The effect of acute and chronic sprint‐interval training on LRP130, SIRT3, and PGC‐1α expression in human skeletal muscle

This study examined changes in LRP130 gene and protein expression in response to an acute bout of sprint‐interval training (SIT) and 6 weeks of SIT in human skeletal muscle. In addition, we investigated the relationships between changes in LRP130, SIRT3, and PGC‐1α gene or protein expression. Fourte...

Descripción completa

Detalles Bibliográficos
Autores principales: Edgett, Brittany A., Bonafiglia, Jacob T., Baechler, Brittany L., Quadrilatero, Joe, Gurd, Brendon J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5027339/
https://www.ncbi.nlm.nih.gov/pubmed/27604398
http://dx.doi.org/10.14814/phy2.12879
_version_ 1782454226585124864
author Edgett, Brittany A.
Bonafiglia, Jacob T.
Baechler, Brittany L.
Quadrilatero, Joe
Gurd, Brendon J.
author_facet Edgett, Brittany A.
Bonafiglia, Jacob T.
Baechler, Brittany L.
Quadrilatero, Joe
Gurd, Brendon J.
author_sort Edgett, Brittany A.
collection PubMed
description This study examined changes in LRP130 gene and protein expression in response to an acute bout of sprint‐interval training (SIT) and 6 weeks of SIT in human skeletal muscle. In addition, we investigated the relationships between changes in LRP130, SIRT3, and PGC‐1α gene or protein expression. Fourteen recreationally active men (age: 22.0 ± 2.4 years) performed a single bout of SIT (eight, 20‐sec intervals at ~170% of VO (2)peak work rate, separated by 10 sec of rest). Muscle biopsies were obtained at rest (PRE) and 3 h post‐exercise. The same participants then underwent a 6 week SIT program with biopsies after 2 (MID) and 6 (POST) weeks of training. In response to an acute bout of SIT, PGC‐1α mRNA expression increased (284%, P < 0.001); however, LRP130 and SIRT3 remained unchanged. VO (2)peak and fiber‐specific SDH activity increased in response to training (P < 0.01). LRP130, SIRT3, and PGC‐1α protein expression were also unaltered following 2 and 6 weeks of SIT. There were no significant correlations between LRP130, SIRT3, or PGC‐1α mRNA expression in response to acute SIT. However, changes in protein expression of LRP130, SIRT3, and PGC‐1α were positively correlated at several time points with large effect sizes, which suggest that the regulation of these proteins may be coordinated in human skeletal muscle. Future studies should investigate other exercise protocols known to increase PGC‐1α and SIRT3 protein, like longer duration steady‐state exercise, to identify if LRP130 expression can be altered in response to exercise.
format Online
Article
Text
id pubmed-5027339
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher John Wiley and Sons Inc.
record_format MEDLINE/PubMed
spelling pubmed-50273392017-03-07 The effect of acute and chronic sprint‐interval training on LRP130, SIRT3, and PGC‐1α expression in human skeletal muscle Edgett, Brittany A. Bonafiglia, Jacob T. Baechler, Brittany L. Quadrilatero, Joe Gurd, Brendon J. Physiol Rep Original Research This study examined changes in LRP130 gene and protein expression in response to an acute bout of sprint‐interval training (SIT) and 6 weeks of SIT in human skeletal muscle. In addition, we investigated the relationships between changes in LRP130, SIRT3, and PGC‐1α gene or protein expression. Fourteen recreationally active men (age: 22.0 ± 2.4 years) performed a single bout of SIT (eight, 20‐sec intervals at ~170% of VO (2)peak work rate, separated by 10 sec of rest). Muscle biopsies were obtained at rest (PRE) and 3 h post‐exercise. The same participants then underwent a 6 week SIT program with biopsies after 2 (MID) and 6 (POST) weeks