1α,25(OH)(2)D(3) Suppresses the Migration of Ovarian Cancer SKOV-3 Cells through the Inhibition of Epithelial–Mesenchymal Transition

Ovarian cancer is the most lethal gynecological malignancy due to its high metastatic ability. Epithelial-mesenchymal transition (EMT) is essential during both follicular rupture and epithelium regeneration. However, it may also accelerate the progression of ovarian carcinomas. Experimental studies...

Descripción completa

Detalles Bibliográficos
Autores principales: Hou, Yong-Feng, Gao, Si-Hai, Wang, Ping, Zhang, He-Mei, Liu, Li-Zhi, Ye, Meng-Xuan, Zhou, Guang-Ming, Zhang, Zeng-Li, Li, Bing-Yan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5000682/
https://www.ncbi.nlm.nih.gov/pubmed/27548154
http://dx.doi.org/10.3390/ijms17081285
_version_ 1782450337993457664
author Hou, Yong-Feng
Gao, Si-Hai
Wang, Ping
Zhang, He-Mei
Liu, Li-Zhi
Ye, Meng-Xuan
Zhou, Guang-Ming
Zhang, Zeng-Li
Li, Bing-Yan
author_facet Hou, Yong-Feng
Gao, Si-Hai
Wang, Ping
Zhang, He-Mei
Liu, Li-Zhi
Ye, Meng-Xuan
Zhou, Guang-Ming
Zhang, Zeng-Li
Li, Bing-Yan
author_sort Hou, Yong-Feng
collection PubMed
description Ovarian cancer is the most lethal gynecological malignancy due to its high metastatic ability. Epithelial-mesenchymal transition (EMT) is essential during both follicular rupture and epithelium regeneration. However, it may also accelerate the progression of ovarian carcinomas. Experimental studies have found that 1α,25-dihydroxyvitamin-D3 [1α,25(OH)(2)D(3)] can inhibit the proliferation of ovarian cancer cells. In this study, we investigated whether 1α,25(OH)(2)D(3) could inhibit the migration of ovarian cancer cells via regulating EMT. We established a model of transient transforming growth factor-β1(TGF-β1)-induced EMT in human ovarian adenocarcinoma cell line SKOV-3 cells. Results showed that, compared with control, 1α,25(OH)(2)D(3) not only inhibited the migration and the invasion of SKOV-3 cells, but also promoted the acquisition of an epithelial phenotype of SKOV-3 cells treated with TGF-β1. We discovered that 1α,25(OH)(2)D(3) increased the expression of epithelial marker E-cadherin and decreased the level of mesenchymal marker, Vimentin, which was associated with the elevated expression of VDR. Moreover, 1α,25(OH)(2)D(3) reduced the expression level of transcription factors of EMT, such as slug, snail, and β-catenin. These results indicate that 1α,25(OH)(2)D(3) suppresses the migration and invasion of ovarian cancer cells by inhibiting EMT, implying that 1α,25(OH)(2)D(3) might be a potential therapeutic agent for the treatment of ovarian cancer.
format Online
Article
Text
id pubmed-5000682
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-50006822016-09-01 1α,25(OH)(2)D(3) Suppresses the Migration of Ovarian Cancer SKOV-3 Cells through the Inhibition of Epithelial–Mesenchymal Transition Hou, Yong-Feng Gao, Si-Hai Wang, Ping Zhang, He-Mei Liu, Li-Zhi Ye, Meng-Xuan Zhou, Guang-Ming Zhang, Zeng-Li Li, Bing-Yan Int J Mol Sci Article Ovarian cancer is the most lethal gynecological malignancy due to its high metastatic ability. Epithelial-mesenchymal transition (EMT) is essential during both follicular rupture and epithelium regeneration. However, it may also accelerate the progression of ovarian carcinomas. Experimental studies have found that 1α,25-dihydroxyvitamin-D3 [1α,25(OH)(2)D(3)] can inhibit the proliferation of ovarian cancer cells. In this study, we investigated whether 1α,25(OH)(2)D(3) could inhibit the migration of ovarian cancer