Microfluidic control of axonal guidance
The precision of axonal pathfinding and the accurate formation of functional neural circuitry are crucial for an organism during development as well as during adult central and peripheral nerve regeneration. While chemical cues are believed to be primarily responsible for axonal pathfinding, we hypo...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4185387/ https://www.ncbi.nlm.nih.gov/pubmed/25283077 http://dx.doi.org/10.1038/srep06457 |
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author | Gu, Ling Black, Bryan Ordonez, Simon Mondal, Argha Jain, Ankur Mohanty, Samarendra |
author_facet | Gu, Ling Black, Bryan Ordonez, Simon Mondal, Argha Jain, Ankur Mohanty, Samarendra |
author_sort | Gu, Ling |
collection | PubMed |
description | The precision of axonal pathfinding and the accurate formation of functional neural circuitry are crucial for an organism during development as well as during adult central and peripheral nerve regeneration. While chemical cues are believed to be primarily responsible for axonal pathfinding, we hypothesize that forces due to localized fluid flow may directly affect neuronal guidance during early organ development. Here, we report direct evidence of fluid flow influencing axonal migration, producing turning angles of up to 90°. Microfluidic flow simulations indicate that an axon may experience significant bending force due to cross-flow, which may contribute to the observed axonal turning. This method of flow-based guidance was successfully used to fasciculate one advancing axon onto another, showcasing the potential of this technique to be used for the formation of in vitro neuronal circuits. |
format | Online Article Text |
id | pubmed-4185387 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-41853872014-10-17 Microfluidic control of axonal guidance Gu, Ling Black, Bryan Ordonez, Simon Mondal, Argha Jain, Ankur Mohanty, Samarendra Sci Rep Article The precision of axonal pathfinding and the accurate formation of functional neural circuitry are crucial for an organism during development as well as during adult central and peripheral nerve regeneration. While chemical cues are believed to be primarily responsible for axonal pathfinding, we hypothesize that forces due to localized fluid flow may directly affect neuronal guidance during early organ development. Here, we report direct evidence of fluid flow influencing axonal migration, producing turning angles of up to 90°. Microfluidic flow simulations indicate that an axon may experience significant bending force due to cross-flow, which may contribute to the observed axonal turning. This method of flow-based guidance was successfully used to fasciculate one advancing axon onto another, showcasing the potential of this technique to be used for the formation of in vitro neuronal circuits. Nature Publishing Group 2014-10-06 /pmc/articles/PMC4185387/ /pubmed/25283077 http://dx.doi.org/10.1038/srep06457 Text en Copyright © 2014, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by-nc-sa/4.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/4.0/ |
spellingShingle | Article Gu, Ling Black, Bryan Ordonez, Simon Mondal, Argha Jain, Ankur Mohanty, Samarendra Microfluidic control of axonal guidance |
title | Microfluidic control of axonal guidance |
title_full | Microfluidic control of axonal guidance |
title_fullStr | Microfluidic control of axonal guidance |
title_full_unstemmed | Microfluidic control of axonal guidance |
title_short | Microfluidic control of axonal guidance |
title_sort | microfluidic control of axonal guidance |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4185387/ https://www.ncbi.nlm.nih.gov/pubmed/25283077 http://dx.doi.org/10.1038/srep06457 |
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