of training. In response to an acute bout of SIT, PGC‐1α mRNA expression increased (284%, P < 0.001); however, LRP130 and SIRT3 remained unchanged. VO (2)peak and fiber‐specific SDH activity increased in response to training (P < 0.01). LRP130, SIRT3, and PGC‐1α protein expression were also unaltered following 2 and 6 weeks of SIT. There were no significant correlations between LRP130, SIRT3, or PGC‐1α mRNA expression in response to acute SIT. However, changes in protein expression of LRP130, SIRT3, and PGC‐1α were positively correlated at several time points with large effect sizes, which suggest that the regulation of these proteins may be coordinated in human skeletal muscle. Future studies should investigate other exercise protocols known to increase PGC‐1α and SIRT3 protein, like longer duration steady‐state exercise, to identify if LRP130 expression can be altered in response to exercise. John Wiley and Sons Inc. 2016-09-07 /pmc/articles/PMC5027339/ /pubmed/27604398 http://dx.doi.org/10.14814/phy2.12879 Text en © 2016 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of the American Physiological Society and The Physiological Society. This is an open access article under the terms of the Creative Commons Attribution (http://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Research
Edgett, Brittany A.
Bonafiglia, Jacob T.
Baechler, Brittany L.
Quadrilatero, Joe
Gurd, Brendon J.
The effect of acute and chronic sprint‐interval training on LRP130, SIRT3, and PGC‐1α expression in human skeletal muscle
title The effect of acute and chronic sprint‐interval training on LRP130, SIRT3, and PGC‐1α expression in human skeletal muscle
title_full The effect of acute and chronic sprint‐interval training on LRP130, SIRT3, and PGC‐1α expression in human skeletal muscle
title_fullStr The effect of acute and chronic sprint‐interval training on LRP130, SIRT3, and PGC‐1α expression in human skeletal muscle
title_full_unstemmed The effect of acute and chronic sprint‐interval training on LRP130, SIRT3, and PGC‐1α expression in human skeletal muscle
title_short The effect of acute and chronic sprint‐interval training on LRP130, SIRT3, and PGC‐1α expression in human skeletal muscle
title_sort effect of acute and chronic sprint‐interval training on lrp130, sirt3, and pgc‐1α expression in human skeletal muscle
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5027339/
https://www.ncbi.nlm.nih.gov/pubmed/27604398
http://dx.doi.org/10.14814/phy2.12879
work_keys_str_mv AT edgettbrittanya theeffectofacuteandchronicsprintintervaltrainingonlrp130sirt3andpgc1aexpressioninhumanskeletalmuscle
AT bonafigliajacobt theeffectofacuteandchronicsprintintervaltrainingonlrp130sirt3andpgc1aexpressioninhumanskeletalmuscle
AT baechlerbrittanyl theeffectofacuteandchronicsprintintervaltrainingonlrp130sirt3andpgc1aexpressioninhumanskeletalmuscle
AT quadrilaterojoe theeffectofacuteandchronicsprintintervaltrainingonlrp130sirt3andpgc1aexpressioninhumanskeletalmuscle
AT gurdbrendonj theeffectofacuteandchronicsprintintervaltrainingonlrp130sirt3andpgc1aexpressioninhumanskeletalmuscle
AT edgettbrittanya effectofacuteandchronicsprintintervaltrainingonlrp130sirt3andpgc1aexpressioninhumanskeletalmuscle
AT bonafigliajacobt effectofacuteandchronicsprintintervaltrainingonlrp130sirt3andpgc1aexpressioninhumanskeletalmuscle
AT baechlerbrittanyl effectofacuteandchronicsprintintervaltrainingonlrp130sirt3andpgc1aexpressioninhumanskeletalmuscle
AT quadrilaterojoe effectofacuteandchronicsprintintervaltrainingonlrp130sirt3andpgc1aexpressioninhumanskeletalmuscle
AT gurdbrendonj effectofacuteandchronicsprintintervaltrainingonlrp130sirt3andpgc1aexpressioninhumanskeletalmuscle