Cytoprotective Mechanism of Cyanidin and Delphinidin against Oxidative Stress-Induced Tenofibroblast Death
Age-related rotator cuff tendon degeneration is related to tenofibroblast apoptosis. Anthocyanins reduce oxidative stress-induced apoptotic cell death in tenofibroblasts. The current study investigated the presence of cell protective effects in cyanidin and delphinidin, the most common aglycon forms...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Korean Society of Applied Pharmacology
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4930287/ https://www.ncbi.nlm.nih.gov/pubmed/27098861 http://dx.doi.org/10.4062/biomolther.2015.169 |
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author | Nam, Dae Cheol Hah, Young Sool Nam, Jung Been Kim, Ra Jeong Park, Hyung Bin |
author_facet | Nam, Dae Cheol Hah, Young Sool Nam, Jung Been Kim, Ra Jeong Park, Hyung Bin |
author_sort | Nam, Dae Cheol |
collection | PubMed |
description | Age-related rotator cuff tendon degeneration is related to tenofibroblast apoptosis. Anthocyanins reduce oxidative stress-induced apoptotic cell death in tenofibroblasts. The current study investigated the presence of cell protective effects in cyanidin and delphinidin, the most common aglycon forms of anthocyanins. We determined whether these anthocyanidins have antiapoptotic and antinecrotic effects in tenofibroblasts exposed to H(2)O(2), and evaluated their biomolecular mechanisms. Both cyanidin and delphinidin inhibited H(2)O(2)-induced apoptosis in a dose-dependent manner. However, at concentrations of 100 μg/ml or greater, delphinidin showed cytotoxicity against tenofibroblasts and a decreased antinecrotic effect. Cyanidin and delphinidin both showed inhibitory effects on the H(2)O(2)-induced increase in intracellular ROS formation and the activation of ERK1/2 and JNK. In conclusion, both cyanidin and delphinidin have cytoprotective effects on cultured tenofibroblasts exposed to H(2)O(2). These results suggest that cyanidin and delphinidin are both beneficial for the treatment of oxidative stress-mediated tenofibroblast cell death, but their working concentrations are different. |
format | Online Article Text |
id | pubmed-4930287 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | The Korean Society of Applied Pharmacology |
record_format | MEDLINE/PubMed |
spelling | pubmed-49302872016-07-15 Cytoprotective Mechanism of Cyanidin and Delphinidin against Oxidative Stress-Induced Tenofibroblast Death Nam, Dae Cheol Hah, Young Sool Nam, Jung Been Kim, Ra Jeong Park, Hyung Bin Biomol Ther (Seoul) Original Article Age-related rotator cuff tendon degeneration is related to tenofibroblast apoptosis. Anthocyanins reduce oxidative stress-induced apoptotic cell death in tenofibroblasts. The current study investigated the presence of cell protective effects in cyanidin and delphinidin, the most common aglycon forms of anthocyanins. We determined whether these anthocyanidins have antiapoptotic and antinecrotic effects in tenofibroblasts exposed to H(2)O(2), and evaluated their biomolecular mechanisms. Both cyanidin and delphinidin inhibited H(2)O(2)-induced apoptosis in a dose-dependent manner. However, at concentrations of 100 μg/ml or greater, delphinidin showed cytotoxicity against tenofibroblasts and a decreased antinecrotic effect. Cyanidin and delphinidin both showed inhibitory effects on the H(2)O(2)-induced increase in intracellular ROS formation and the activation of ERK1/2 and JNK. In conclusion, both cyanidin and delphinidin have cytoprotective effects on cultured tenofibroblasts exposed to H(2)O(2). These results suggest that cyanidin and delphinidin are both beneficial for the treatment of oxidative stress-mediated tenofibroblast cell death, but their working concentrations are different. The Korean Society of Applied Pharmacology 2016-07 2016-07-01 /pmc/articles/PMC4930287/ /pubmed/27098861 http://dx.doi.org/10.4062/biomolther.2015.169 Text en Copyright ©2016, The Korean Society of Applied Pharmacology http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Nam, Dae Cheol Hah, Young Sool Nam, Jung Been Kim, Ra Jeong Park, Hyung Bin Cytoprotective Mechanism of Cyanidin and Delphinidin against Oxidative Stress-Induced Tenofibroblast Death |
title | Cytoprotective Mechanism of Cyanidin and Delphinidin against Oxidative Stress-Induced Tenofibroblast Death |
title_full | Cytoprotective Mechanism of Cyanidin and Delphinidin against Oxidative Stress-Induced Tenofibroblast Death |
title_fullStr | Cytoprotective Mechanism of Cyanidin and Delphinidin against Oxidative Stress-Induced Tenofibroblast Death |
title_full_unstemmed | Cytoprotective Mechanism of Cyanidin and Delphinidin against Oxidative Stress-Induced Tenofibroblast Death |
title_short | Cytoprotective Mechanism of Cyanidin and Delphinidin against Oxidative Stress-Induced Tenofibroblast Death |
title_sort | cytoprotective mechanism of cyanidin and delphinidin against oxidative stress-induced tenofibroblast death |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4930287/ https://www.ncbi.nlm.nih.gov/pubmed/27098861 http://dx.doi.org/10.4062/biomolther.2015.169 |
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