cells via regulating EMT. We established a model of transient transforming growth factor-β1(TGF-β1)-induced EMT in human ovarian adenocarcinoma cell line SKOV-3 cells. Results showed that, compared with control, 1α,25(OH)(2)D(3) not only inhibited the migration and the invasion of SKOV-3 cells, but also promoted the acquisition of an epithelial phenotype of SKOV-3 cells treated with TGF-β1. We discovered that 1α,25(OH)(2)D(3) increased the expression of epithelial marker E-cadherin and decreased the level of mesenchymal marker, Vimentin, which was associated with the elevated expression of VDR. Moreover, 1α,25(OH)(2)D(3) reduced the expression level of transcription factors of EMT, such as slug, snail, and β-catenin. These results indicate that 1α,25(OH)(2)D(3) suppresses the migration and invasion of ovarian cancer cells by inhibiting EMT, implying that 1α,25(OH)(2)D(3) might be a potential therapeutic agent for the treatment of ovarian cancer. MDPI 2016-08-19 /pmc/articles/PMC5000682/ /pubmed/27548154 http://dx.doi.org/10.3390/ijms17081285 Text en © 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Hou, Yong-Feng
Gao, Si-Hai
Wang, Ping
Zhang, He-Mei
Liu, Li-Zhi
Ye, Meng-Xuan
Zhou, Guang-Ming
Zhang, Zeng-Li
Li, Bing-Yan
1α,25(OH)(2)D(3) Suppresses the Migration of Ovarian Cancer SKOV-3 Cells through the Inhibition of Epithelial–Mesenchymal Transition
title 1α,25(OH)(2)D(3) Suppresses the Migration of Ovarian Cancer SKOV-3 Cells through the Inhibition of Epithelial–Mesenchymal Transition
title_full 1α,25(OH)(2)D(3) Suppresses the Migration of Ovarian Cancer SKOV-3 Cells through the Inhibition of Epithelial–Mesenchymal Transition
title_fullStr 1α,25(OH)(2)D(3) Suppresses the Migration of Ovarian Cancer SKOV-3 Cells through the Inhibition of Epithelial–Mesenchymal Transition
title_full_unstemmed 1α,25(OH)(2)D(3) Suppresses the Migration of Ovarian Cancer SKOV-3 Cells through the Inhibition of Epithelial–Mesenchymal Transition
title_short 1α,25(OH)(2)D(3) Suppresses the Migration of Ovarian Cancer SKOV-3 Cells through the Inhibition of Epithelial–Mesenchymal Transition
title_sort 1α,25(oh)(2)d(3) suppresses the migration of ovarian cancer skov-3 cells through the inhibition of epithelial–mesenchymal transition
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5000682/
https://www.ncbi.nlm.nih.gov/pubmed/27548154
http://dx.doi.org/10.3390/ijms17081285
work_keys_str_mv AT houyongfeng 1a25oh2d3suppressesthemigrationofovariancancerskov3cellsthroughtheinhibitionofepithelialmesenchymaltransition
AT gaosihai 1a25oh2d3suppressesthemigrationofovariancancerskov3cellsthroughtheinhibitionofepithelialmesenchymaltransition
AT wangping 1a25oh2d3suppressesthemigrationofovariancancerskov3cellsthroughtheinhibitionofepithelialmesenchymaltransition
AT zhanghemei 1a25oh2d3suppressesthemigrationofovariancancerskov3cellsthroughtheinhibitionofepithelialmesenchymaltransition
AT liulizhi 1a25oh2d3suppressesthemigrationofovariancancerskov3cellsthroughtheinhibitionofepithelialmesenchymaltransition
AT yemengxuan 1a25oh2d3suppressesthemigrationofovariancancerskov3cellsthroughtheinhibitionofepithelialmesenchymaltransition
AT zhouguangming 1a25oh2d3suppressesthemigrationofovariancancerskov3cellsthroughtheinhibitionofepithelialmesenchymaltransition
AT zhangzengli 1a25oh2d3suppressesthemigrationofovariancancerskov3cellsthroughtheinhibitionofepithelialmesenchymaltransition
AT libingyan 1a25oh2d3suppressesthemigrationofovariancancerskov3cellsthroughtheinhibitionofepithelialmesenchymaltransition