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PFA fixation enables artifact-free super-resolution imaging of the actin cytoskeleton and associated proteins

Super-resolution microscopy (SRM) allows precise localization of proteins in cellular organelles and structures, including the actin cytoskeleton. Yet sample preparation protocols for SRM are rather anecdotal and still being optimized. Thus, SRM-based imaging of the actin cytoskeleton and associated...

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Detalles Bibliográficos
Autores principales: Leyton-Puig, Daniela, Kedziora, Katarzyna M., Isogai, Tadamoto, van den Broek, Bram, Jalink, Kees, Innocenti, Metello
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Company of Biologists Ltd 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4958280/
https://www.ncbi.nlm.nih.gov/pubmed/27378434
http://dx.doi.org/10.1242/bio.019570