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Frequency of Human papillomavirus in women attending cervical cancer screening program in Chile

BACKGROUND: Human papillomavirus (HPV) is the etiological factor for cervical cancer and its precursor lesions. The characterization of HPV genotypes in preneoplastic lesions and cervical cancer could establishes the effectiveness of vaccination plan in Chilean population. The aim of this study was...

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Autores principales: Brebi, Priscilla, Ili, Carmen Gloria, Andana, Alejandra, Menzel, Doris, Lopez, Jaime, Guzman, Pablo, Melo, Angelica, Buchegger, Kurt, Roa, Juan C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5543533/
https://www.ncbi.nlm.nih.gov/pubmed/28774281
http://dx.doi.org/10.1186/s12885-017-3496-x
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author Brebi, Priscilla
Ili, Carmen Gloria
Andana, Alejandra
Menzel, Doris
Lopez, Jaime
Guzman, Pablo
Melo, Angelica
Buchegger, Kurt
Roa, Juan C.
author_facet Brebi, Priscilla
Ili, Carmen Gloria
Andana, Alejandra
Menzel, Doris
Lopez, Jaime
Guzman, Pablo
Melo, Angelica
Buchegger, Kurt
Roa, Juan C.
author_sort Brebi, Priscilla
collection PubMed
description BACKGROUND: Human papillomavirus (HPV) is the etiological factor for cervical cancer and its precursor lesions. The characterization of HPV genotypes in preneoplastic lesions and cervical cancer could establishes the effectiveness of vaccination plan in Chilean population. The aim of this study was to determine HPV frequency in a group of women including in a cervical screening program in the public health care system in Chile. METHODS: We analyzed 985 cervical smears samples from women with different histological diagnosis, attending to public health care in Temuco-Chile between 2004 and 2012, to detect HPV genotypes, through PCR followed by reverse line blotting assay. RESULTS: HPV was found present in 80.8% (n = 796) of samples. Only a 5.6% of 985 samples were infected with a low-risk HPV, considering multiple infections. 10.5% (n = 8/76) of normal cervical epithelia, 83.5% (n = 208/249) and 87.6% (n = 557/636) of low and high grade squamous intraepithelial lesions, respectively, and 95.8% (n = 23/24) of squamous cervical carcinomas tested positive for HPV. HPV 16 was the most frequent genotype found (Overall 44.9%, n = 442/985; SCC: 62.5%, n = 15/24). A high variability of HPV types was also found in preneoplastic lesions, whereas there was a selection of genotypes in neoplasia. Also, there was a higher risk of infection with HPV 16 in women ≤26 years and 34–41 years old (p < 0.05), meanwhile infections with HPV 16 or HPV 18 have related with cancer development (p < 0.01). CONCLUSIONS: These data provide further information about the frequency of HPV genotypes in women with cervical lesions in Chile, and the introduction of new targeted vaccines against a wider spectrum of HPV is suggested. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12885-017-3496-x) contains supplementary material, which is available to authorized users.
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spelling pubmed-55435332017-08-07 Frequency of Human papillomavirus in women attending cervical cancer screening program in Chile Brebi, Priscilla Ili, Carmen Gloria Andana, Alejandra Menzel, Doris Lopez, Jaime Guzman, Pablo Melo, Angelica Buchegger, Kurt Roa, Juan C. BMC Cancer Research Article BACKGROUND: Human papillomavirus (HPV) is the etiological factor for cervical cancer and its precursor lesions. The characterization of HPV genotypes in preneoplastic lesions and cervical cancer could establishes the effectiveness of vaccination plan in Chilean population. The aim of this study was to determine HPV frequency in a group of women including in a cervical screening program in the public health care system in Chile. METHODS: We analyzed 985 cervical smears samples from women with different histological diagnosis, attending to public health care in Temuco-Chile between 2004 and 2012, to detect HPV genotypes, through PCR followed by reverse line blotting assay. RESULTS: HPV was found present in 80.8% (n = 796) of samples. Only a 5.6% of 985 samples were infected with a low-risk HPV, considering multiple infections. 10.5% (n = 8/76) of normal cervical epithelia, 83.5% (n = 208/249) and 87.6% (n = 557/636) of low and high grade squamous intraepithelial lesions, respectively, and 95.8% (n = 23/24) of squamous cervical carcinomas tested positive for HPV. HPV 16 was the most frequent genotype found (Overall 44.9%, n = 442/985; SCC: 62.5%, n = 15/24). A high variability of HPV types was also found in preneoplastic lesions, whereas there was a selection of genotypes in neoplasia. Also, there was a higher risk of infection with HPV 16 in women ≤26 years and 34–41 years old (p < 0.05), meanwhile infections with HPV 16 or HPV 18 have related with cancer development (p < 0.01). CONCLUSIONS: These data provide further information about the frequency of HPV genotypes in women with cervical lesions in Chile, and the introduction of new targeted vaccines against a wider spectrum of HPV is suggested. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12885-017-3496-x) contains supplementary material, which is available to authorized users. BioMed Central 2017-08-03 /pmc/articles/PMC5543533/ /pubmed/28774281 http://dx.doi.org/10.1186/s12885-017-3496-x Text en © The Author(s). 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Brebi, Priscilla
Ili, Carmen Gloria
Andana, Alejandra
Menzel, Doris
Lopez, Jaime
Guzman, Pablo
Melo, Angelica
Buchegger, Kurt
Roa, Juan C.
Frequency of Human papillomavirus in women attending cervical cancer screening program in Chile
title Frequency of Human papillomavirus in women attending cervical cancer screening program in Chile
title_full Frequency of Human papillomavirus in women attending cervical cancer screening program in Chile
title_fullStr Frequency of Human papillomavirus in women attending cervical cancer screening program in Chile
title_full_unstemmed Frequency of Human papillomavirus in women attending cervical cancer screening program in Chile
title_short Frequency of Human papillomavirus in women attending cervical cancer screening program in Chile
title_sort frequency of human papillomavirus in women attending cervical cancer screening program in chile
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5543533/
https://www.ncbi.nlm.nih.gov/pubmed/28774281
http://dx.doi.org/10.1186/s12885-017-3496-x
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