Developmental Decrease of Neuronal Chloride Concentration Is Independent of Trauma in Thalamocortical Brain Slices
The intraneuronal chloride concentration ([Cl(-)](i)) is paramount for determining the polarity of signaling at GABA(A) synapses in the central nervous system. Sectioning hippocampal brain slices increases [Cl(-)](i) in the superficial layers. It is not known whether cutting trauma also increases [C...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Public Library of Science
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4919081/ https://www.ncbi.nlm.nih.gov/pubmed/27337272 http://dx.doi.org/10.1371/journal.pone.0158012 |
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author | Glykys, Joseph Staley, Kevin J. |
author_facet | Glykys, Joseph Staley, Kevin J. |
author_sort | Glykys, Joseph |
collection | PubMed |
description | The intraneuronal chloride concentration ([Cl(-)](i)) is paramount for determining the polarity of signaling at GABA(A) synapses in the central nervous system. Sectioning hippocampal brain slices increases [Cl(-)](i) in the superficial layers. It is not known whether cutting trauma also increases [Cl(-)](i) in the neocortex and thalamus, and whether the effects of trauma change during development. We used Cl(-) imaging to study the [Cl(-)](i) vs. the distance from the cut surface in acute thalamocortical slices from mice at developmental ages ranging from post-natal day 5 (P5) to P20. We demonstrate: 1) [Cl(-)](i) is higher in the most superficial areas in both neocortical and thalamic brain slices at all ages tested and, 2) there is a developmental decrease in [Cl(-)](i) that is independent of acute trauma caused by brain slicing. We conclude that [Cl(-)](i) has a developmental progression during P5-20 in both the neocortex and thalamus. However, in both brain regions and during development the neurons closest to the slicing trauma have an elevated [Cl(-)](i). |
format | Online Article Text |
id | pubmed-4919081 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-49190812016-07-08 Developmental Decrease of Neuronal Chloride Concentration Is Independent of Trauma in Thalamocortical Brain Slices Glykys, Joseph Staley, Kevin J. PLoS One Research Article The intraneuronal chloride concentration ([Cl(-)](i)) is paramount for determining the polarity of signaling at GABA(A) synapses in the central nervous system. Sectioning hippocampal brain slices increases [Cl(-)](i) in the superficial layers. It is not known whether cutting trauma also increases [Cl(-)](i) in the neocortex and thalamus, and whether the effects of trauma change during development. We used Cl(-) imaging to study the [Cl(-)](i) vs. the distance from the cut surface in acute thalamocortical slices from mice at developmental ages ranging from post-natal day 5 (P5) to P20. We demonstrate: 1) [Cl(-)](i) is higher in the most superficial areas in both neocortical and thalamic brain slices at all ages tested and, 2) there is a developmental decrease in [Cl(-)](i) that is independent of acute trauma caused by brain slicing. We conclude that [Cl(-)](i) has a developmental progression during P5-20 in both the neocortex and thalamus. However, in both brain regions and during development the neurons closest to the slicing trauma have an elevated [Cl(-)](i). Public Library of Science 2016-06-23 /pmc/articles/PMC4919081/ /pubmed/27337272 http://dx.doi.org/10.1371/journal.pone.0158012 Text en © 2016 Glykys, Staley http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Glykys, Joseph Staley, Kevin J. Developmental Decrease of Neuronal Chloride Concentration Is Independent of Trauma in Thalamocortical Brain Slices |
title | Developmental Decrease of Neuronal Chloride Concentration Is Independent of Trauma in Thalamocortical Brain Slices |
title_full | Developmental Decrease of Neuronal Chloride Concentration Is Independent of Trauma in Thalamocortical Brain Slices |
title_fullStr | Developmental Decrease of Neuronal Chloride Concentration Is Independent of Trauma in Thalamocortical Brain Slices |
title_full_unstemmed | Developmental Decrease of Neuronal Chloride Concentration Is Independent of Trauma in Thalamocortical Brain Slices |
title_short | Developmental Decrease of Neuronal Chloride Concentration Is Independent of Trauma in Thalamocortical Brain Slices |
title_sort | developmental decrease of neuronal chloride concentration is independent of trauma in thalamocortical brain slices |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4919081/ https://www.ncbi.nlm.nih.gov/pubmed/27337272 http://dx.doi.org/10.1371/journal.pone.0158012 |
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