
Zalmanova, Tereza
Jilek, Frantisek
author_sort Nevoral, Jan
collection PubMed
description Hydrogen sulfide (H(2)S) has been revealed to be a signal molecule with second messenger action in the somatic cells of many tissues, including the reproductive tract. The aim of this study was to address how exogenous H(2)S acts on the meiotic maturation of porcine oocytes, including key maturation factors such as MPF and MAPK, and cumulus expansion intensity of cumulus-oocyte complexes. We observed that the H(2)S donor, Na(2)S, accelerated oocyte in vitro maturation in a dose-dependent manner, following an increase of MPF activity around germinal vesicle breakdown. Concurrently, the H(2)S donor affected cumulus expansion, monitored by hyaluronic acid production. Our results suggest that the H(2)S donor influences oocyte maturation and thus also participates in the regulation of cumulus expansion. The exogenous H(2)S donor apparently affects key signal pathways of oocyte maturation and cumulus expansion, resulting in faster oocyte maturation with little need of cumulus expansion.
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spelling pubmed-40776972014-07-03 Dual Effects of Hydrogen Sulfide Donor on Meiosis and Cumulus Expansion of Porcine Cumulus-Oocyte Complexes Nevoral, Jan Petr, Jaroslav Gelaude, Armance Bodart, Jean-Francois Kucerova-Chrpova, Veronika Sedmikova, Marketa Krejcova, Tereza Kolbabova, Tereza Dvorakova, Marketa Vyskocilova, Alena Weingartova, Ivona Krivohlavkova, Lenka Zalmanova, Tereza Jilek, Frantisek PLoS One Research Article Hydrogen sulfide (H(2)S) has been revealed to be a signal molecule with second messenger action in the somatic cells of many tissues, including the reproductive tract. The aim of this study was to address how exogenous H(2)S acts on the meiotic maturation of porcine oocytes, including key maturation factors such as MPF and MAPK, and cumulus expansion intensity of cumulus-oocyte complexes. We observed that the H(2)S donor, Na(2)S, accelerated oocyte in vitro maturation in a dose-dependent manner, following an increase of MPF activity around germinal vesicle breakdown. Concurrently, the H(2)S donor affected cumulus expansion, monitored by hyaluronic acid production. Our results suggest that the H(2)S donor influences oocyte maturation and thus also participates in the regulation of cumulus expansion. The exogenous H(2)S donor apparently affects key signal pathways of oocyte maturation and cumulus expansion, resulting in faster oocyte maturation with little need of cumulus expansion. Public Library of Science 2014-07-01 /pmc/articles/PMC4077697/ /pubmed/24984032 http://dx.doi.org/10.1371/journal.pone.0099613 Text en © 2014 Nevoral et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Nevoral, Jan
Petr, Jaroslav
Gelaude, Armance
Bodart, Jean-Francois
Kucerova-Chrpova, Veronika
Sedmikova, Marketa
Krejcova, Tereza
Kolbabova, Tereza
Dvorakova, Marketa
Vyskocilova, Alena
Weingartova, Ivona
Krivohlavkova, Lenka
Zalmanova, Tereza
Jilek, Frantisek
Dual Effects of Hydrogen Sulfide Donor on Meiosis and Cumulus Expansion of Porcine Cumulus-Oocyte Complexes
title Dual Effects of Hydrogen Sulfide Donor on Meiosis and Cumulus Expansion of Porcine Cumulus-Oocyte Complexes
title_full Dual Effects of Hydrogen Sulfide Donor on Meiosis and Cumulus Expansion of Porcine Cumulus-Oocyte Complexes
title_fullStr Dual Effects of Hydrogen Sulfide Donor on Meiosis and Cumulus Expansion of Porcine Cumulus-Oocyte Complexes
title_full_unstemmed Dual Effects of Hydrogen Sulfide Donor on Meiosis and Cumulus Expansion of Porcine Cumulus-Oocyte Complexes
title_short Dual Effects of Hydrogen Sulfide Donor on Meiosis and Cumulus Expansion of Porcine Cumulus-Oocyte Complexes
title_sort dual effects of hydrogen sulfide donor on meiosis and cumulus expansion of porcine cumulus-oocyte complexes
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4077697/
https://www.ncbi.nlm.nih.gov/pubmed/24984032
http://dx.doi.org/10.1371/journal.pone.0099613
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