Target discovery screens using pooled shRNA libraries and next-generation sequencing: A model workflow and analytical algorithm
In the search for novel therapeutic targets, RNA interference screening has become a valuable tool. High-throughput technologies are now broadly accessible but their assay development from baseline remains resource-intensive and challenging. Focusing on this assay development process, we here descri...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5792015/ https://www.ncbi.nlm.nih.gov/pubmed/29385199 http://dx.doi.org/10.1371/journal.pone.0191570 |