Target discovery screens using pooled shRNA libraries and next-generation sequencing: A model workflow and analytical algorithm

In the search for novel therapeutic targets, RNA interference screening has become a valuable tool. High-throughput technologies are now broadly accessible but their assay development from baseline remains resource-intensive and challenging. Focusing on this assay development process, we here descri...

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Detalles Bibliográficos
Autores principales: Schaefer, Christiane, Mallela, Nikhil, Seggewiß, Jochen, Lechtape, Birgit, Omran, Heymut, Dirksen, Uta, Korsching, Eberhard, Potratz, Jenny
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5792015/
https://www.ncbi.nlm.nih.gov/pubmed/29385199
http://dx.doi.org/10.1371/journal.pone.0191570