In vitro biological screening of the anticholinesterase and antiproliferative activities of medicinal plants belonging to Annonaceae

The aim of this research was to investigate the antiproliferative and anticholinesterase activities of 11 extracts from 5 Annonaceae species in vitro. Antiproliferative activity was assessed using 10 human cancer cell lines. Thin-layer chromatography and a microplate assay were used to screen the ex...

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Autores principales: Formagio, A.S.N., Vieira, M.C., Volobuff, C.R.F., Silva, M.S., Matos, A.I., Cardoso, C.A.L., Foglio, M.A., Carvalho, J.E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Associação Brasileira de Divulgação Científica 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4418360/
https://www.ncbi.nlm.nih.gov/pubmed/25714885
http://dx.doi.org/10.1590/1414-431X20144127
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author Formagio, A.S.N.
Vieira, M.C.
Volobuff, C.R.F.
Silva, M.S.
Matos, A.I.
Cardoso, C.A.L.
Foglio, M.A.
Carvalho, J.E.
author_facet Formagio, A.S.N.
Vieira, M.C.
Volobuff, C.R.F.
Silva, M.S.
Matos, A.I.
Cardoso, C.A.L.
Foglio, M.A.
Carvalho, J.E.
author_sort Formagio, A.S.N.
collection PubMed
description The aim of this research was to investigate the antiproliferative and anticholinesterase activities of 11 extracts from 5 Annonaceae species in vitro. Antiproliferative activity was assessed using 10 human cancer cell lines. Thin-layer chromatography and a microplate assay were used to screen the extracts for acetylcholinesterase (AchE) inhibitors using Ellman's reagent. The chemical compositions of the active extracts were investigated using high performance liquid chromatography. Eleven extracts obtained from five Annonaceae plant species were active and were particularly effective against the UA251, NCI-470 lung, HT-29, NCI/ADR, and K-562 cell lines with growth inhibition (GI(50)) values of 0.04-0.06, 0.02-0.50, 0.01-0.12, 0.10-0.27, and 0.02-0.04 µg/mL, respectively. In addition, the Annona crassiflora and A. coriacea seed extracts were the most active among the tested extracts and the most effective against the tumor cell lines, with GI(50) values below 8.90 µg/mL. The A. cacans extract displayed the lowest activity. Based on the microplate assay, the percent AchE inhibition of the extracts ranged from 12 to 52%, and the A. coriacea seed extract resulted in the greatest inhibition (52%). Caffeic acid, sinapic acid, and rutin were present at higher concentrations in the A. crassiflora seed samples. The A. coriacea seeds contained ferulic and sinapic acid. Overall, the results indicated that A. crassiflora and A. coriacea extracts have antiproliferative and anticholinesterase properties, which opens up new possibilities for alternative pharmacotherapy drugs.
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spelling pubmed-44183602015-05-15 In vitro biological screening of the anticholinesterase and antiproliferative activities of medicinal plants belonging to Annonaceae Formagio, A.S.N. Vieira, M.C. Volobuff, C.R.F. Silva, M.S. Matos, A.I. Cardoso, C.A.L. Foglio, M.A. Carvalho, J.E. Braz J Med Biol Res Biomedical Sciences The aim of this research was to investigate the antiproliferative and anticholinesterase activities of 11 extracts from 5 Annonaceae species in vitro. Antiproliferative activity was assessed using 10 human cancer cell lines. Thin-layer chromatography and a microplate assay were used to screen the extracts for acetylcholinesterase (AchE) inhibitors using Ellman's reagent. The chemical compositions of the active extracts were investigated using high performance liquid chromatography. Eleven extracts obtained from five Annonaceae plant species were active and were particularly effective against the UA251, NCI-470 lung, HT-29, NCI/ADR, and K-562 cell lines with growth inhibition (GI(50)) values of 0.04-0.06, 0.02-0.50, 0.01-0.12, 0.10-0.27, and 0.02-0.04 µg/mL, respectively. In addition, the Annona crassiflora and A. coriacea seed extracts were the most active among the tested extracts and the most effective against the tumor cell lines, with GI(50) values below 8.90 µg/mL. The A. cacans extract displayed the lowest activity. Based on the microplate assay, the percent AchE inhibition of the extracts ranged from 12 to 52%, and the A. coriacea seed extract resulted in the greatest inhibition (52%). Caffeic acid, sinapic acid, and rutin were present at higher concentrations in the A. crassiflora seed samples. The A. coriacea seeds contained ferulic and sinapic acid. Overall, the results indicated that A. crassiflora and A. coriacea extracts have antiproliferative and anticholinesterase properties, which opens up new possibilities for alternative pharmacotherapy drugs. Associação Brasileira de Divulgação Científica 2015-02-13 /pmc/articles/PMC4418360/ /pubmed/25714885 http://dx.doi.org/10.1590/1414-431X20144127 Text en http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Biomedical Sciences
Formagio, A.S.N.
Vieira, M.C.
Volobuff, C.R.F.
Silva, M.S.
Matos, A.I.
Cardoso, C.A.L.
Foglio, M.A.
Carvalho, J.E.
In vitro biological screening of the anticholinesterase and antiproliferative activities of medicinal plants belonging to Annonaceae
title In vitro biological screening of the anticholinesterase and antiproliferative activities of medicinal plants belonging to Annonaceae
title_full In vitro biological screening of the anticholinesterase and antiproliferative activities of medicinal plants belonging to Annonaceae
title_fullStr In vitro biological screening of the anticholinesterase and antiproliferative activities of medicinal plants belonging to Annonaceae
title_full_unstemmed In vitro biological screening of the anticholinesterase and antiproliferative activities of medicinal plants belonging to Annonaceae
title_short In vitro biological screening of the anticholinesterase and antiproliferative activities of medicinal plants belonging to Annonaceae
title_sort in vitro biological screening of the anticholinesterase and antiproliferative activities of medicinal plants belonging to annonaceae
topic Biomedical Sciences
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4418360/
https://www.ncbi.nlm.nih.gov/pubmed/25714885
http://dx.doi.org/10.1590/1414-431X20144127
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