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Overexpressing STAMP2 attenuates adipose tissue angiogenesis and insulin resistance in diabetic ApoE(−/−)/LDLR (−/−) mouse via a PPARγ/CD36 pathway

The aim of this study was to investigate whether overexpression of STAMP2 improves insulin resistance by regulating angiogenesis in adipose tissues. The characteristics of diabetic mice were measured by serial metabolite and pathology tests. Samples were obtained from epididymal, subcutaneous and br...

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Autores principales: Wang, Feng, Han, Lu, Qin, Ran‐ran, Zhang, Yao‐yuan, Wang, Di, Wang, Zhi‐Hao, Tang, Meng‐Xiong, Zhang, Yun, Zhong, Ming, Zhang, Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5706521/
https://www.ncbi.nlm.nih.gov/pubmed/28631352
http://dx.doi.org/10.1111/jcmm.13233
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author Wang, Feng
Han, Lu
Qin, Ran‐ran
Zhang, Yao‐yuan
Wang, Di
Wang, Zhi‐Hao
Tang, Meng‐Xiong
Zhang, Yun
Zhong, Ming
Zhang, Wei
author_facet Wang, Feng
Han, Lu
Qin, Ran‐ran
Zhang, Yao‐yuan
Wang, Di
Wang, Zhi‐Hao
Tang, Meng‐Xiong
Zhang, Yun
Zhong, Ming
Zhang, Wei
author_sort Wang, Feng
collection PubMed
description The aim of this study was to investigate whether overexpression of STAMP2 improves insulin resistance by regulating angiogenesis in adipose tissues. The characteristics of diabetic mice were measured by serial metabolite and pathology tests. Samples were obtained from epididymal, subcutaneous and brown adipose tissues. Histological and morphological analysis demonstrated that STAMP2 gene overexpression reduced adipocyte size, angiogenesis in epididymal and brown adipose tissues. On aortic ring assay, microvessels sprouting from aortas were significantly inhibited after STAMP2 gene overexpression. The cellular effect of STAMP2 on angiogenesis was explored in human umbilical vein endothelial cells (HUVECs) model. Correlation of STAMP2 and angiogenesis was validated by Ad‐STAMP2 transfection and STAMP2 siRNA inhibition. In vitro, overexpression of STAMP2 significantly inhibited endothelial cell migration, tube formation. The effects of Ad‐STAMP2 transfection on HUVECs were abolished by treatment with PPARγ antagonist GW9662 (2.5 μM), and the roles of STAMP2 siRNA on HUVECs were also reversed by treatment with PPARγ agonist rosiglitazone (RSG) (0.1 mM). RT‐PCR indicated that STAMP2 could regulate levels of adhesion molecules, vascular endothelial growth factor A and CD36. The expression of PPARγ and CD36 was decreased when STAMP2 was inhibited by siRNA, while PPARγ and CD36 were highly expressed after overexpression of STAMP2. Our results suggested that STAMP2 gene overexpression may improve insulin resistance via attenuating angiogenesis in epididymal and brown adipose tissues through the PPARγ/CD36 signalling pathway.
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spelling pubmed-57065212017-12-06 Overexpressing STAMP2 attenuates adipose tissue angiogenesis and insulin resistance in diabetic ApoE(−/−)/LDLR (−/−) mouse via a PPARγ/CD36 pathway Wang, Feng Han, Lu Qin, Ran‐ran Zhang, Yao‐yuan Wang, Di Wang, Zhi‐Hao Tang, Meng‐Xiong Zhang, Yun Zhong, Ming Zhang, Wei J Cell Mol Med Original Articles The aim of this study was to investigate whether overexpression of STAMP2 improves insulin resistance by regulating angiogenesis in adipose tissues. The characteristics of diabetic mice were measured by serial metabolite and pathology tests. Samples were obtained from epididymal, subcutaneous and brown adipose tissues. Histological and morphological analysis demonstrated that STAMP2 gene overexpression reduced adipocyte size, angiogenesis in epididymal and brown adipose tissues. On aortic ring assay, microvessels sprouting from aortas were significantly inhibited after STAMP2 gene overexpression. The cellular effect of STAMP2 on angiogenesis was explored in human umbilical vein endothelial cells (HUVECs) model. Correlation of STAMP2 and angiogenesis was validated by Ad‐STAMP2 transfection and STAMP2 siRNA inhibition. In vitro, overexpression of STAMP2 significantly inhibited endothelial cell migration, tube formation. The effects of Ad‐STAMP2 transfection on HUVECs were abolished by treatment with PPARγ antagonist GW9662 (2.5 μM), and the roles of STAMP2 siRNA on HUVECs were also reversed by treatment with PPARγ agonist rosiglitazone (RSG) (0.1 mM). RT‐PCR indicated that STAMP2 could regulate levels of adhesion molecules, vascular endothelial growth factor A and CD36. The expression of PPARγ and CD36 was decreased when STAMP2 was inhibited by siRNA, while PPARγ and CD36 were highly expressed after overexpression of STAMP2. Our results suggested that STAMP2 gene overexpression may improve insulin resistance via attenuating angiogenesis in epididymal and brown adipose tissues through the PPARγ/CD36 signalling pathway. John Wiley and Sons Inc. 2017-06-19 2017-12 /pmc/articles/PMC5706521/ /pubmed/28631352 http://dx.doi.org/10.1111/jcmm.13233 Text en © 2017 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine. This is an open access article under the terms of the Creative Commons Attribution (http://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Wang, Feng
Han, Lu
Qin, Ran‐ran
Zhang, Yao‐yuan
Wang, Di
Wang, Zhi‐Hao
Tang, Meng‐Xiong
Zhang, Yun
Zhong, Ming
Zhang, Wei
Overexpressing STAMP2 attenuates adipose tissue angiogenesis and insulin resistance in diabetic ApoE(−/−)/LDLR (−/−) mouse via a PPARγ/CD36 pathway
title Overexpressing STAMP2 attenuates adipose tissue angiogenesis and insulin resistance in diabetic ApoE(−/−)/LDLR (−/−) mouse via a PPARγ/CD36 pathway
title_full Overexpressing STAMP2 attenuates adipose tissue angiogenesis and insulin resistance in diabetic ApoE(−/−)/LDLR (−/−) mouse via a PPARγ/CD36 pathway
title_fullStr Overexpressing STAMP2 attenuates adipose tissue angiogenesis and insulin resistance in diabetic ApoE(−/−)/LDLR (−/−) mouse via a PPARγ/CD36 pathway
title_full_unstemmed Overexpressing STAMP2 attenuates adipose tissue angiogenesis and insulin resistance in diabetic ApoE(−/−)/LDLR (−/−) mouse via a PPARγ/CD36 pathway
title_short Overexpressing STAMP2 attenuates adipose tissue angiogenesis and insulin resistance in diabetic ApoE(−/−)/LDLR (−/−) mouse via a PPARγ/CD36 pathway
title_sort overexpressing stamp2 attenuates adipose tissue angiogenesis and insulin resistance in diabetic apoe(−/−)/ldlr (−/−) mouse via a pparγ/cd36 pathway
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5706521/
https://www.ncbi.nlm.nih.gov/pubmed/28631352
http://dx.doi.org/10.1111/jcmm.13233
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