The many faces of Pluripotency: in vitro adaptations of a continuum of in vivo states
Pluripotency defines the propensity of a cell to differentiate into, and generate, all somatic, as well as germ cells. The epiblast of the early mammalian embryo is the founder population of all germ layer derivatives and thus represents the bona fide in vivo pluripotent cell population. The so-call...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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BioMed Central
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5470286/ https://www.ncbi.nlm.nih.gov/pubmed/28610558 http://dx.doi.org/10.1186/s12861-017-0150-4 |
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author | Morgani, Sophie Nichols, Jennifer Hadjantonakis, Anna-Katerina |
author_facet | Morgani, Sophie Nichols, Jennifer Hadjantonakis, Anna-Katerina |
author_sort | Morgani, Sophie |
collection | PubMed |
description | Pluripotency defines the propensity of a cell to differentiate into, and generate, all somatic, as well as germ cells. The epiblast of the early mammalian embryo is the founder population of all germ layer derivatives and thus represents the bona fide in vivo pluripotent cell population. The so-called pluripotent state spans several days of development and is lost during gastrulation as epiblast cells make fate decisions towards a mesoderm, endoderm or ectoderm identity. It is now widely recognized that the features of the pluripotent population evolve as development proceeds from the pre- to post-implantation period, marked by distinct transcriptional and epigenetic signatures. During this period of time epiblast cells mature through a continuum of pluripotent states with unique properties. Aspects of this pluripotent continuum can be captured in vitro in the form of stable pluripotent stem cell types. In this review we discuss the continuum of pluripotency existing within the mammalian embryo, using the mouse as a model, and the cognate stem cell types that can be derived and propagated in vitro. Furthermore, we speculate on embryonic stage-specific characteristics that could be utilized to identify novel, developmentally relevant, pluripotent states. |
format | Online Article Text |
id | pubmed-5470286 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-54702862017-06-19 The many faces of Pluripotency: in vitro adaptations of a continuum of in vivo states Morgani, Sophie Nichols, Jennifer Hadjantonakis, Anna-Katerina BMC Dev Biol Review Pluripotency defines the propensity of a cell to differentiate into, and generate, all somatic, as well as germ cells. The epiblast of the early mammalian embryo is the founder population of all germ layer derivatives and thus represents the bona fide in vivo pluripotent cell population. The so-called pluripotent state spans several days of development and is lost during gastrulation as epiblast cells make fate decisions towards a mesoderm, endoderm or ectoderm identity. It is now widely recognized that the features of the pluripotent population evolve as development proceeds from the pre- to post-implantation period, marked by distinct transcriptional and epigenetic signatures. During this period of time epiblast cells mature through a continuum of pluripotent states with unique properties. Aspects of this pluripotent continuum can be captured in vitro in the form of stable pluripotent stem cell types. In this review we discuss the continuum of pluripotency existing within the mammalian embryo, using the mouse as a model, and the cognate stem cell types that can be derived and propagated in vitro. Furthermore, we speculate on embryonic stage-specific characteristics that could be utilized to identify novel, developmentally relevant, pluripotent states. BioMed Central 2017-06-13 /pmc/articles/PMC5470286/ /pubmed/28610558 http://dx.doi.org/10.1186/s12861-017-0150-4 Text en © The Author(s). 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Review Morgani, Sophie Nichols, Jennifer Hadjantonakis, Anna-Katerina The many faces of Pluripotency: in vitro adaptations of a continuum of in vivo states |
title | The many faces of Pluripotency: in vitro adaptations of a continuum of in vivo states |
title_full | The many faces of Pluripotency: in vitro adaptations of a continuum of in vivo states |
title_fullStr | The many faces of Pluripotency: in vitro adaptations of a continuum of in vivo states |
title_full_unstemmed | The many faces of Pluripotency: in vitro adaptations of a continuum of in vivo states |
title_short | The many faces of Pluripotency: in vitro adaptations of a continuum of in vivo states |
title_sort | many faces of pluripotency: in vitro adaptations of a continuum of in vivo states |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5470286/ https://www.ncbi.nlm.nih.gov/pubmed/28610558 http://dx.doi.org/10.1186/s12861-017-0150-4 |
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