The effect of acute and chronic sprint‐interval training on LRP130, SIRT3, and PGC‐1α expression in human skeletal muscle
This study examined changes in LRP130 gene and protein expression in response to an acute bout of sprint‐interval training (SIT) and 6 weeks of SIT in human skeletal muscle. In addition, we investigated the relationships between changes in LRP130, SIRT3, and PGC‐1α gene or protein expression. Fourte...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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John Wiley and Sons Inc.
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5027339/ https://www.ncbi.nlm.nih.gov/pubmed/27604398 http://dx.doi.org/10.14814/phy2.12879 |
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author | Edgett, Brittany A. Bonafiglia, Jacob T. Baechler, Brittany L. Quadrilatero, Joe Gurd, Brendon J. |
author_facet | Edgett, Brittany A. Bonafiglia, Jacob T. Baechler, Brittany L. Quadrilatero, Joe Gurd, Brendon J. |
author_sort | Edgett, Brittany A. |
collection | PubMed |
description | This study examined changes in LRP130 gene and protein expression in response to an acute bout of sprint‐interval training (SIT) and 6 weeks of SIT in human skeletal muscle. In addition, we investigated the relationships between changes in LRP130, SIRT3, and PGC‐1α gene or protein expression. Fourteen recreationally active men (age: 22.0 ± 2.4 years) performed a single bout of SIT (eight, 20‐sec intervals at ~170% of VO (2)peak work rate, separated by 10 sec of rest). Muscle biopsies were obtained at rest (PRE) and 3 h post‐exercise. The same participants then underwent a 6 week SIT program with biopsies after 2 (MID) and 6 (POST) weeks of training. In response to an acute bout of SIT, PGC‐1α mRNA expression increased (284%, P < 0.001); however, LRP130 and SIRT3 remained unchanged. VO (2)peak and fiber‐specific SDH activity increased in response to training (P < 0.01). LRP130, SIRT3, and PGC‐1α protein expression were also unaltered following 2 and 6 weeks of SIT. There were no significant correlations between LRP130, SIRT3, or PGC‐1α mRNA expression in response to acute SIT. However, changes in protein expression of LRP130, SIRT3, and PGC‐1α were positively correlated at several time points with large effect sizes, which suggest that the regulation of these proteins may be coordinated in human skeletal muscle. Future studies should investigate other exercise protocols known to increase PGC‐1α and SIRT3 protein, like longer duration steady‐state exercise, to identify if LRP130 expression can be altered in response to exercise. |
format | Online Article Text |
id | pubmed-5027339 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-50273392017-03-07 The effect of acute and chronic sprint‐interval training on LRP130, SIRT3, and PGC‐1α expression in human skeletal muscle Edgett, Brittany A. Bonafiglia, Jacob T. Baechler, Brittany L. Quadrilatero, Joe Gurd, Brendon J. Physiol Rep Original Research This study examined changes in LRP130 gene and protein expression in response to an acute bout of sprint‐interval training (SIT) and 6 weeks of SIT in human skeletal muscle. In addition, we investigated the relationships between changes in LRP130, SIRT3, and PGC‐1α gene or protein expression. Fourteen recreationally active men (age: 22.0 ± 2.4 years) performed a single bout of SIT (eight, 20‐sec intervals at ~170% of VO (2)peak work rate, separated by 10 sec of rest). Muscle biopsies were obtained at rest (PRE) and 3 h post‐exercise. The same participants then underwent a 6 week SIT program with biopsies after 2 (MID) and 6 (POST) weeks of training. In response to an acute bout of SIT, PGC‐1α mRNA expression increased (284%, P < 0.001); however, LRP130 and SIRT3 remained unchanged. VO (2)peak and fiber‐specific SDH activity increased in response to training (P < 0.01). LRP130, SIRT3, and PGC‐1α protein expression were also unaltered following 2 and 6 weeks of SIT. There were no significant correlations between LRP130, SIRT3, or PGC‐1α mRNA expression in response to acute SIT. However, changes in protein expression of LRP130, SIRT3, and PGC‐1α were positively correlated at several time points with large effect sizes, which suggest that the regulation of these proteins may be coordinated in human skeletal muscle. Future studies should investigate other exercise protocols known to increase PGC‐1α and SIRT3 protein, like longer duration steady‐state exercise, to identify if LRP130 expression can be altered in response to exercise. John Wiley and Sons Inc. 2016-09-07 /pmc/articles/PMC5027339/ /pubmed/27604398 http://dx.doi.org/10.14814/phy2.12879 Text en © 2016 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of the American Physiological Society and The Physiological Society. This is an open access article under the terms of the Creative Commons Attribution (http://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Research Edgett, Brittany A. Bonafiglia, Jacob T. Baechler, Brittany L. Quadrilatero, Joe Gurd, Brendon J. The effect of acute and chronic sprint‐interval training on LRP130, SIRT3, and PGC‐1α expression in human skeletal muscle |
title | The effect of acute and chronic sprint‐interval training on LRP130, SIRT3, and PGC‐1α expression in human skeletal muscle |
title_full | The effect of acute and chronic sprint‐interval training on LRP130, SIRT3, and PGC‐1α expression in human skeletal muscle |
title_fullStr | The effect of acute and chronic sprint‐interval training on LRP130, SIRT3, and PGC‐1α expression in human skeletal muscle |
title_full_unstemmed | The effect of acute and chronic sprint‐interval training on LRP130, SIRT3, and PGC‐1α expression in human skeletal muscle |
title_short | The effect of acute and chronic sprint‐interval training on LRP130, SIRT3, and PGC‐1α expression in human skeletal muscle |
title_sort | effect of acute and chronic sprint‐interval training on lrp130, sirt3, and pgc‐1α expression in human skeletal muscle |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5027339/ https://www.ncbi.nlm.nih.gov/pubmed/27604398 http://dx.doi.org/10.14814/phy2.12879 |
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