Evaluation of profertility effect of probiotic Lactobacillus plantarum 2621 in a murine model

BACKGROUND & OBJECTIVES: Urogenital infections of bacterial origin have a high incidence among the female population at reproductive age, affecting the fertility. Strains of Escherichia coli can colonize the vagina and replace natural microflora. Lactobacillus the predominant vaginal microorgani...

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Autores principales: Bhandari, Praveen, Prabha, Vijay
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4557255/
https://www.ncbi.nlm.nih.gov/pubmed/26261170
http://dx.doi.org/10.4103/0971-5916.162127
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author Bhandari, Praveen
Prabha, Vijay
author_facet Bhandari, Praveen
Prabha, Vijay
author_sort Bhandari, Praveen
collection PubMed
description BACKGROUND & OBJECTIVES: Urogenital infections of bacterial origin have a high incidence among the female population at reproductive age, affecting the fertility. Strains of Escherichia coli can colonize the vagina and replace natural microflora. Lactobacillus the predominant vaginal microorganism in healthy women, maintains the acidic vaginal pH which inhibits pathogenic microorganisms. Studies on Lactobacillus have shown that these can inhibit E. coli growth and vaginal colonization. An alternative therapeutic approach to antimicrobial therapy is to re-establish Lactobacillus in this microbiome through probiotic administration to resurge fertility. Therefore, the aim of the present study was to determine the capability of L. plantarum 2621 strain with probiotic properties, to prevent the vaginal colonization of E. coli causing agglutination of sperms and to evaluate its profertility effect in a murine model. METHODS: Screened mice were divided into five groups i.e. control group, E. coli group, Lactobacillus group, prophylactic and therapeutic groups. The control group was infused with 20 µl PBS, E.coli group was administered with 10(6) cfu/20 µl E. coli, and probiotic group was administered with Lactobacillus (10(8) cfu/20 µl) for 10 consecutive days. In prophylactic group, the vagina was colonized with 10 consecutive doses of Lactobacillus (10(8) cfu/20 µl). After 24 h, it was followed by 10 day intravaginal infection with E. coli (10(6) cfu/20 µl) whereas for the therapeutic group vagina was colonized with (10(6) cfu/20 µl) E. coli for 10 consecutive days, followed by 10 day intravaginal administration with Lactobacillus after 24 h. RESULTS: Upon mating and completion of gestation period, control, probiotic and the therapeutic groups had litters in contrast to the prophylactic group and the group administered with E. coli. INTERPRETATION & CONCLUSIONS: Results indicated that Lactobacillus intermitted colonization of pathogenic strains that resulted in reinforcement of natural microflora and resurge fertility.
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spelling pubmed-45572552015-09-21 Evaluation of profertility effect of probiotic Lactobacillus plantarum 2621 in a murine model Bhandari, Praveen Prabha, Vijay Indian J Med Res Original Article BACKGROUND & OBJECTIVES: Urogenital infections of bacterial origin have a high incidence among the female population at reproductive age, affecting the fertility. Strains of Escherichia coli can colonize the vagina and replace natural microflora. Lactobacillus the predominant vaginal microorganism in healthy women, maintains the acidic vaginal pH which inhibits pathogenic microorganisms. Studies on Lactobacillus have shown that these can inhibit E. coli growth and vaginal colonization. An alternative therapeutic approach to antimicrobial therapy is to re-establish Lactobacillus in this microbiome through probiotic administration to resurge fertility. Therefore, the aim of the present study was to determine the capability of L. plantarum 2621 strain with probiotic properties, to prevent the vaginal colonization of E. coli causing agglutination of sperms and to evaluate its profertility effect in a murine model. METHODS: Screened mice were divided into five groups i.e. control group, E. coli group, Lactobacillus group, prophylactic and therapeutic groups. The control group was infused with 20 µl PBS, E.coli group was administered with 10(6) cfu/20 µl E. coli, and probiotic group was administered with Lactobacillus (10(8) cfu/20 µl) for 10 consecutive days. In prophylactic group, the vagina was colonized with 10 consecutive doses of Lactobacillus (10(8) cfu/20 µl). After 24 h, it was followed by 10 day intravaginal infection with E. coli (10(6) cfu/20 µl) whereas for the therapeutic group vagina was colonized with (10(6) cfu/20 µl) E. coli for 10 consecutive days, followed by 10 day intravaginal administration with Lactobacillus after 24 h. RESULTS: Upon mating and completion of gestation period, control, probiotic and the therapeutic groups had litters in contrast to the prophylactic group and the group administered with E. coli. INTERPRETATION & CONCLUSIONS: Results indicated that Lactobacillus intermitted colonization of pathogenic strains that resulted in reinforcement of natural microflora and resurge fertility. Medknow Publications & Media Pvt Ltd 2015-07 /pmc/articles/PMC4557255/ /pubmed/26261170 http://dx.doi.org/10.4103/0971-5916.162127 Text en Copyright: © Indian Journal of Medical Research http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Bhandari, Praveen
Prabha, Vijay
Evaluation of profertility effect of probiotic Lactobacillus plantarum 2621 in a murine model
title Evaluation of profertility effect of probiotic Lactobacillus plantarum 2621 in a murine model
title_full Evaluation of profertility effect of probiotic Lactobacillus plantarum 2621 in a murine model
title_fullStr Evaluation of profertility effect of probiotic Lactobacillus plantarum 2621 in a murine model
title_full_unstemmed Evaluation of profertility effect of probiotic Lactobacillus plantarum 2621 in a murine model
title_short Evaluation of profertility effect of probiotic Lactobacillus plantarum 2621 in a murine model
title_sort evaluation of profertility effect of probiotic lactobacillus plantarum 2621 in a murine model
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4557255/
https://www.ncbi.nlm.nih.gov/pubmed/26261170
http://dx.doi.org/10.4103/0971-5916.162127
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