The role of CRP and ATG9B expression in clear cell renal cell carcinoma

The purpose of the study is to investigate the correlation between the expression of C-reactive protein (CRP) and autophagy-related 9B (ATG9B) and pathological features of clear cell renal cell carcinoma (CCRCC) patients. We also intended to explore the effects of manipulated expression of CRP and A...

Descripción completa

Detalles Bibliográficos
Autores principales: Ma, Zheng, Qi, Zengguang, Shan, Zhengfei, Li, Jiangsong, Yang, Jing, Xu, Zhonghua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Portland Press Ltd. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5686392/
https://www.ncbi.nlm.nih.gov/pubmed/28923830
http://dx.doi.org/10.1042/BSR20171082
_version_ 1783278776894881792
author Ma, Zheng
Qi, Zengguang
Shan, Zhengfei
Li, Jiangsong
Yang, Jing
Xu, Zhonghua
author_facet Ma, Zheng
Qi, Zengguang
Shan, Zhengfei
Li, Jiangsong
Yang, Jing
Xu, Zhonghua
author_sort Ma, Zheng
collection PubMed
description The purpose of the study is to investigate the correlation between the expression of C-reactive protein (CRP) and autophagy-related 9B (ATG9B) and pathological features of clear cell renal cell carcinoma (CCRCC) patients. We also intended to explore the effects of manipulated expression of CRP and ATG9B on the apoptosis and cell cycle progression of CCRCC cell line. ATG9B expression in CCRCC tissues and adjacent renal tissues was analyzed by immunohistochemistry (IHC). Gene expression was determined at transcription and translational levels using real-time quantitative PCR (RT-qPCR) and Western blot. The association between CRP/ATG9B expression and clinical-pathological parameters including age, gender, pathological grades, TNM stage and distant metastasis of the patients was assessed by correlation analysis. siRNA and overexpression plasmids construction were used to manipulate the expression of CRP in human CCRCC cell line 786-O. Cell apoptosis and cell cycle progression were determined using flow cytometry (FCM) and Hoechst 33258 staining. CRP expression correlates with ATG9B expression. The expression of CRP and ATG9B are significantly correlated with TNM staging, distant metastasis, and survival time of CCRCC patients. A high-level of CRP indicates a poor overall survival (OS). In addition, CRP expression influences cell cycle and apoptosis of CCRCC cells. The study reveals that CRP might be a CCRCC development promoter. In addition, there is a close relationship between CRP and ATG9B in CCRCC carcinogenesis.
format Online
Article
Text
id pubmed-5686392
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Portland Press Ltd.
record_format MEDLINE/PubMed
spelling pubmed-56863922017-11-27 The role of CRP and ATG9B expression in clear cell renal cell carcinoma Ma, Zheng Qi, Zengguang Shan, Zhengfei Li, Jiangsong Yang, Jing Xu, Zhonghua Biosci Rep Research Articles The purpose of the study is to investigate the correlation between the expression of C-reactive protein (CRP) and autophagy-related 9B (ATG9B) and pathological features of clear cell renal cell carcinoma (CCRCC) patients. We also intended to explore the effects of manipulated expression of CRP and ATG9B on the apoptosis and cell cycle progression of CCRCC cell line. ATG9B expression in CCRCC tissues and adjacent renal tissues was analyzed by immunohistochemistry (IHC). Gene expression was determined at transcription and translational levels using real-time quantitative PCR (RT-qPCR) and Western blot. The association between CRP/ATG9B expression and clinical-pathological parameters including age, gender, pathological grades, TNM stage and distant metastasis of the patients was assessed by correlation analysis. siRNA and overexpression plasmids construction were used to manipulate the expression of CRP in human CCRCC cell line 786-O. Cell apoptosis and cell cycle progression were determined using flow cytometry (FCM) and Hoechst 33258 staining. CRP expression correlates with ATG9B expression. The expression of CRP and ATG9B are significantly correlated with TNM staging, distant metastasis, and survival time of CCRCC patients. A high-level of CRP indicates a poor overall survival (OS). In addition, CRP expression influences cell cycle and apoptosis of CCRCC cells. The study reveals that CRP might be a CCRCC development promoter. In addition, there is a close relationship between CRP and ATG9B in CCRCC carcinogenesis. Portland Press Ltd. 2017-11-15 /pmc/articles/PMC5686392/ /pubmed/28923830 http://dx.doi.org/10.1042/BSR20171082 Text en © 2017 The Author(s). http://creativecommons.org/licenses/by/4.0/This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY) (http://creativecommons.org/licenses/by/4.0/) .
spellingShingle Research Articles
Ma, Zheng
Qi, Zengguang
Shan, Zhengfei
Li, Jiangsong
Yang, Jing
Xu, Zhonghua
The role of CRP and ATG9B expression in clear cell renal cell carcinoma
title The role of CRP and ATG9B expression in clear cell renal cell carcinoma
title_full The role of CRP and ATG9B expression in clear cell renal cell carcinoma
title_fullStr The role of CRP and ATG9B expression in clear cell renal cell carcinoma
title_full_unstemmed The role of CRP and ATG9B expression in clear cell renal cell carcinoma
title_short The role of CRP and ATG9B expression in clear cell renal cell carcinoma
title_sort role of crp and atg9b expression in clear cell renal cell carcinoma
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5686392/
https://www.ncbi.nlm.nih.gov/pubmed/28923830
http://dx.doi.org/10.1042/BSR20171082
work_keys_str_mv AT mazheng theroleofcrpandatg9bexpressioninclearcellrenalcellcarcinoma
AT qizengguang theroleofcrpandatg9bexpressioninclearcellrenalcellcarcinoma
AT shanzhengfei theroleofcrpandatg9bexpressioninclearcellrenalcellcarcinoma
AT lijiangsong theroleofcrpandatg9bexpressioninclearcellrenalcellcarcinoma
AT yangjing theroleofcrpandatg9bexpressioninclearcellrenalcellcarcinoma
AT xuzhonghua theroleofcrpandatg9bexpressioninclearcellrenalcellcarcinoma
AT mazheng roleofcrpandatg9bexpressioninclearcellrenalcellcarcinoma
AT qizengguang roleofcrpandatg9bexpressioninclearcellrenalcellcarcinoma
AT shanzhengfei roleofcrpandatg9bexpressioninclearcellrenalcellcarcinoma
AT lijiangsong roleofcrpandatg9bexpressioninclearcellrenalcellcarcinoma
AT yangjing roleofcrpandatg9bexpressioninclearcellrenalcellcarcinoma
AT xuzhonghua roleofcrpandatg9bexpressioninclearcellrenalcellcarcinoma