Diverse Regulation of Vitamin D Receptor Gene Expression by 1,25-Dihydroxyvitamin D and ATRA in Murine and Human Blood Cells at Early Stages of Their Differentiation
Vitamin D receptor (VDR) is present in multiple blood cells, and the hormonal form of vitamin D, 1,25-dihydroxyvitamin D (1,25D) is essential for the proper functioning of the immune system. The role of retinoic acid receptor α (RARα) in hematopoiesis is very important, as the fusion of RARα gene wi...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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MDPI
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5486144/ https://www.ncbi.nlm.nih.gov/pubmed/28635660 http://dx.doi.org/10.3390/ijms18061323 |
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author | Janik, Sylwia Nowak, Urszula Łaszkiewicz, Agnieszka Satyr, Anastasiia Majkowski, Michał Marchwicka, Aleksandra Śnieżewski, Łukasz Berkowska, Klaudia Gabryś, Marian Cebrat, Małgorzata Marcinkowska, Ewa |
author_facet | Janik, Sylwia Nowak, Urszula Łaszkiewicz, Agnieszka Satyr, Anastasiia Majkowski, Michał Marchwicka, Aleksandra Śnieżewski, Łukasz Berkowska, Klaudia Gabryś, Marian Cebrat, Małgorzata Marcinkowska, Ewa |
author_sort | Janik, Sylwia |
collection | PubMed |
description | Vitamin D receptor (VDR) is present in multiple blood cells, and the hormonal form of vitamin D, 1,25-dihydroxyvitamin D (1,25D) is essential for the proper functioning of the immune system. The role of retinoic acid receptor α (RARα) in hematopoiesis is very important, as the fusion of RARα gene with PML gene initiates acute promyelocytic leukemia where differentiation of the myeloid lineage is blocked, followed by an uncontrolled proliferation of leukemic blasts. RARα takes part in regulation of VDR transcription, and unliganded RARα acts as a transcriptional repressor to VDR gene in acute myeloid leukemia (AML) cells. This is why we decided to examine the effects of the combination of 1,25D and all-trans-retinoic acid (ATRA) on VDR gene expression in normal human and murine blood cells at various steps of their development. We tested the expression of VDR and regulation of this gene in response to 1,25D or ATRA, as well as transcriptional activities of nuclear receptors VDR and RARs in human and murine blood cells. We discovered that regulation of VDR expression in humans is different from in mice. In human blood cells at early stages of their differentiation ATRA, but not 1,25D, upregulates the expression of VDR. In contrast, in murine blood cells 1,25D, but not ATRA, upregulates the expression of VDR. VDR and RAR receptors are present and transcriptionally active in blood cells of both species, especially at early steps of blood development. |
format | Online Article Text |
id | pubmed-5486144 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-54861442017-06-29 Diverse Regulation of Vitamin D Receptor Gene Expression by 1,25-Dihydroxyvitamin D and ATRA in Murine and Human Blood Cells at Early Stages of Their Differentiation Janik, Sylwia Nowak, Urszula Łaszkiewicz, Agnieszka Satyr, Anastasiia Majkowski, Michał Marchwicka, Aleksandra Śnieżewski, Łukasz Berkowska, Klaudia Gabryś, Marian Cebrat, Małgorzata Marcinkowska, Ewa Int J Mol Sci Article Vitamin D receptor (VDR) is present in multiple blood cells, and the hormonal form of vitamin D, 1,25-dihydroxyvitamin D (1,25D) is essential for the proper functioning of the immune system. The role of retinoic acid receptor α (RARα) in hematopoiesis is very important, as the fusion of RARα gene with PML gene initiates acute promyelocytic leukemia where differentiation of the myeloid lineage is blocked, followed by an uncontrolled proliferation of leukemic blasts. RARα takes part in regulation of VDR transcription, and unliganded RARα acts as a transcriptional repressor to VDR gene in acute myeloid leukemia (AML) cells. This is why we decided to examine the effects of the combination of 1,25D and all-trans-retinoic acid (ATRA) on VDR gene expression in normal human and murine blood cells at various steps of their development. We tested the expression of VDR and regulation of this gene in response to 1,25D or ATRA, as well as transcriptional activities of nuclear receptors VDR and RARs in human and murine blood cells. We discovered that regulation of VDR expression in humans is different from in mice. In human blood cells at early stages of their differentiation ATRA, but not 1,25D, upregulates the expression of VDR. In contrast, in murine blood cells 1,25D, but not ATRA, upregulates the expression of VDR. VDR and RAR receptors are present and transcriptionally active in blood cells of both species, especially at early steps of blood development. MDPI 2017-06-21 /pmc/articles/PMC5486144/ /pubmed/28635660 http://dx.doi.org/10.3390/ijms18061323 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Janik, Sylwia Nowak, Urszula Łaszkiewicz, Agnieszka Satyr, Anastasiia Majkowski, Michał Marchwicka, Aleksandra Śnieżewski, Łukasz Berkowska, Klaudia Gabryś, Marian Cebrat, Małgorzata Marcinkowska, Ewa Diverse Regulation of Vitamin D Receptor Gene Expression by 1,25-Dihydroxyvitamin D and ATRA in Murine and Human Blood Cells at Early Stages of Their Differentiation |
title | Diverse Regulation of Vitamin D Receptor Gene Expression by 1,25-Dihydroxyvitamin D and ATRA in Murine and Human Blood Cells at Early Stages of Their Differentiation |
title_full | Diverse Regulation of Vitamin D Receptor Gene Expression by 1,25-Dihydroxyvitamin D and ATRA in Murine and Human Blood Cells at Early Stages of Their Differentiation |
title_fullStr | Diverse Regulation of Vitamin D Receptor Gene Expression by 1,25-Dihydroxyvitamin D and ATRA in Murine and Human Blood Cells at Early Stages of Their Differentiation |
title_full_unstemmed | Diverse Regulation of Vitamin D Receptor Gene Expression by 1,25-Dihydroxyvitamin D and ATRA in Murine and Human Blood Cells at Early Stages of Their Differentiation |
title_short | Diverse Regulation of Vitamin D Receptor Gene Expression by 1,25-Dihydroxyvitamin D and ATRA in Murine and Human Blood Cells at Early Stages of Their Differentiation |
title_sort | diverse regulation of vitamin d receptor gene expression by 1,25-dihydroxyvitamin d and atra in murine and human blood cells at early stages of their differentiation |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5486144/ https://www.ncbi.nlm.nih.gov/pubmed/28635660 http://dx.doi.org/10.3390/ijms18061323 |
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