Downregulation of miR-503 Promotes ESCC Cell Proliferation, Migration, and Invasion by Targeting Cyclin D1
Esophageal squamous cell carcinoma (ESCC) is one of the most aggressive cancers in China, but the underlying molecular mechanism of ESCC is still unclear. Involvement of microRNAs has been demonstrated in cancer initiation and progression. Despite the reported function of miR-503 in several human ca...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5487524/ https://www.ncbi.nlm.nih.gov/pubmed/28602785 http://dx.doi.org/10.1016/j.gpb.2017.04.003 |
_version_ | 1783246481889689600 |
---|---|
author | Jiang, Lanfang Zhao, Zitong Zheng, Leilei Xue, Liyan Zhan, Qimin Song, Yongmei |
author_facet | Jiang, Lanfang Zhao, Zitong Zheng, Leilei Xue, Liyan Zhan, Qimin Song, Yongmei |
author_sort | Jiang, Lanfang |
collection | PubMed |
description | Esophageal squamous cell carcinoma (ESCC) is one of the most aggressive cancers in China, but the underlying molecular mechanism of ESCC is still unclear. Involvement of microRNAs has been demonstrated in cancer initiation and progression. Despite the reported function of miR-503 in several human cancers, its detailed anti-oncogenic role and clinical significance in ESCC remain undefined. In this study, we examined miR-503 expression by qPCR and found the downregulation of miR-503 expression in ESCC tissue relative to adjacent normal tissues. Further investigation in the effect of miR-503 on ESCC cell proliferation, migration, and invasion showed that enhanced expression of miR-503 inhibited ESCC aggressive phenotype and overexpression of CCND1 reversed the effect of miR-503-mediated ESCC cell aggressive phenotype. Our study further identified CCND1 as the target gene of miR-503. Thus, miR-503 functions as a tumor suppressor and has an important role in ESCC by targeting CCND1. |
format | Online Article Text |
id | pubmed-5487524 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-54875242017-07-12 Downregulation of miR-503 Promotes ESCC Cell Proliferation, Migration, and Invasion by Targeting Cyclin D1 Jiang, Lanfang Zhao, Zitong Zheng, Leilei Xue, Liyan Zhan, Qimin Song, Yongmei Genomics Proteomics Bioinformatics Original Research Esophageal squamous cell carcinoma (ESCC) is one of the most aggressive cancers in China, but the underlying molecular mechanism of ESCC is still unclear. Involvement of microRNAs has been demonstrated in cancer initiation and progression. Despite the reported function of miR-503 in several human cancers, its detailed anti-oncogenic role and clinical significance in ESCC remain undefined. In this study, we examined miR-503 expression by qPCR and found the downregulation of miR-503 expression in ESCC tissue relative to adjacent normal tissues. Further investigation in the effect of miR-503 on ESCC cell proliferation, migration, and invasion showed that enhanced expression of miR-503 inhibited ESCC aggressive phenotype and overexpression of CCND1 reversed the effect of miR-503-mediated ESCC cell aggressive phenotype. Our study further identified CCND1 as the target gene of miR-503. Thus, miR-503 functions as a tumor suppressor and has an important role in ESCC by targeting CCND1. Elsevier 2017-06 2017-06-09 /pmc/articles/PMC5487524/ /pubmed/28602785 http://dx.doi.org/10.1016/j.gpb.2017.04.003 Text en © 2017 Beijing Institute of Genomics, Chinese Academy of Sciences and Genetics Society of China http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Original Research Jiang, Lanfang Zhao, Zitong Zheng, Leilei Xue, Liyan Zhan, Qimin Song, Yongmei Downregulation of miR-503 Promotes ESCC Cell Proliferation, Migration, and Invasion by Targeting Cyclin D1 |
title | Downregulation of miR-503 Promotes ESCC Cell Proliferation, Migration, and Invasion by Targeting Cyclin D1 |
title_full | Downregulation of miR-503 Promotes ESCC Cell Proliferation, Migration, and Invasion by Targeting Cyclin D1 |
title_fullStr | Downregulation of miR-503 Promotes ESCC Cell Proliferation, Migration, and Invasion by Targeting Cyclin D1 |
title_full_unstemmed | Downregulation of miR-503 Promotes ESCC Cell Proliferation, Migration, and Invasion by Targeting Cyclin D1 |
title_short | Downregulation of miR-503 Promotes ESCC Cell Proliferation, Migration, and Invasion by Targeting Cyclin D1 |
title_sort | downregulation of mir-503 promotes escc cell proliferation, migration, and invasion by targeting cyclin d1 |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5487524/ https://www.ncbi.nlm.nih.gov/pubmed/28602785 http://dx.doi.org/10.1016/j.gpb.2017.04.003 |
work_keys_str_mv | AT jianglanfang downregulationofmir503promotesescccellproliferationmigrationandinvasionbytargetingcyclind1 AT zhaozitong downregulationofmir503promotesescccellproliferationmigrationandinvasionbytargetingcyclind1 AT zhengleilei downregulationofmir503promotesescccellproliferationmigrationandinvasionbytargetingcyclind1 AT xueliyan downregulationofmir503promotesescccellproliferationmigrationandinvasionbytargetingcyclind1 AT zhanqimin downregulationofmir503promotesescccellproliferationmigrationandinvasionbytargetingcyclind1 AT songyongmei downregulationofmir503promotesescccellproliferationmigrationandinvasionbytargetingcyclind1 |