Developmental Decrease of Neuronal Chloride Concentration Is Independent of Trauma in Thalamocortical Brain Slices

The intraneuronal chloride concentration ([Cl(-)](i)) is paramount for determining the polarity of signaling at GABA(A) synapses in the central nervous system. Sectioning hippocampal brain slices increases [Cl(-)](i) in the superficial layers. It is not known whether cutting trauma also increases [C...

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Autores principales: Glykys, Joseph, Staley, Kevin J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4919081/
https://www.ncbi.nlm.nih.gov/pubmed/27337272
http://dx.doi.org/10.1371/journal.pone.0158012
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author Glykys, Joseph
Staley, Kevin J.
author_facet Glykys, Joseph
Staley, Kevin J.
author_sort Glykys, Joseph
collection PubMed
description The intraneuronal chloride concentration ([Cl(-)](i)) is paramount for determining the polarity of signaling at GABA(A) synapses in the central nervous system. Sectioning hippocampal brain slices increases [Cl(-)](i) in the superficial layers. It is not known whether cutting trauma also increases [Cl(-)](i) in the neocortex and thalamus, and whether the effects of trauma change during development. We used Cl(-) imaging to study the [Cl(-)](i) vs. the distance from the cut surface in acute thalamocortical slices from mice at developmental ages ranging from post-natal day 5 (P5) to P20. We demonstrate: 1) [Cl(-)](i) is higher in the most superficial areas in both neocortical and thalamic brain slices at all ages tested and, 2) there is a developmental decrease in [Cl(-)](i) that is independent of acute trauma caused by brain slicing. We conclude that [Cl(-)](i) has a developmental progression during P5-20 in both the neocortex and thalamus. However, in both brain regions and during development the neurons closest to the slicing trauma have an elevated [Cl(-)](i).
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spelling pubmed-49190812016-07-08 Developmental Decrease of Neuronal Chloride Concentration Is Independent of Trauma in Thalamocortical Brain Slices Glykys, Joseph Staley, Kevin J. PLoS One Research Article The intraneuronal chloride concentration ([Cl(-)](i)) is paramount for determining the polarity of signaling at GABA(A) synapses in the central nervous system. Sectioning hippocampal brain slices increases [Cl(-)](i) in the superficial layers. It is not known whether cutting trauma also increases [Cl(-)](i) in the neocortex and thalamus, and whether the effects of trauma change during development. We used Cl(-) imaging to study the [Cl(-)](i) vs. the distance from the cut surface in acute thalamocortical slices from mice at developmental ages ranging from post-natal day 5 (P5) to P20. We demonstrate: 1) [Cl(-)](i) is higher in the most superficial areas in both neocortical and thalamic brain slices at all ages tested and, 2) there is a developmental decrease in [Cl(-)](i) that is independent of acute trauma caused by brain slicing. We conclude that [Cl(-)](i) has a developmental progression during P5-20 in both the neocortex and thalamus. However, in both brain regions and during development the neurons closest to the slicing trauma have an elevated [Cl(-)](i). Public Library of Science 2016-06-23 /pmc/articles/PMC4919081/ /pubmed/27337272 http://dx.doi.org/10.1371/journal.pone.0158012 Text en © 2016 Glykys, Staley http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Glykys, Joseph
Staley, Kevin J.
Developmental Decrease of Neuronal Chloride Concentration Is Independent of Trauma in Thalamocortical Brain Slices
title Developmental Decrease of Neuronal Chloride Concentration Is Independent of Trauma in Thalamocortical Brain Slices
title_full Developmental Decrease of Neuronal Chloride Concentration Is Independent of Trauma in Thalamocortical Brain Slices
title_fullStr Developmental Decrease of Neuronal Chloride Concentration Is Independent of Trauma in Thalamocortical Brain Slices
title_full_unstemmed Developmental Decrease of Neuronal Chloride Concentration Is Independent of Trauma in Thalamocortical Brain Slices
title_short Developmental Decrease of Neuronal Chloride Concentration Is Independent of Trauma in Thalamocortical Brain Slices
title_sort developmental decrease of neuronal chloride concentration is independent of trauma in thalamocortical brain slices
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4919081/
https://www.ncbi.nlm.nih.gov/pubmed/27337272
http://dx.doi.org/10.1371/journal.pone.0158012
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