Licensing of Yeast Centrosome Duplication Requires Phosphoregulation of Sfi1
Duplication of centrosomes once per cell cycle is essential for bipolar spindle formation and genome maintenance and is controlled in part by cyclin-dependent kinases (Cdks). Our study identifies Sfi1, a conserved component of centrosomes, as the first Cdk substrate required to restrict centrosome d...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4207612/ https://www.ncbi.nlm.nih.gov/pubmed/25340401 http://dx.doi.org/10.1371/journal.pgen.1004666 |
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author | Avena, Jennifer S. Burns, Shannon Yu, Zulin Ebmeier, Christopher C. Old, William M. Jaspersen, Sue L. Winey, Mark |
author_facet | Avena, Jennifer S. Burns, Shannon Yu, Zulin Ebmeier, Christopher C. Old, William M. Jaspersen, Sue L. Winey, Mark |
author_sort | Avena, Jennifer S. |
collection | PubMed |
description | Duplication of centrosomes once per cell cycle is essential for bipolar spindle formation and genome maintenance and is controlled in part by cyclin-dependent kinases (Cdks). Our study identifies Sfi1, a conserved component of centrosomes, as the first Cdk substrate required to restrict centrosome duplication to once per cell cycle. We found that reducing Cdk1 phosphorylation by changing Sfi1 phosphorylation sites to nonphosphorylatable residues leads to defects in separation of duplicated spindle pole bodies (SPBs, yeast centrosomes) and to inappropriate SPB reduplication during mitosis. These cells also display defects in bipolar spindle assembly, chromosome segregation, and growth. Our findings lead to a model whereby phosphoregulation of Sfi1 by Cdk1 has the dual function of promoting SPB separation for spindle formation and preventing premature SPB duplication. In addition, we provide evidence that the protein phosphatase Cdc14 has the converse role of activating licensing, likely via dephosphorylation of Sfi1. |
format | Online Article Text |
id | pubmed-4207612 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-42076122014-10-27 Licensing of Yeast Centrosome Duplication Requires Phosphoregulation of Sfi1 Avena, Jennifer S. Burns, Shannon Yu, Zulin Ebmeier, Christopher C. Old, William M. Jaspersen, Sue L. Winey, Mark PLoS Genet Research Article Duplication of centrosomes once per cell cycle is essential for bipolar spindle formation and genome maintenance and is controlled in part by cyclin-dependent kinases (Cdks). Our study identifies Sfi1, a conserved component of centrosomes, as the first Cdk substrate required to restrict centrosome duplication to once per cell cycle. We found that reducing Cdk1 phosphorylation by changing Sfi1 phosphorylation sites to nonphosphorylatable residues leads to defects in separation of duplicated spindle pole bodies (SPBs, yeast centrosomes) and to inappropriate SPB reduplication during mitosis. These cells also display defects in bipolar spindle assembly, chromosome segregation, and growth. Our findings lead to a model whereby phosphoregulation of Sfi1 by Cdk1 has the dual function of promoting SPB separation for spindle formation and preventing premature SPB duplication. In addition, we provide evidence that the protein phosphatase Cdc14 has the converse role of activating licensing, likely via dephosphorylation of Sfi1. Public Library of Science 2014-10-23 /pmc/articles/PMC4207612/ /pubmed/25340401 http://dx.doi.org/10.1371/journal.pgen.1004666 Text en © 2014 Avena et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Avena, Jennifer S. Burns, Shannon Yu, Zulin Ebmeier, Christopher C. Old, William M. Jaspersen, Sue L. Winey, Mark Licensing of Yeast Centrosome Duplication Requires Phosphoregulation of Sfi1 |
title | Licensing of Yeast Centrosome Duplication Requires Phosphoregulation of Sfi1 |
title_full | Licensing of Yeast Centrosome Duplication Requires Phosphoregulation of Sfi1 |
title_fullStr | Licensing of Yeast Centrosome Duplication Requires Phosphoregulation of Sfi1 |
title_full_unstemmed | Licensing of Yeast Centrosome Duplication Requires Phosphoregulation of Sfi1 |
title_short | Licensing of Yeast Centrosome Duplication Requires Phosphoregulation of Sfi1 |
title_sort | licensing of yeast centrosome duplication requires phosphoregulation of sfi1 |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4207612/ https://www.ncbi.nlm.nih.gov/pubmed/25340401 http://dx.doi.org/10.1371/journal.pgen.1004666 |
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