Cytoprotective Mechanism of Cyanidin and Delphinidin against Oxidative Stress-Induced Tenofibroblast Death

Age-related rotator cuff tendon degeneration is related to tenofibroblast apoptosis. Anthocyanins reduce oxidative stress-induced apoptotic cell death in tenofibroblasts. The current study investigated the presence of cell protective effects in cyanidin and delphinidin, the most common aglycon forms...

Descripción completa

Detalles Bibliográficos
Autores principales: Nam, Dae Cheol, Hah, Young Sool, Nam, Jung Been, Kim, Ra Jeong, Park, Hyung Bin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Society of Applied Pharmacology 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4930287/
https://www.ncbi.nlm.nih.gov/pubmed/27098861
http://dx.doi.org/10.4062/biomolther.2015.169
_version_ 1782440730001670144
author Nam, Dae Cheol
Hah, Young Sool
Nam, Jung Been
Kim, Ra Jeong
Park, Hyung Bin
author_facet Nam, Dae Cheol
Hah, Young Sool
Nam, Jung Been
Kim, Ra Jeong
Park, Hyung Bin
author_sort Nam, Dae Cheol
collection PubMed
description Age-related rotator cuff tendon degeneration is related to tenofibroblast apoptosis. Anthocyanins reduce oxidative stress-induced apoptotic cell death in tenofibroblasts. The current study investigated the presence of cell protective effects in cyanidin and delphinidin, the most common aglycon forms of anthocyanins. We determined whether these anthocyanidins have antiapoptotic and antinecrotic effects in tenofibroblasts exposed to H(2)O(2), and evaluated their biomolecular mechanisms. Both cyanidin and delphinidin inhibited H(2)O(2)-induced apoptosis in a dose-dependent manner. However, at concentrations of 100 μg/ml or greater, delphinidin showed cytotoxicity against tenofibroblasts and a decreased antinecrotic effect. Cyanidin and delphinidin both showed inhibitory effects on the H(2)O(2)-induced increase in intracellular ROS formation and the activation of ERK1/2 and JNK. In conclusion, both cyanidin and delphinidin have cytoprotective effects on cultured tenofibroblasts exposed to H(2)O(2). These results suggest that cyanidin and delphinidin are both beneficial for the treatment of oxidative stress-mediated tenofibroblast cell death, but their working concentrations are different.
format Online
Article
Text
id pubmed-4930287
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher The Korean Society of Applied Pharmacology
record_format MEDLINE/PubMed
spelling pubmed-49302872016-07-15 Cytoprotective Mechanism of Cyanidin and Delphinidin against Oxidative Stress-Induced Tenofibroblast Death Nam, Dae Cheol Hah, Young Sool Nam, Jung Been Kim, Ra Jeong Park, Hyung Bin Biomol Ther (Seoul) Original Article Age-related rotator cuff tendon degeneration is related to tenofibroblast apoptosis. Anthocyanins reduce oxidative stress-induced apoptotic cell death in tenofibroblasts. The current study investigated the presence of cell protective effects in cyanidin and delphinidin, the most common aglycon forms of anthocyanins. We determined whether these anthocyanidins have antiapoptotic and antinecrotic effects in tenofibroblasts exposed to H(2)O(2), and evaluated their biomolecular mechanisms. Both cyanidin and delphinidin inhibited H(2)O(2)-induced apoptosis in a dose-dependent manner. However, at concentrations of 100 μg/ml or greater, delphinidin showed cytotoxicity against tenofibroblasts and a decreased antinecrotic effect. Cyanidin and delphinidin both showed inhibitory effects on the H(2)O(2)-induced increase in intracellular ROS formation and the activation of ERK1/2 and JNK. In conclusion, both cyanidin and delphinidin have cytoprotective effects on cultured tenofibroblasts exposed to H(2)O(2). These results suggest that cyanidin and delphinidin are both beneficial for the treatment of oxidative stress-mediated tenofibroblast cell death, but their working concentrations are different. The Korean Society of Applied Pharmacology 2016-07 2016-07-01 /pmc/articles/PMC4930287/ /pubmed/27098861 http://dx.doi.org/10.4062/biomolther.2015.169 Text en Copyright ©2016, The Korean Society of Applied Pharmacology http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Nam, Dae Cheol
Hah, Young Sool
Nam, Jung Been
Kim, Ra Jeong
Park, Hyung Bin
Cytoprotective Mechanism of Cyanidin and Delphinidin against Oxidative Stress-Induced Tenofibroblast Death
title Cytoprotective Mechanism of Cyanidin and Delphinidin against Oxidative Stress-Induced Tenofibroblast Death
title_full Cytoprotective Mechanism of Cyanidin and Delphinidin against Oxidative Stress-Induced Tenofibroblast Death
title_fullStr Cytoprotective Mechanism of Cyanidin and Delphinidin against Oxidative Stress-Induced Tenofibroblast Death
title_full_unstemmed Cytoprotective Mechanism of Cyanidin and Delphinidin against Oxidative Stress-Induced Tenofibroblast Death
title_short Cytoprotective Mechanism of Cyanidin and Delphinidin against Oxidative Stress-Induced Tenofibroblast Death
title_sort cytoprotective mechanism of cyanidin and delphinidin against oxidative stress-induced tenofibroblast death
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4930287/
https://www.ncbi.nlm.nih.gov/pubmed/27098861
http://dx.doi.org/10.4062/biomolther.2015.169
work_keys_str_mv AT namdaecheol cytoprotectivemechanismofcyanidinanddelphinidinagainstoxidativestressinducedtenofibroblastdeath
AT hahyoungsool cytoprotectivemechanismofcyanidinanddelphinidinagainstoxidativestressinducedtenofibroblastdeath
AT namjungbeen cytoprotectivemechanismofcyanidinanddelphinidinagainstoxidativestressinducedtenofibroblastdeath
AT kimrajeong cytoprotectivemechanismofcyanidinanddelphinidinagainstoxidativestressinducedtenofibroblastdeath
AT parkhyungbin cytoprotectivemechanismofcyanidinanddelphinidinagainstoxidativestressinducedtenofibroblastdeath