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Chromogenic in situ Hybridization Compared with Real time Quantitative Polymerase Chain Reaction to Evaluate HER2/neu Status in Breast Cancer

BACKGROUND AND OBJECTIVE: The assessment of human epidermal growth factor receptor 2 (HER2) status has become of great importance in the diagnosis of breast cancer. The aim of this study was to investigate the diagnostic value of quantitative Polymerase Chain Reaction (qPCR) and Chromogenic In Situ...

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Autores principales: Ayatollahi, Hossein, Fani, Azar, Ghayoor Karimiani, Ehsan, Homaee, Fateme, Shajiei, Arezoo, Sheikh, Maryam, Shakeri, Sepideh, Shams, Seyyede Fatemeh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Iranian Society of Pathology 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5831068/
https://www.ncbi.nlm.nih.gov/pubmed/29515634
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author Ayatollahi, Hossein
Fani, Azar
Ghayoor Karimiani, Ehsan
Homaee, Fateme
Shajiei, Arezoo
Sheikh, Maryam
Shakeri, Sepideh
Shams, Seyyede Fatemeh
author_facet Ayatollahi, Hossein
Fani, Azar
Ghayoor Karimiani, Ehsan
Homaee, Fateme
Shajiei, Arezoo
Sheikh, Maryam
Shakeri, Sepideh
Shams, Seyyede Fatemeh
author_sort Ayatollahi, Hossein
collection PubMed
description BACKGROUND AND OBJECTIVE: The assessment of human epidermal growth factor receptor 2 (HER2) status has become of great importance in the diagnosis of breast cancer. The aim of this study was to investigate the diagnostic value of quantitative Polymerase Chain Reaction (qPCR) and Chromogenic In Situ Hybridization (CISH) to assess HER2 status of biopsy specimens. METHODS: To elucidate the status of HER2 gene amplification, biopsies of breast carcinoma from 120 patients with 2+ IHC status were analyzed by qPCR and CISH. RESULTS: The results of the two experiments were compared, and it was depicted that the concordance rate between CISH and qPCR assays was 88.1%.The quantification of HER2 gene with CISH and qPCR showed that there was a significant correlation (p value= 0.0001 and r= 0.808). CONCLUSION: The results of this research support the idea that qPCR is a precise and reproducible technique, which can be employed as a supplementary method to evaluate HER2 status.
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spelling pubmed-58310682018-03-07 Chromogenic in situ Hybridization Compared with Real time Quantitative Polymerase Chain Reaction to Evaluate HER2/neu Status in Breast Cancer Ayatollahi, Hossein Fani, Azar Ghayoor Karimiani, Ehsan Homaee, Fateme Shajiei, Arezoo Sheikh, Maryam Shakeri, Sepideh Shams, Seyyede Fatemeh Iran J Pathol Original Article BACKGROUND AND OBJECTIVE: The assessment of human epidermal growth factor receptor 2 (HER2) status has become of great importance in the diagnosis of breast cancer. The aim of this study was to investigate the diagnostic value of quantitative Polymerase Chain Reaction (qPCR) and Chromogenic In Situ Hybridization (CISH) to assess HER2 status of biopsy specimens. METHODS: To elucidate the status of HER2 gene amplification, biopsies of breast carcinoma from 120 patients with 2+ IHC status were analyzed by qPCR and CISH. RESULTS: The results of the two experiments were compared, and it was depicted that the concordance rate between CISH and qPCR assays was 88.1%.The quantification of HER2 gene with CISH and qPCR showed that there was a significant correlation (p value= 0.0001 and r= 0.808). CONCLUSION: The results of this research support the idea that qPCR is a precise and reproducible technique, which can be employed as a supplementary method to evaluate HER2 status. Iranian Society of Pathology 2017 2017-04-01 /pmc/articles/PMC5831068/ /pubmed/29515634 Text en © 2017, IRANIAN JOURNAL OF PATHOLOGY. This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Ayatollahi, Hossein
Fani, Azar
Ghayoor Karimiani, Ehsan
Homaee, Fateme
Shajiei, Arezoo
Sheikh, Maryam
Shakeri, Sepideh
Shams, Seyyede Fatemeh
Chromogenic in situ Hybridization Compared with Real time Quantitative Polymerase Chain Reaction to Evaluate HER2/neu Status in Breast Cancer
title Chromogenic in situ Hybridization Compared with Real time Quantitative Polymerase Chain Reaction to Evaluate HER2/neu Status in Breast Cancer
title_full Chromogenic in situ Hybridization Compared with Real time Quantitative Polymerase Chain Reaction to Evaluate HER2/neu Status in Breast Cancer
title_fullStr Chromogenic in situ Hybridization Compared with Real time Quantitative Polymerase Chain Reaction to Evaluate HER2/neu Status in Breast Cancer
title_full_unstemmed Chromogenic in situ Hybridization Compared with Real time Quantitative Polymerase Chain Reaction to Evaluate HER2/neu Status in Breast Cancer
title_short Chromogenic in situ Hybridization Compared with Real time Quantitative Polymerase Chain Reaction to Evaluate HER2/neu Status in Breast Cancer
title_sort chromogenic in situ hybridization compared with real time quantitative polymerase chain reaction to evaluate her2/neu status in breast cancer
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5831068/
https://www.ncbi.nlm.nih.gov/pubmed/29515634
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