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Production of recombinant VP1-derived virus-like particles from novel human polyomaviruses in yeast

BACKGROUND: Eleven new human polyomaviruses (HPyVs) have been identified in the last decade. Serological studies show that these novel HPyVs sub-clinically infect humans at an early age. The routes of infection, entry pathways, and cell tropism of new HPyVs remain unknown. VP1 proteins of polyomavir...

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Autores principales: Norkiene, Milda, Stonyte, Jomante, Ziogiene, Danguole, Mazeike, Egle, Sasnauskas, Kestutis, Gedvilaite, Alma
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4523907/
https://www.ncbi.nlm.nih.gov/pubmed/26239840
http://dx.doi.org/10.1186/s12896-015-0187-z
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author Norkiene, Milda
Stonyte, Jomante
Ziogiene, Danguole
Mazeike, Egle
Sasnauskas, Kestutis
Gedvilaite, Alma
author_facet Norkiene, Milda
Stonyte, Jomante
Ziogiene, Danguole
Mazeike, Egle
Sasnauskas, Kestutis
Gedvilaite, Alma
author_sort Norkiene, Milda
collection PubMed
description BACKGROUND: Eleven new human polyomaviruses (HPyVs) have been identified in the last decade. Serological studies show that these novel HPyVs sub-clinically infect humans at an early age. The routes of infection, entry pathways, and cell tropism of new HPyVs remain unknown. VP1 proteins of polyomaviruses can assembly into virus-like particles (VLPs). As cell culturing systems for HPyV are currently not available, VP1-derived VLPs may be useful tools in basic research and biotechnological applications. RESULTS: Recombinant VP1-derived VLPs from 11 newly identified HPyVs were efficiently expressed in yeast. VP1 proteins derived from Merkel cell polyomavirus (MCPyV), trichodysplasia spinulosa-associated polyomavirus (TSPyV), and New Jersey polyomavirus (NJPyV) self-assembled into homogeneous similarly-sized VLPs. Karolinska Institutet polyomavirus (KIPyV), HPyV7, HPyV9, HPyV10, and St. Louis polyomavirus (STLPyV) VP1 proteins formed VLPs that varied in size with diameters ranging from 20 to 60 nm. Smaller-sized VLPs (25–35 nm in diameter) predominated in preparations from Washington University polyomavirus (WUPyV) and HPyV6. Attempts to express recombinant HPyV12 VP1-derived VLPs in yeast indicate that translation of VP1 might start at the second of two potential translation initiation sites in the VP1-encoding open reading frame (ORF). This translation resulted in a 364-amino acid-long VP1 protein, which efficiently self-assembled into typical PyV VLPs. MCPyV-, KIPyV-, TSPyV-, HPyV9-, HPyV10-, and HPyV12-derived VLPs showed hemagglutination (HA) assay activity in guinea pig erythrocytes, whereas WUPyV-, HPyV6-, HPyV7-, STLPyV- and NJPyV-derived VP1 VLPs did not. CONCLUSIONS: The yeast expression system was successfully utilized for high-throughput production of recombinant VP1-derived VLPs from 11 newly identified HPyVs. HPyV12 VP1-derived VLPs were generated from the second of two potential translation initiation sites in the VP1-encoding ORF. Recombinant VLPs produced in yeast originated from different HPyVs demonstrated distinct HA activities and may be useful in virus diagnostics, capsid structure studies, or investigation of entry pathways and cell tropism of HPyVs until cell culture systems for new HPyVs are developed.
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spelling pubmed-45239072015-08-05 Production of recombinant VP1-derived virus-like particles from novel human polyomaviruses in yeast Norkiene, Milda Stonyte, Jomante Ziogiene, Danguole Mazeike, Egle Sasnauskas, Kestutis Gedvilaite, Alma BMC Biotechnol Research Article BACKGROUND: Eleven new human polyomaviruses (HPyVs) have been identified in the last decade. Serological studies show that these novel HPyVs sub-clinically infect humans at an early age. The routes of infection, entry pathways, and cell tropism of new HPyVs remain unknown. VP1 proteins of polyomaviruses can assembly into virus-like particles (VLPs). As cell culturing systems for HPyV are currently not available, VP1-derived VLPs may be useful tools in basic research and biotechnological applications. RESULTS: Recombinant VP1-derived VLPs from 11 newly identified HPyVs were efficiently expressed in yeast. VP1 proteins derived from Merkel cell polyomavirus (MCPyV), trichodysplasia spinulosa-associated polyomavirus (TSPyV), and New Jersey polyomavirus (NJPyV) self-assembled into homogeneous similarly-sized VLPs. Karolinska Institutet polyomavirus (KIPyV), HPyV7, HPyV9, HPyV10, and St. Louis polyomavirus (STLPyV) VP1 proteins formed VLPs that varied in size with diameters ranging from 20 to 60 nm. Smaller-sized VLPs (25–35 nm in diameter) predominated in preparations from Washington University polyomavirus (WUPyV) and HPyV6. Attempts to express recombinant HPyV12 VP1-derived VLPs in yeast indicate that translation of VP1 might start at the second of two potential translation initiation sites in the VP1-encoding open reading frame (ORF). This translation resulted in a 364-amino acid-long VP1 protein, which efficiently self-assembled into typical PyV VLPs. MCPyV-, KIPyV-, TSPyV-, HPyV9-, HPyV10-, and HPyV12-derived VLPs showed hemagglutination (HA) assay activity in guinea pig erythrocytes, whereas WUPyV-, HPyV6-, HPyV7-, STLPyV- and NJPyV-derived VP1 VLPs did not. CONCLUSIONS: The yeast expression system was successfully utilized for high-throughput production of recombinant VP1-derived VLPs from 11 newly identified HPyVs. HPyV12 VP1-derived VLPs were generated from the second of two potential translation initiation sites in the VP1-encoding ORF. Recombinant VLPs produced in yeast originated from different HPyVs demonstrated distinct HA activities and may be useful in virus diagnostics, capsid structure studies, or investigation of entry pathways and cell tropism of HPyVs until cell culture systems for new HPyVs are developed. BioMed Central 2015-08-04 /pmc/articles/PMC4523907/ /pubmed/26239840 http://dx.doi.org/10.1186/s12896-015-0187-z Text en © Norkiene et al. 2015 Open Access This article is an article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Norkiene, Milda
Stonyte, Jomante
Ziogiene, Danguole
Mazeike, Egle
Sasnauskas, Kestutis
Gedvilaite, Alma
Production of recombinant VP1-derived virus-like particles from novel human polyomaviruses in yeast
title Production of recombinant VP1-derived virus-like particles from novel human polyomaviruses in yeast
title_full Production of recombinant VP1-derived virus-like particles from novel human polyomaviruses in yeast
title_fullStr Production of recombinant VP1-derived virus-like particles from novel human polyomaviruses in yeast
title_full_unstemmed Production of recombinant VP1-derived virus-like particles from novel human polyomaviruses in yeast
title_short Production of recombinant VP1-derived virus-like particles from novel human polyomaviruses in yeast
title_sort production of recombinant vp1-derived virus-like particles from novel human polyomaviruses in yeast
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4523907/
https://www.ncbi.nlm.nih.gov/pubmed/26239840
http://dx.doi.org/10.1186/s12896-015-0187-z
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