Licensing of Yeast Centrosome Duplication Requires Phosphoregulation of Sfi1

Duplication of centrosomes once per cell cycle is essential for bipolar spindle formation and genome maintenance and is controlled in part by cyclin-dependent kinases (Cdks). Our study identifies Sfi1, a conserved component of centrosomes, as the first Cdk substrate required to restrict centrosome d...

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Autores principales: Avena, Jennifer S., Burns, Shannon, Yu, Zulin, Ebmeier, Christopher C., Old, William M., Jaspersen, Sue L., Winey, Mark
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4207612/
https://www.ncbi.nlm.nih.gov/pubmed/25340401
http://dx.doi.org/10.1371/journal.pgen.1004666
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author Avena, Jennifer S.
Burns, Shannon
Yu, Zulin
Ebmeier, Christopher C.
Old, William M.
Jaspersen, Sue L.
Winey, Mark
author_facet Avena, Jennifer S.
Burns, Shannon
Yu, Zulin
Ebmeier, Christopher C.
Old, William M.
Jaspersen, Sue L.
Winey, Mark
author_sort Avena, Jennifer S.
collection PubMed
description Duplication of centrosomes once per cell cycle is essential for bipolar spindle formation and genome maintenance and is controlled in part by cyclin-dependent kinases (Cdks). Our study identifies Sfi1, a conserved component of centrosomes, as the first Cdk substrate required to restrict centrosome duplication to once per cell cycle. We found that reducing Cdk1 phosphorylation by changing Sfi1 phosphorylation sites to nonphosphorylatable residues leads to defects in separation of duplicated spindle pole bodies (SPBs, yeast centrosomes) and to inappropriate SPB reduplication during mitosis. These cells also display defects in bipolar spindle assembly, chromosome segregation, and growth. Our findings lead to a model whereby phosphoregulation of Sfi1 by Cdk1 has the dual function of promoting SPB separation for spindle formation and preventing premature SPB duplication. In addition, we provide evidence that the protein phosphatase Cdc14 has the converse role of activating licensing, likely via dephosphorylation of Sfi1.
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spelling pubmed-42076122014-10-27 Licensing of Yeast Centrosome Duplication Requires Phosphoregulation of Sfi1 Avena, Jennifer S. Burns, Shannon Yu, Zulin Ebmeier, Christopher C. Old, William M. Jaspersen, Sue L. Winey, Mark PLoS Genet Research Article Duplication of centrosomes once per cell cycle is essential for bipolar spindle formation and genome maintenance and is controlled in part by cyclin-dependent kinases (Cdks). Our study identifies Sfi1, a conserved component of centrosomes, as the first Cdk substrate required to restrict centrosome duplication to once per cell cycle. We found that reducing Cdk1 phosphorylation by changing Sfi1 phosphorylation sites to nonphosphorylatable residues leads to defects in separation of duplicated spindle pole bodies (SPBs, yeast centrosomes) and to inappropriate SPB reduplication during mitosis. These cells also display defects in bipolar spindle assembly, chromosome segregation, and growth. Our findings lead to a model whereby phosphoregulation of Sfi1 by Cdk1 has the dual function of promoting SPB separation for spindle formation and preventing premature SPB duplication. In addition, we provide evidence that the protein phosphatase Cdc14 has the converse role of activating licensing, likely via dephosphorylation of Sfi1. Public Library of Science 2014-10-23 /pmc/articles/PMC4207612/ /pubmed/25340401 http://dx.doi.org/10.1371/journal.pgen.1004666 Text en © 2014 Avena et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Avena, Jennifer S.
Burns, Shannon
Yu, Zulin
Ebmeier, Christopher C.
Old, William M.
Jaspersen, Sue L.
Winey, Mark
Licensing of Yeast Centrosome Duplication Requires Phosphoregulation of Sfi1
title Licensing of Yeast Centrosome Duplication Requires Phosphoregulation of Sfi1
title_full Licensing of Yeast Centrosome Duplication Requires Phosphoregulation of Sfi1
title_fullStr Licensing of Yeast Centrosome Duplication Requires Phosphoregulation of Sfi1
title_full_unstemmed Licensing of Yeast Centrosome Duplication Requires Phosphoregulation of Sfi1
title_short Licensing of Yeast Centrosome Duplication Requires Phosphoregulation of Sfi1
title_sort licensing of yeast centrosome duplication requires phosphoregulation of sfi1
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4207612/
https://www.ncbi.nlm.nih.gov/pubmed/25340401
http://dx.doi.org/10.1371/journal.pgen.1004666
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